The swimming path of a reared Pacific bluefin tuna, Thunnus orientalis, was measured in a submerged aquaculture net cage to understand how reared fish use the space in such a cage. A bluefin tuna (fork length, FL, 0.51 m) was captured by angling in the cage, and two micro data loggers (PD3GT, Little Leonardo; DST Comp-Tilt, Star-Oddi) were attached to its body. The fish was then released back into the net cage. The PD3GT measured its swimming speed and depth at 1-s intervals and recorded these in flash memory. The DST Comp-Tilt measured the magnetic field strength at 1-s intervals and recorded the heading estimated from the magnetic field strength in flash memory. The fish moved through the water in the cage at speeds of 0.7–0.8 m s-1 and attained a maximum speed of 3.6 m s-1. Burst swims exceeding 2 m s-1 were confirmed only after dark and a significant difference was found between the daytime and night-time swimming speeds (p < 0.001). The fish moved at depths between 2 and 22 m, swimming near the bottom during the day and at 10–15 m at night, with a significant difference in swimming depth between day and night (p < 0.001). The swimming path reconstructed by dead reckoning was visualised using night-time data. For this period, the absolute speed was corrected from 0.75  ±  0.09 m s-1 to 0.71  ±  0.15 m s-1 by removing the accumulated error from the reconstruction vector. This study allowed us to examine the behaviour of a tagged tuna in three dimensions and is the first to monitor the behaviour of a bluefin tuna in a submerged net cage. Although only one fish was analysed, this study provides useful information on the space use of reared fish in aquaculture net cages. Future studies must obtain sufficient data to understand the underlying generalities of tuna behaviour.

We used a digital stereo-video camera system for three-dimensional monitoring of cultured Pacific bluefin tuna, Thunnus orientalis, swimming freely in a net cage. We estimated the fork length and length frequency distribution of individual fish using the direct linear transformation (DLT) method. Information obtained from stereo images is useful for managing the growth of tuna during rearing. Our aim was to develop a simple method involving a combination of DLT and commercial image-processing software to enable aquaculturists to obtain three-dimensional measurements of fish. In this study, we used a stereo-video camera system to evaluate the precision and validity of fish size estimates determined from repeated measurements. Of the total assessed individuals swimming within a distance of  <5.5 m from the camera system, estimates for 99% (106/107) were found to be valid, with an error ratio (standard error/mean) of  <5%. Therefore, we believe that our proposed simple method for monitoring free-swimming fish could be very useful for aquaculture management.

To reveal the kinematical aspects of schooling development in the Pacific bluefin tuna Thunnus orientalis, changes in its schooling behaviour traits, local behaviour transmission among school members and morphological traits were investigated as fish developed from the larval to the juvenile stage. Schooling was first observed at around 24 days post-hatching (27 mm body length) in T. orientalis. Behaviour transmission among individuals took much longer among 24 d post-hatching individuals than among older fish. The compactness and polarity of the school progressively improved as the time required for behaviour transmission decreased. One cause of the reduction in the time required for behaviour transmission was the development of manoeuvrability, which resulted from further morphological development of the caudal fin and other organs related to swimming.

Reared sea bass were submitted to six stressful situations: hyperoxia with or without hypercapnia, increased stocking density in an open flow or recirculating system, transfer to another tank and nodavirus injection. The potentially negative impact of these factors on the lives of sea bass was investigated by measuring 9 water parameters and 19 fish parameters including total serum protein (TSP). TSP has already been used to evaluate stress. The present study investigates data of previous and new experiments, concentrating on the potential use of TSP as a routine indicator to assess welfare in sea bass reared on fish farms. In the current experiment, oxygen and carbon dioxide were seen to affect levels of TSP, but alterations were too erratic to enable proper comparison, probably because they are normal components of the fish environment and become toxic only by dose increase. TSP decreased when stocking density increased. After transfer to another tank, TSP decreased to 14% for three weeks and then increased during the fourth week through compensative overproduction, before returning to normal levels after 2 months. The results confirmed that transfer is an important stress factor for fish, with cumulative effects for successive transfers. TSP alteration of nodavirus-injected fish depends on the type of symptoms, which can be divided into 3 groups: (i) dying fish, in which TSP increased sharply due to over-production of protein involved in non-specific defences and inflammation, then decreased dramatically before death; (ii) whirling fish, a group that included both fish that later died, in which TSP decreased, and fish that subsequently survived, in which TSP increased due to development of specific immunity; and (iii) asymptomatic fish, in which TPS was similar to control levels and which were probably insensitive to nodavirus and/or had developed defences. In this paper, different mechanisms of TSP alteration are proposed and the interest of TSP as a field parameter is discussed. TSP is a non-destructive parameter that is robust, easy to measure everywhere and cheap, representing a suitable way of monitoring the overall welfare of fish by its regular increase. It can be used only as a “warning” of poor rearing conditions, however, and further investigations would be needed to identify the specific stress or health disorder.

The pressures on the use of the seashore are steadily rising, not only in developed countries but worldwide. Anthropogenic activity has long impacted the marine continental shelf down to a depth of approximately –200 m. New activities are now affecting this coastal space such as renewable energies, recreational uses and aquaculture in addition to the traditional ones of navigation or fishing. This evolution raises new sources of conflict amongst users which can require state involvement in order to manage the different stakeholders and pressure groups. However, the coastal space still offers a large potential for development for two reasons. Firstly, the physical three dimensional potential of this space enables the whole water column to be used, principally to increase the fishing productivity as in Japan. Secondly, innovative synergies can be created between socio-technical and ecological uses (a “fourth dimension”) such as the eco-design of wind turbine foundations in order to create fish habitat or sea grass settlement. This new vision in “4D” for the design and the management of coastal infrastructure can potentially reduce the risk of conflict as different uses of the coastal space would not necessarily exclude one another. Indeed, several forms of synergy could be developed such as fisheries with aquaculture or biological sustainability with social acceptability. Until now, limited attempts at such an approach have been done. We suggest this is likely due to the absence of a common eco-engineering vision and the lack of experience amongst biologists and engineers in the co-construction of projects. This eco-engineering, or “green” vision, also takes into account the complexity and resilience of the ecosystem in the long term, if underwater engineered infrastructures are also “eco”-designed to increase ecological gain This new conception, for development within the coastal area, provides for an increased bio-oriented complexity to engineered structure and therefore a better resistance of the ecosystem in the long term to anthropogenic pressures and a reduction in multi-user conflicts.

Tropical shrimp fisheries are characterized by various interactions with their natural environment and with other fisheries. These latter interactions can be explained by the high quantity of bycatch taken by industrial trawler fleets, which has a significant impact on fish populations associated with shrimps and thus also on finfish fisheries. Bycatch also includes emblematic species, which are subject to strict conservation measures decided by the international community. It seems important to identify and assess the biological and economic consequences of different mitigation measures (increase of mesh size, turtle excluder devices and bycatch reducing devices). This communication is based on case studies undertaken on the Indian white prawn (Fenneropenaeus indicus) and speckled shrimp (Metapenaeus monoceros) fisheries in Madagascar and on the brown shrimp (Farfantepenaeus subtilis) and pink spotted shrimp (Farfantepenaeus brasiliensis) fishery in French Guiana. A review of the impacts of these fisheries on resources and ecosystems is made and some results of experiments on mitigation devices given. Finally, the results of simulations obtained using a multi-species, multi-fleet, age-structured bioeconomic model, including modifications of catchability and costs related to the adoption of these devices, is presented and discussed.

We analyzed the small-scale fishery in the port of Vilanova i la Geltrú (NWMediterranean) to assess the fishing practices in this fishery and produce economicindicators of its status. We defined the fishing tactics (or métiers) of this fishery witha multivariate analysis approach for evaluating the main fishery resources and fishinggears employed. Our results show that netters in this fishery practice 7 types of fishingtactics. Five of these fishing tactics, in terms of target species, gear and seasonality,are also used in other small-scale Mediterranean fisheries. The main types of fishing gear(trammel nets, gillnets) and target species (Sepia officinalis,Solea vulgaris, Mullus surmuletus andMerluccius merluccius), and a well defined seasonality, are alsoobserved in other studies of Mediterranean small-scale fisheries. We show that theseasonal rotation of fishing tactics is determined by the availability of different fishspecies rather than market price. Identifying the fishing tactics, which in theMediterranean are relatively limited in number, of small-scale fisheries providesinformation for developing monitoring and management strategies for these fisheries. Wealso assessed the economic performance of the small-scale fishery in Vilanova i la Geltrúand its conflicts with other uses of the coastal zone. We conclude that small-scalefisheries are an economically viable activity although seriously threatened by concurrentuses of the coastal zone (conflict with other extractive activities; competition for spacewith tourism and leisure activities; pollution). The integrated management of the uses ofthe coastal zone is necessary in order to sustain small-scale fisheries, which have becomeless important in economic terms, but which are an important social component of localcoastal communities and are a source of high quality seafood with a low ecological impact.

The Chilean hake (Merluccius gayi gayi) is the predominant groundfishspecies inhabiting the southern Humboldt, mainly from Coquimbo (29° S) to Puerto Montt(42° S). At present there is only limited knowledge on the spatial distribution ofjuveniles of this species, particularly concerning its dependence on key physicalcharacteristics. On the basis of annual surveys carried out during austral winters from1997 to 2006, changes in the presence of juveniles were studied using Generalized AdditiveModel techniques. Temporal factors and spatial effects were more important than singlephysical variables in explaining the presence of juvenile hake (<34 cm totallength). Juvenile hake had a preference for shallower waters. Although salinity and oxygenwere significant, their contribution was marginal. Juvenile hake seem to prefer shallowoxygenated waters, but the nonlinear relationships and partial secondary peaks detected insalinity and oxygen probably only reflected the influence of three water masses found inthe winter habitat. Endogenous factors could be more important in determining temporal andspatial changes in the proportion of juvenile hake. An important change in the presence ofjuveniles has occurred since 2004, coinciding with a significant decline in biomass ofadult hake. It is postulated that cannibalism pressure from adults has probably declinedand that spatial and temporal changes in the presence of juvenile hake seem to be moreassociated with fishery-induced demographic effects.

Flatfishes represent an important resource in Portuguese fisheries. Although flatfish landings represent a low percentage of total fish weight landed, their importance is higher when commercial value is considered (11%). Official data on flatfish landings from 1992 to 2005 for all landing ports in Portugal were analysed, together with vessel characteristics, in order to detect patterns in flatfish fisheries. Fleet characteristics were heterogeneous, but most of the vessels were multi-gear. Three landing profiles were identified in the flatfish fishery, and the target species of these métiers showed variation in space and in time. Small vessels caught species that occur in coastal areas, mainly soles, bastard sole, flounder, turbot and brill, and usually also presented high landings of octopuses, cuttlefish and rays. Megrims and spotted flounder were caught with others species, like small pelagic fishes and seabreams by coastal trawlers. For this fleet component, catches of flatfish were usually bycatch. Generalized Linear Models (GLM) were used to analyse flatfish landings (LPUE, landings per unit effort and landings in terms of value) between 1992 and 2005 and to evaluate their relationships with several variables. The main effects in the models included year, month, landing port, vessel length class and total landings (kg). The models explained between 15% and 60% of the variability of the LPUE, and 46% to 82% of the variability of landing value, for the flatfish groups considered, with the most important factors being landing port, vessel length class, month, total landing (kg) and landing port: vessel length interaction. These results suggest high spatial and temporal variability. The results of this study may have implications for fishery management, because the LPUE was highest during the important periods of flatfish life cycles, like the spawning season. This fishing pattern has a negative impact on the stocks because of increased fishing pressure during a sensitive period for these species. The adoption of spatial and temporal closures should be implemented.

The Central Mediterranean provides important neritic habitats for loggerhead turtles (Caretta caretta), but Mediterranean bottom trawlers catch an estimated 30 000 turtles a year, with 25% mortality. Mortality by trawling is mainly due to enforced apnoea during towing activity. In order to reduce the submergence time and consequent turtle mortality, a specific technical modification was developed in the early 1980s: the Turtle Excluder Device (TED). In this paper, we field-tested a typical Supershooter TED and three new types of low-cost TED, built with different designs and materials, incorporating aspects of US and Australian TEDs, as well as design features to improve handling and catch rates. The performance of the TEDs was investigated under commercial fishing conditions in diverse trawling grounds in the Adriatic Sea (Mediterranean). All TEDs were easy to operate and did not require changes to normal fishing operations. Due to lack of entry of turtles it was not possible to evaluate the ability of the different TEDs to release turtles, but one large loggerhead turtle (C. caretta) was captured during the experimental tows and was successfully excluded by the Supershooter. The TEDs reduced anthropogenic debris and, consequently, sorting operations on board. Among the four TEDs tested, both the semi-rigid TED and the Supershooter performed in accordance with the design objectives: total discards were reduced but total commercial catches were not significantly reduced. With the Supershooter, all European hake (Merluccius merluccius) individuals equal to or above 16 cm were found in the codend and 10–15% of those between 5.0 and 15.5 cm were released. In general, the total discard rate of the TED-equipped nets was reduced to around 20–60%. Since the Council Regulation (EC) No. 1967/2006 called for a discard reduction policy in waters under the jurisdiction of the European Union, TEDs may have some broader value in this context.

Argentina is one of the countries with the largest overall landings of skates and rays(24 000 t/year). As a consequence of high levels of exploitation and deficient managementtools, many of these species are threatened with extinction. In the bottom trawl fisheryof San Matías Gulf, northern Patagonia, skates and rays are caught as bycatch and all havehistorically been recorded in the fishery logbook under the category “rays”. Therefore,species composition and their characteristics were unknown. The aim of this study was tocharacterize the commercial exploitation of batoid species in this fishery. Our resultsindicate that commercial landings include nine species of skate, six of which are common.Among these, Atlantoraja platana, Sympterygia bonapartiiand Dipturus chilensis were considered the main species of thisfishery. Their combined relative abundance and weight made up about 90% of the batoidcatch throughout the year. Sexual segregation was detected in four species in whichindividuals of one sex dominated commercial landings. The commercial fraction was composedof individuals with a wide range of sizes. Immature individuals could represent 90% of theindividuals landed for some species. At present, lack of data prevents us from quantifyingthe impact that this fishery is having on these species, but our findings highlight theimportance of conducting research surveys to assess the abundance and geographic extent ofthese populations. The information presented here provided the basis for the proposal of anew logbook format incorporating species-level identification. The new logbook wasimplemented on 1 January 2010.

Seaward migration of immature salmonids (smolts) may be associated with severe mortality in anthropogenically altered channels. Few studies however, have identified distinct behaviours that lead to exposure to adverse habitats or even unsuccessful migration. This study used high resolution telemetry to map migration routes of Atlantic salmon (Salmo salar) smolts approaching a water withdrawal zone associated with an aquaculture facility in a lowland river. Individual smolts were tagged with an acoustic transmitter and released upstream of the water withdrawal zone. A trap was installed downstream of the water withdrawal zone. The trap captured all smolts that passed the water withdrawal zone. The tracking results confirmed previous studies on Pacific salmon showing that Atlantic salmon smolts may perform milling behaviours (i.e. upstream excursions and circular swimming behaviour) in anthropogenically altered channels. Non-milling and milling smolts were compared. Smolts performing milling behaviours covered a larger area (m2) and experienced an increased probability of entering the water withdrawal zone, considered an adverse habitat. Finally, smolts were identified as either passing (67%) or non-passing (33%) the water withdrawal zone based on the recapture data from the trap. In total, 20% of the non-passing smolts entered the aquaculture facility. Several behavioural traits differed between the remaining (80%) non-passing smolts and the passing smolts. In particular, time spent near the water withdrawal zone correlated negatively with the probability of passage. These links between individual behaviours and exposure to adverse habitats and passage probability may be applied to improve management of salmonid populations.

Acute toxicity of ammonia was investigated in four life stages of juvenile chub, Leuciscus cephalus (cyprinid fish): 1, 10, 20 and 30 days after the first feeding. The fish used for the toxicity test were reared intensively in a closed recirculation system. Each acute toxicity test duration was 96 h and lethal concentration LC1, LC50 and LC99 values were calculated for 24, 48, 72 and 96 h. The susceptibility of chub to acute ammonia toxicity decreased linearly with age and stage of development. The LC50 (48 h) values ranged from 0.62 mg L-1 of unionized ammonia nitrogen for one day after first feeding larvae to 1.73 mg L-1 for 30 days after first feeding ones. A significant linear relationship between chub larvae susceptibility to ammonia toxicity and both body weight and length was found. The critical level of unionized ammonia nitrogen for chub larvae was suggested as 0.49 mg L-1.

Cryopreservation is a valuable tool for genetic improvement programs. Several bivalvemollusc species have already been the subject of such programs and the Tahitian blackpearl oyster industry is now planning the development of selective breeding for desirabletraits in Pinctada margaritifera. The ability to cryopreserve spermatozoawould, therefore, offer significant benefits to the cultured black pearl industry.Spermatozoa were cryopreserved with cryoprotectant agent (CPA) 0.7 M trehalose in 0.8 MMe2SO and a two-step freezing process was used: straws were first maintainedin nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and storedfor one week before use. The viability of thawed sperm was 23% lower than that of freshsperm. When using thawed sperm, therefore, a higher sperm/egg ratio of 100 000:1 wasrequired to reach 80% oocyte fertilization, compared with 100:1 for fresh sperm.Nevertheless, this first demonstration of cryopreserved sperm fertility in black pearloyster confirms the hatchery applicability of the cryopreservation technique defined here.Monitoring for larval viability during the first 23 days of life revealed no significantdifferences between the progeny produced with cryopreserved sperm and that produced usingfresh sperm.