The development of glufosinate-resistant soybean cultivars has created opportunities for use of glufosinate applied postemergence for weed control. Four field experiments were conducted in 2021 and 2022 to ascertain the effect of glufosinate rate and the addition of ammonium sulfate on annual weed control in glyphosate/glufosinate/2,4-D–resistant soybean. An increased glufosinate rate of 500 from 300 g ai ha−1 improved control of common ragweed, common lambsquarters, redroot pigweed, and foxtail species and resulted in decreased density and dry biomass of common lambsquarters and foxtail species. The addition of ammonium sulfate to glufosinate increased control of common lambsquarters, 2 and 8 wk after application (WAA), and of foxtail species, 2, 4, and 8 WAA, but did not improve control of common ragweed and redroot pigweed. Increasing the dose of glufosinate from 300 to 500 g ai ha−1 improves control of common ragweed, redroot pigweed, common lambsquarters, and foxtail species; however, the benefit of the addition of ammonium sulfate to glufosinate is weed species-specific.

Protoporphyrinogen oxidase (PPO)-inhibiting herbicides remain an important and useful chemistry 60 yr after their first introduction. In this review, based on topics introduced at the Weed Science Society of America 2021 symposium titled “A History, Overview, and Plan of Action on PPO Inhibiting Herbicides,” we discuss the current state of PPO-inhibiting herbicides. Renewed interest in the PPO-inhibiting herbicides in recent years, due to increased use and increased cases of resistance, has led to refinements in knowledge regarding the mechanism of action of PPO inhibitors. Herein we discuss the importance of the two isoforms of PPO in plants, compile a current knowledge of target-site resistance mechanisms, examine non–target site resistance cases, and review crop selectivity mechanisms. Consistent and reproducible greenhouse screening and target-site mutation assays are necessary to effectively study and compare PPO-inhibitor resistance cases. To this end, we cover best practices in screening to accurately identify resistance ratios and properly interpret common screens for point mutations. The future of effective and sustainable PPO-inhibitor use relies on development of new chemistries that maintain activity on resistant biotypes and the promotion of responsible stewardship of PPO inhibitors both new and old. We present the biorational design of the new PPO inhibitor trifludimoxazin to highlight the future of PPO-inhibitor development and discuss the elements of sustainable weed control programs using PPO inhibitors, as well as how responsible stewardship can be incentivized. The sustained use of PPO inhibitors in future agriculture relies on the effective and timely communication from mode of action and resistance research to agronomists, Extension workers, and farmers.

Herbicides are an important tool in managing weeds in turf and agricultural production. One of the earliest selective herbicides, 2,4-D, is a weak acid herbicide used to control broadleaf weeds. Water-quality parameters, such as pH and hardness, influence the efficacy of weak acid herbicides. Greenhouse experiments were conducted to evaluate how varying water hardness level, spray solution storage time, and adjuvant inclusion affected broadleaf weed control by 2,4-D dimethylamine. The first experiment evaluated a range of water-hardness levels (from 0 to 600 mg calcium carbonate [CaCO3] L−1) on efficacy of 2,4-D dimethylamine applied at 1.60 kg ae ha−1 for dandelion and horseweed control. A second experiment evaluated dandelion control from spray solutions prepared 0, 1, 4, 24, and 72 h before application. Dandelion and horseweed control by 2,4-D dimethylamine was reduced when the CaCO3 level in water was at least 422 or at least 390 mg L−1, respectively. Hard-water antagonism was overcome by the addition of 20 g L−1 ammonium sulfate (AMS) into the mixture. When AMS was included in spray mixtures, no differences were observed at 600 mg CaCO3 L−1, compared with distilled water. Spray solution storage time did not influence dandelion control, regardless of water-hardness level or adjuvant inclusion. To prevent antagonism, applicators should use a water-conditioning agent such as AMS when applying 2,4-D dimethylamine in hard water.

Off-target paraquat movement to rice has become a major problem in recent years for rice producers in the midsouthern United States. Nitrogen (N) fertilizer is applied to rice in greater quantity and frequency than all other nutrients to optimize rice yield. Two separate field studies were conducted from 2015 to 2018 in Stoneville, MS, to assess whether starter N fertilizer can aid rice recovery from exposure to a sub-lethal concentration of paraquat and to evaluate rice response to different N fertilizer management strategies following exposure to a sub-lethal concentration of paraquat. In both studies, paraquat treatments consisted of paraquat at 0 and 84 g ai ha–1 applied to rice in the two- to three-leaf (EPOST) growth stage. In the starter fertilizer study, N fertilizer at 24 kg ha–1 as ammonium sulfate (AMS) was applied to rice at spiking- to one-leaf (VEPOST), two- to three-leaf (EPOST), or three- to four-leaf (MPOST) growth stages before and after paraquat treatment. In the N fertilizer timing study, N fertilizer at 168 kg N ha–1 was applied in a single four-leaf to one-tiller (LPOST) application or two-, three-, and two four-way split applications. Despite starter N fertilizer applications, paraquat injured rice ≥41%, reduced height 57%, reduced dry weight prior to flooding 77%, delayed maturity 10 d, reduced dry weight at maturity 33%, and reduced rough rice yield 35% in the starter fertilizer study. Similarly, in the N fertilizer timing study, paraquat injured rice ≥45%, reduced height 14%, delayed maturity 10 d, reduced dry weight at maturity 44%, and reduced rough rice yield 50% for all N fertilizer management strategies. Both studies indicate that severe complications in growth and development can occur from rice exposure to a sub-lethal concentration of paraquat. In both studies, manipulation of N fertilizer management did not facilitate rice recovery from early-season exposure to paraquat.

Horseweed is a competitive summer or winter annual weed that produces up to 230,000 small seeds per plant that are capable of traveling more than 500 km via wind. Giant ragweed is a tall, highly competitive summer annual weed. Glyphosate-resistant (GR) horseweed and GR giant ragweed pose significant challenges for producers in the United States and Ontario, Canada. It is thought that an integrated weed management (IWM) system involving herbicide rotation is required to control GR biotypes. Halauxifen-methyl is a new selective broadleaf POST herbicide registered for use in cereal crops; there is limited information on its efficacy on horseweed and giant ragweed. The purpose of this research was to determine the efficacy of halauxifen-methyl applied POST, alone and in a tank mix, for the control of GR horseweed and GR giant ragweed in wheat across southwestern Ontario. For each weed species, an efficacy study consisting of six field experiments was conducted over a 2-yr period (2018, 2019). At 8 wk after application (WAA), halauxifen-methyl, fluroxypyr/halauxifen-methyl, fluroxypyr/halauxifen-methyl + MCPA EHE, fluroxypyr + MCPA ester, 2,4-D ester, clopyralid, and pyrasulfotole/bromoxynil + ammonium sulfate controlled GR horseweed >95%. Fluroxypyr and MCPA provided only 86% and 37% control of GR horseweed, respectively. At 8 WAA, fluroxypyr, fluroxypyr/halauxifen-methyl, fluroxypyr/halauxifen-methyl + MCPA EHE, fluroxypyr + MCPA ester, fluroxypyr/halauxifen-methyl + MCPA EHE + pyroxsulam, 2,4-D ester, clopyralid, and thifensulfuron/tribenuron + fluroxypyr + MCPA ester controlled GR giant ragweed 87%, 88%, 90%, 94%, 96%, 96%, 98%, and 93%, respectively. Halauxifen-methyl and pyroxsulam provided only 45% and 28% control of GR giant ragweed, respectively. Halauxifen-methyl applied alone POST in the spring controlled GR horseweed but not GR giant ragweed in winter wheat.

Methiozolin is a selective herbicide that has been reported to control annual bluegrass in creeping bentgrass putting greens. Golf course managers frequently tank-mix fertilizers with herbicides to reduce time and labor, but no information is available regarding such mixtures with methiozolin. Research was conducted to evaluate methiozolin for annual bluegrass control and creeping bentgrass safety when tank-mixed with ammonium sulfate or iron sulfate. Mixtures with ammonium sulfate did not influence annual bluegrass control while they did reduce creeping bentgrass injury in some instances. Mixtures with iron sulfate varied by experimental run but annual bluegrass control was either similar or increased while creeping bentgrass injury did not vary. Paclobutrazol was included as an alternative agrochemical comparison for annual bluegrass management; its application resulted in similar control and injury with and without iron sulfate addition, and injury and control were similar to methiozolin at appropriate rates. While some differences were observed, overall annual bluegrass and creeping bentgrass response to methiozolin was not affected by tank-mix nutrient partner relative to methiozolin applied alone.

Effects of 0.1 M ammonium sulfate, 0.1 M potassium phosphate, and 7 g/ha of the isopropylamine salt of imazapyr {(+)-2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-3-pyridinecarboxylic acid} were evaluated on the phytotoxicity of the ammonium salt of imazaquin {2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-3-quinolinecarboxylic acid} and the ammonium salt of imazethapyr {(+)-2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-ethyl-3-pyridinecarboxylic acid} applied postemergence to pitted morningglory (Ipomoea lacunosa L. #3 IPOLA). While each of these salts and imazapyr applied alone had no effect, combining them with imazaquin or imazethapyr significantly increased control of pitted morningglory.

Protoplasts were isolated from quackgrass to investigate the influence of two surfactants and ammonium sulfate on glyphosate uptake. Without adjuvants, the uptake of glyphosate was not detectable. The uptake of the reference compound 2-aminoisobutyric acid was linear with time and was inhibited 60% in the presence of 10 μM carbonyl cyanide m-chlorophenylhydrazone. Glyphosate uptake increased in the presence of both surfactants and ammonium sulfate. After 0.5 h of incubation the internal glyphosate concentration was 37% of the external concentration (100 μM) in the presence of 0.025 g L−1 Ethomeen T/25, 21% in the presence of 0.25 g L−1 Atplus 201, 13% with 5 mM ammonium sulfate, and 20% with 50 mM ammonium sulfate. The surfactant treatments did not reduce either the viability or the internal pH (= 7.1) as judged by fluorescein diacetate staining or 5,5-dimethyloxazolidine-2,4-dione distribution, respectively. The treatments with ammonium sulfate slightly increased the internal pH. The concentration of Ethomeen T/25 required to disrupt the protoplasts was 0.075 g L−1; Atplus 201 did not disrupt the protoplasts at concentrations up to 10 g L−1.

The effectiveness of preharvest applications of glyphosate on yellow toadflax was evaluated at five sites in Alberta from 1992 to 1994. At each site, glyphosate at 0.9 to 1.8 kg ae/ha was applied with or without nonionic surfactant and/or ammonium sulfate. Glyphosate at 2.7 kg/ha and glufosinate at 0.6 kg ai/ha were applied without additional adjuvants. The treatments were applied 7 to 10 d before crop harvest, when the majority of the yellow toadflax was in a flowering stage. Eleven to 12 mo after glyphosate application, yellow toadflax density was reduced by more than 80%. In most instances, there was no advantage in increasing the glyphosate rate above 0.9 kg/ha. The addition of nonionic surfactant and/or ammonium sulfate did not enhance glyphosate activity. Glufosinate did not control yellow toadflax in the year following treatment. Barley, canola, and flax yields in the year following applications were significantly higher in all preharvest glyphosate-treated plots than in untreated plots.

Studies were conducted to determine the usefulness of HOE-39866 (HOE-00661) in chemical fallow systems on the Canadian prairies. HOE-39866 at 0.5 to 1.0 kg ai/ha controlled Russian thistle, kochia, green foxtail, wild oats, and wheat comparable to paraquat, glyphosate, and glyphosate plus the isopropylamine salt of 2,4-D. However, control of barley with HOE-39866 was unacceptable. HOE-39866 was compatible in tank mixtures with ammonium sulfate, paraquat, chlorsulfuron, and metsulfuron. Ammonium sulfate improved weed control when HOE-39866 was applied at 0.25 kg/ha but not at 0.75 kg/ha. Adding paraquat at 0.07 to 0.21 kg ai/ha to HOE-39866 improved control of grass species over HOE-39866 alone. Adding chlorsulfuron and metsulfuron to HOE-39866 provided greater initial control of certain species as well as residual control of many weeds. HOE-39866 alone or in conjunction with other herbicides is an alternative to the herbicides used in chemical fallow systems.

The influence of adjuvants, inorganic salts, and herbicide formulations on the antagonistic effect of Na-bentazon on sethoxydim absorption was studied in quackgrass. Adjuvant BCH 815 00, at 2.4 L ha−1 alone or at 1.2 L ha−1 combined with 1.1 kg ha−1 of ammonium sulfate, added to the sethoxydim and Na-bentazon spray mixture overcame the antagonism on 14C-sethoxydim absorption in quackgrass. Crop oil concentrate at rates up to 9.6 L ha−1 did not overcome the antagonism. Adding ammonium phosphate or ammonium nitrate to the spray solution was as effective as adding ammonium sulfate in overcoming the antagonism caused by reduced absorption of sethoxydim even from the combination of Na-bentazon plus Na-acifluorfen. Ammonium salts and NH4-bentazon overcame the antagonism of Na-bentazon by forming readily absorbed NH4-sethoxydim.

The influence of ammonium sulfate on the activity of sethoxydim on wild oats and barley was examined under field conditions in 1989 and 1990. A chlorophyll assay, together with plant height and dry weight determinations, was used to quantify the visual ratings for sethoxydim activity. Ammonium sulfate enhanced the activity of 75 and 150 g ai ha-1 sethoxydim in 1989. In 1990, ammonium sulfate increased the activity of the lower rate of sethoxydim only. Of the three quantitative assays, chlorophyll concentration alone differentiated the more rapid development of injury symptoms with added ammonium sulfate that was observed in the visual ratings. The chlorophyll assay was not better than the visual rating assessment, however, and appears to offer no advantages that would justify the time required.

Laboratory experiments were conducted to assess the influence of surfactants applied with or without nitrogen on MON 37500 foliar absorption by Bromus tectorum, Bromus japonicus, Aegilops cylindrica, Triticum aestivum, Chorispora tenella, and Lactuca serriola. MON 37500 absorption in B. tectorum and B. japonicus increased from 40% 24 h after treatment (HAT) to 48% 48 HAT, averaged across surfactants with no added nitrogen. Averaged across nitrogen source and species, nonionic surfactant, ethylated seed oil, and organosilicate provided comparable enhancement of MON 37500 absorption (56 to 68%), whereas crop oil concentrate provided only 27 to 29% absorption under the same conditions. Averaged across species and surfactant class, urea ammonium nitrate had the greatest effect on MON 37500 absorption (68%), compared to ammonium sulfate (59%) or no nitrogen (40%). Nitrogen, regardless of the type, significantly improved foliar absorption of MON 37500. MON 37500 absorption by species was 71, 63, 57, 57, 49, and 38% in C. tenella, B. japonicus, T. aestivum, A. cylindrica, B. tectorum, and L. serriola, respectively, when averaged across surfactants and nitrogen. Densely pubescent B. japonicus leaves did not retain significant amounts of MON 37500 following a primary leaf wash.

Characteristics of glyphosate [N-(phosphonomethyl)glycine] uptake for as long as 3 days by plant cells were studied using suspension-cultured potato (Solanum tuberosum L. cv. ‘Red Pontiac’ and S. brevidens Phil.) cells. An experimental system was developed wherein uptake was assayed in a reduced salt medium that minimized cell growth yet maintained cell viability. Glyphosate uptake was nonlinear over time; an immediate rapid uptake phase was followed by uptake at a decreased rate that continued through 72 h. Glyphosate uptake was the same in both an experimental uptake medium (reduced salt and sucrose, no hormones) and in the complete growth medium used for routine cell growth. The pattern of glyphosate uptake was similar with both Red Pontiac and S. brevidens cells, though uptake was greater in Red Pontiac cells. Glyphosate uptake was not inhibited by 1 mM CaSO4, MgSO4, MnSO4, or ZnSO4. Rather, uptake was inhibited by the absence of salts at 8 h, and ZnSO4 and MnSO4 increased uptake. Similarly, increasing concentrations (0 to 5 mM) of CaSO4 and CaCl2 did not inhibit uptake, whereas the absence of either salt of Ca2+ decreased uptake. Increasing concentrations of (NH4)2 SO4 (0 to 150 mM) did not affect glyphosate uptake, but (NH4)2 SO4 at 50 mM and above was toxic to the cells.

Field experiments were conducted from 1993 to 1995 to compare weed control by the isopropylamine salt of glyphosate at 0.21, 0.42, 0.63, and 0.84 kg ae/ha applied at three stages of weed growth. Weed control by glyphosate applied at these rates alone or with ammonium sulfate at 2.8 kg/ha was also evaluated. In other experiments, potential interactions between glyphosate and acifluorfen, chlorimuron, and 2,4-DB were evaluated. Velvetleaf, prickly sida, sicklepod, pitted morningglory, entireleaf morningglory, palmleaf morningglory, and hemp sesbania were controlled more easily when weeds had one to three leaves compared with control when weeds had four or more leaves. Glyphosate controlled redroot pigweed, velvetleaf, prickly sida, sicklepod, and barnyardgrass more effectively than pitted morningglory, entireleaf morningglory, palmleaf morningglory, or hemp sesbania. Increasing the rate of glyphosate increased control, especially when glyphosate was applied to larger weeds. Greater variation in control was noted for pitted morningglory, palmleaf morningglory, prickly sida, and velvetleaf than for redroot pigweed, sicklepod, entireleaf morningglory, or hemp sesbania. Ammonium sulfate increased prickly sida and entireleaf morningglory control but did not influence sicklepod, hemp sesbania, or barnyardgrass control. Acifluorfen applied 3 d before glyphosate or in a mixture with glyphosate reduced barnyardgrass control compared with glyphosate applied alone. Chlorimuron did not reduce efficacy. Mixtures of glyphosate and 2,4-DB controlled sicklepod, entireleaf morningglory, and barnyardgrass similar to glyphosate alone.

Glyphosate, alone and in combination with other herbicides or additives, was evaluated as a spot or shielded treatment for the control of silverleaf nightshade in cotton in three experiments over four years. Glyphosate used at 10.8 g ae/L in 1985 on dryland cotton controlled 81 to 98% of the silverleaf nightshade, but cotton lint yields were reduced by all treatments compared to the weed-free check. Glyphosate used at 7.2 g ae/L in 1986 on irrigated cotton controlled 6 to 83% of the silverleaf nightshade, and lint yields were reduced only when multiple spot applications were made. Spot applications of glyphosate were effective for silverleaf nightshade control when applied 7 wk after crop emergence in 1988. Weed control and cotton injury were lower with shielded applications than spot applications. In 1989, retreatments during a season or the next year resulted in 95% or greater silverleaf nightshade control. Cotton yield increases from herbicide treatment were not observed in 1988; however, yield increases were observed in 1989 when spot treatments were applied to the more successful 1988 plots.

Certain salts in the spray carrier can antagonize sethoxydim, while other salts overcome the antagonism or increase the herbicide's efficacy. Sethoxydim responses to salts have not been consistent, and the inconsistency is not completely understood. Experiments were conducted in the greenhouse to determine sethoxydim phytotoxicity to oat as influenced by salts in the spray carrier and by air temperature at and immediately after application as they alter the spray deposit characteristics. Thick amorphous spray deposits were related to ineffective sethoxydim-petroleum oil adjuvant treatments applied alone at 10 C or with bentazon at 10 or 25 C. Also, thin deposits with salt residues were related to ineffective sethoxydim treatments, as when sodium bicarbonate was present. Ammonium sulfate reduced or overcame antagonism of sethoxydim phytotoxicity caused by low temperature, bentazon, and sodium bicarbonate and gave thin deposits having close contact with the leaf epicuticular surface but without obvious salt deposits. UV light antagonism of sethoxydim phytotoxicity was different depending on temperature at and shortly after application and depending on salts in the spray mixture. The results indicate that salts in a bentazon spray carrier are in part antagonistic because of their effect on final spray deposit.

Absorption, translocation, and metabolism of 14C-glufosinate were studied in three annual and two perennial weed species. Young seedlings of Setaria faberi, Chenopodium album, Cassia obtusifolia, Solanum carolinense, and Asclepias syriaca were treated with foliar-applied 14C-glufosinate, and plant tissues were harvested 12, 48, and 72 h after treatment (HAT). Absorption of 14C-glufosinate was initially rapid, but increased only slightly after 12 h in all species. Glufosinate absorption was highest in S. carolinense (73% of applied radioactivity), followed by S. faberi (54%), C. obtusifolia (44%), C. album (41%), and A. syriaca (37%) 72 HAT. Translocation of radioactivity out of the treated leaf was species dependent and did not increase much with time in all weed species. S. carolinense and S. faberi translocated the highest amounts of absorbed radioactivity out of the treated leaf with 49 to 59% moving to the upper foliage. S. faberi translocated the highest amount of absorbed radioactivity to the roots (12 to 14%), while C. album translocated the least (2 to 3%). TLC analysis of plant extracts showed that 14C-glufosinate was not metabolized in S. faberi, C. obtusifolia, S. carolinense, and A. syriaca. A glufosinate metabolite with an Rf value matching that of methyl-phosphinico propionate was detected in C. album. Treatment with ammonium sulfate (AMS) increased glufosinate absorption in S. faberi and C. obtusifolia 12 HAT, but decreased absorption in C. album. Treatment with pelargonic acid (PA) did not affect glufosinate absorption in any of the species tested. Treatment with AMS and PA did not affect glufosinate translocation in any of the five weed species. Treatment with AMS and PA did not influence the metabolism of glufosinate in any of the five weed species studied. These results show that differential absorption and translocation seem to explain the greater sensitivity of the annual and perennial weeds to glufosinate. Treatment with ammonium sulfate may increase the efficacy of glufosinate in perennial weeds.

Ammonium fertilizers, petroleum oil concentrate, and nonionic surfactant were evaluated as postemergence spray additives to improve giant foxtail and volunteer corn control by 28 g ai ha−1 of the ethyl ester of quizalofop or 56 g ha−1 sethoxydim. Additions of 0.25% by vol nonionic surfactant or 2.5% petroleum oil concentrate improved grass control, but additions of 10% urea ammonium nitrate (28-0-0), 10% ammonium polyphosphate (10-34-0), or 0.1M ammonium sulfate (21-0-0-24S) did not consistently affect grass control. In laboratory studies with corn, greatest 14C absorption from leaf-applied 14C-quizalofop (8 h after treatment) was found with additions of petroleum oil concentrate (80% absorbed) or nonionic surfactant (18% absorbed), while less absorption was observed with treatments containing either no additive, urea ammonium nitrate, ammonium polyphosphate, or ammonium sulfate (8 to 13% absorbed). Surface tension and droplet size of spray solutions were affected primarily by additions of nonionic surfactant, petroleum oil concentrate, and the formulated herbicides. Solution density, solute potential, pH, and buffering capacity were primarily affected by fertilizer additions.

Field and greenhouse experiments were conducted to determine effects of adding (NH4)2SO4 or urea ammonium nitrate containing 30% N (UAN) and/or substituting BCH 81508 S for crop oil with sethoxydim and sethoxydim plus bentazon applied to corn and large crabgrass. Control was similar with sethoxydim plus crop oil and sethoxydim plus BCH 81508 S. Adding (NH4)2SO4 or UAN moderately improved control with sethoxydim in some experiments. Both (NH4)2SO4 and UAN increased control when added to sethoxydim plus bentazon, with UAN being the more effective ammonium fertilizer. Large crabgrass control with sethoxydim plus bentazon was enhanced more with 1.4 and 2.8 kg/ha (NH4)2SO4 than with 5.6 kg/ha. Substituting BCH 81508 S for crop oil with sethoxydim plus bentazon also increased corn and large crabgrass control. Adding ammonium fertilizers plus substituting BCH 81508 S for crop oil with sethoxydim plus bentazon eliminated antagonism on corn and partially alleviated antagonism on large crabgrass.