Methods to assess changes in the mental state of animals in response to their environment can be used to provide information to enhance animal welfare. One of the most profound changes of mental state observable in mammals is the change between wakefulness and sleep. Sleeping mammals have characteristics that are similar to one another and are measurable, such as specific behaviours, changes in responsiveness to external stimuli and changes in electrophysiology and neurochemistry. Although sleep is a ubiquitous behaviour in the life of mammals, there has been relatively little research on this topic in domesticated animals. All animals are motivated to sleep and this motivation increases after a prolonged period of wakefulness. In humans, sleep can be affected by what has occurred in the prior period of wakefulness and this has also been demonstrated in some non-human mammals. An important aspect of human sleep medicine is the association between stress and subsequent sleep disturbances. Studying changes in amount, bout length, distribution or type of sleep after exposure to potentially stressful events, could help us understand how animals respond to changes in their environment. It is possible that different types of stressors could affect sleep characteristics in different ways and that monitoring and identifying these changes could be useful in providing an additional way of identifying management procedures that have the potential to affect welfare. Sleep measurement is a potentially valuable tool in studies to assess animal welfare.

This paper sought to determine whether common haematology and blood biochemistry values might assist in determining the relative welfare outcomes arising from the capture of red foxes (Vulpes vulpes) by treadle-snares, Victor Soft Catch® (VSC) #3 traps, cage traps, netting and sampling by shooting. Compared to all other capture methods and shooting, treadle-snared foxes had significantly higher mean albumin (ALB), creatine kinase (CK), red cell count (RCC), neutrophil to lymphocyte (N:L) ratio, sodium (Na), total protein (TP), white cell counts (WCC) and lower glucose (Gl). Treadle-snares were also associated with higher chloride (Cl), haemoglobin (Hb) and packed cell volume (PCV) than cage trapping and netting. Treadle-snares produced indicators of possible muscle damage, exertion and dehydration compared to cage and VSC traps. Cage trapping and netting produced lower indications of exertion, possible muscle damage and dehydration compared to both treadle-snares and VSC traps. These data do not support previous conclusions that due to similar injury scores, treadle-snares and VSC traps produced equivalent welfare outcomes. In restraining traps, injury and death sustained during capture are end-points of poor trapping welfare. Monitoring stress using physiological indicators allows the comparison of the relative potential for different capture techniques to cause pathological and pre-pathological states. As the response of physiological indicators to stress is not independent of time, accurate data on the duration of captivity and the relative intensity of struggling behaviour should be routinely collected when assessing the comparative humaneness of different trap devices.

Single housing is believed to be chronically stressful and to have a negative impact on welfare and cognition in rats (Rattus norvegicus). However, single housing does not consistently evoke stress-like responses nor does it consistently impair cognitive performance. In an experiment in which all cages were separated by an opaque barrier, single- and pair-housed pigmented (dark-eyed) rats performed equally in a cognitive test and displayed similar levels of anxiety during testing. Additionally, bar biting in the home cage did not differ between the two groups. Stress levels both during cognitive testing and in the home cage were higher than those we have previously reported when rats were housed without opaque barriers between the cages. We conclude that visual interactions between rats in different cages may be of sufficient significance that single housing in a cage with a view to neighbouring rats and to the rest of the laboratory holding room may be preferable to pair housing in a cage without this view.

Captive breeding of peccaries is on the increase in neotropical countries. Few studies, however, have reported behavioural responses of wild animals under farmed conditions. Therefore, the aim of this study was to evaluate the effects of space allowance on the occurrence of social behaviour patterns on farmed collared peccary (Pecari tajacu). We observed three herds of collared peccaries each containing eight acquainted individuals. Using a 3 * 3 Latin square design, herds were allocated, in a random order, to one of the three experimental enclosures, each with a different size: 375, 750 and 1,500 m2 of total available area, each with three wooden shelters. We recorded all the occurrences of selected positive and agonistic behavioural patterns that occurred 90 min before and during feeding. Enclosure size had a significant effect on agonistic patterns of peccaries during feeding, in that more agonistic behaviour was observed in smaller spaces. We also found that shelter usage increased as space decreased. Differing space allowances, however, did not have an effect on the occurrence of positive interactions that were more frequent before compared to during feeding. We concluded that enclosure size had an effect on the expression of agonistic behaviours and the use of shelters by collared peccaries. Thus, animal welfare can be improved by adopting at least 187.5 m2 per peccary. In addition, our study also confirmed the importance of shelter areas in collared peccary husbandry.

1080 (sodium fluoroacetate)-baiting programmes are an important and often the only option for reducing the impact of invasive vertebrate pests on biodiversity and agricultural production in Australia and New Zealand. These programmes are generally recognised as being target specific, and environmentally and user safe. Nevertheless, although 1080 has few recognised long-term side-effects, its potential to disrupt endocrine systems has been recently raised, and there is some conjecture regarding the humaneness of 1080 for certain target species. However, the assessment of the humaneness of any vertebrate pesticide must be commensurate with its mode of action, metabolism, target specificity, and operational use. This has not always occurred with 1080, particularly regarding these aspects, and its overall effects. The actual risk faced by non-target species during baiting operations is not accurately reflected simply by their sensitivity to 1080. 1080 is not endocrine-disrupting or carcinogenic, and because of the lag phase before signs of poisoning occur, the time from ingestion to death is not a reliable indicator of its humaneness. Moreover, functional receptors and neurological pathways are required to experience pain. However, as 1080 impairs neurological function, mainly through effects on acetylcholine and glutamate, and as this impairment includes some pain receptors, it is difficult to interpret the behaviour of affected animals, or to assess their ability to experience discomfort and pain. This has implications for assessing the merits of including ameliorative agents in 1080 baits aimed at further improving welfare outcomes. We also suggest that the assessment of the humaneness of any vertebrate pesticide should follow the ethical pest control approach, and on this basis, believe that the use of 1080 to reduce the detrimental impacts of invasive vertebrates is ethical, particularly with respect to the expectations of the wider community.

The poultry red mite (Dermanyssus gallinae) causes severe welfare concerns for laying hens arising from anaemia and disease transmission, and has been identified as an associated risk factor in cannibalistic feather pecking. Previous work suggests that essential oils may offer an alternative to synthetic acaricides to control D. gallinae. Such alternatives are needed due to the limitations of synthetic acaricides (eg availability, resistance, environmental concerns and product residues). The aim of the current study was to ensure that selected essential oils have no negative impact on either hen welfare or egg production. To achieve this aim, small groups of laying hens were confined in poultry huts for a period of eight weeks during which time the interior of the huts was sprayed at weekly intervals with one of four different treatments: i) Thyme essential oil at 5× the LC90 level (the concentration of oil previously found to kill 90% of D. gallinae under laboratory conditions) for D. gallinae in 500 ml of water, ii) Pennyroyal essential oil at 5× the LC90 level for D. gallinae in 500 ml of water, iii) Solvent-only (huts treated with 500 ml of water); and iv) Pseudo-spray where huts were not treated with any product, but subjected to sham-spraying. The results suggest that pennyroyal essential oil would not be suitable for further development as an acaricide for D. gallinae, since this treatment had to be terminated early in the study period as a result of concerns about the welfare of hens exposed to this oil. Conversely, there were few differences in feather condition, hen weight, feed intake, feeding efficiency, egg production or egg weight between thyme-treated huts and huts that were either pseudo-sprayed or sprayed with solvent-only (water). It is concluded that thyme essential oil is a promising candidate for further development as an acaricide for D. gallinae to help safeguard the welfare of laying hens in commercial poultry systems.

Marine ecosystems are exposed to a wide variety of factors that may produce disturbances in their structure and functioning. The Gulf of California supports fisheries, tourism, intensive agriculture, mining, and more recently, shrimp aquaculture. Under such conditions, animals are forced to cope with several changes in their environment that can contribute to animal welfare problems. Serum cortisol level analysis may be a particularly useful means of assessing the physiological status of mammals potentially affected by increasing human activity in the Gulf of California, such as the California sea lion (Zalophus californianus californianus). In this study, we report for the first time the serum cortisol concentration of free-living, wild California sea lion pups. The analysis was performed in eleven rookeries along the Gulf of California. Two consecutive blood samples (S1 and S2) were obtained from 56 sea lion pups following a capture, handling and anaesthesia regime, and cortisol was measured by radioimmunoassay. Female pups showed higher serum cortisol than males in the first sample. In males, the second sample was significantly higher than the first. Cortisol levels in the two samples of both sexes combined differed between the Southern and Midriff-region rookeries. This information could be useful to assess welfare in wild populations of sea lions and to determine anthropogenic factors in the Gulf of California that may contribute to stress and reduced welfare.

The objective of this study was to evaluate conditions during transport and their effects on welfare and carcase bruising of beef cattle in Uruguay, a country with an extensive, pasture-based, production system. Twelve abattoirs were visited periodically during two years (2002-2003) and 448 trucks were inspected on arrival. The state of vehicle maintenance was deemed ‘unacceptable’ for the transport of animals in 16.5 (± 3.2)% of cases and most of the vehicles (99.1 [± 0.1]%) had a ‘guillotine-type’ door at the rear end. Both characteristics showed a significant association with the presence of carcase bruising. No overall significant difference was found with the year of vehicle manufacture (24.6 [± 0.2]% before 1990), the presence of rollers bars in 51.3 (± 0.1)% of the trucks on one or both sides of the doors, and the working experience of the men who transported the cattle (> 10 years). The average distance travelled with the animals loaded onto trucks was 240 (± 9) km and the mean journey length was 305 (± 7) min (5 h). A statistically significant effect of the distance travelled and the state of the roads on the occurrence of bruising was found. The use of devices to force animals to move, such as: electric prod (75%), sticks (3%), loud shouts (40%) and a combination of all of the above were positively correlated with bruising. After slaughter, carcase bruising was identified, quantified, and classified into three degrees of muscle injury. From 15,168 beef cattle observed, 60.0% (9,106) had at least one injury. Of the injured carcases, 33.1% (3,015) had one bruise, 25.1% (2,289) had two bruises, 16.2% (1,474) had three bruises and 25.6% (2,328) four or more bruises. Transporting animals in a humane fashion will reduce the amount of bruising on carcases, thereby increasing both the welfare of beef cattle and the profitability of the beef industry in Uruguay.

The stunning and killing efficiency of ice, superchilling (N2 gas), freezing (-37°C), gradual heating (40°C), boiling, piercing of ganglia, salt baths (NaCl and KCl), gas (CO2) and electricity (50 Hz AC) on edible crabs was studied. Results showed that electricity was the most efficient stunning method, whereby edible crabs could be rendered insensible within 1 s using electric field strengths of 400 V m−1 and above. Prolonging the electrical current to 10 s resulted in less potential difference (220 V m−1) required to stun the crabs. Applying a two-stage stun with 530 Vm−1 for 1 s followed by 170 V m−1 for 2 min resulted in a state of prolonged unconsciousness and 60% mortality. Failure to stun the crabs with electricity resulted in massive autotomy, where all appendages were lost. Behavioural responses were lost in approximately 30% of crabs after 100 min of chilling on ice, while freezing did not render the crabs unconscious until temperatures of subzero were reached. The exposed chelipeds stiffened, and once frozen, irreversible damage was caused. Placing crabs into heated seawater (40°C) led all responses to be lost after 5 min, while the internal temperature exceeded an average of 26°C, representing approximately 2.5 min of boiling. Gas, in the form of CO2, NaCl, and a low concentration of KCl (5%), failed to render the animals insensible within 12 min. Using 20% KCl saw all animals lose all behavioural responses within 3 min. The piercing of single ganglions failed to kill the animal; both ganglia must be pierced in order to kill the animal. We conclude that electrical stunning is recommended prior to boiling or carving, while piercing can alternatively be carried out by trained personnel.

A total of 560 cattle and water buffalo were examined at livestock markets in Bangladesh. The aim was to assess the frequency of nose and tail injuries that could have occurred during handling and transport. The frequency of nose piercing was 64%, and 69% of the cattle and 54% of the water buffalo had rubbing or tearing injuries at the nostrils from nose ropes fitted through the pierced hole. Almost half of the nose-pierced animals (47%) had lacerations and ulcerations where the nose rope had rubbed against the nose. Pus at the nostril was observed in 56% of the animals, and 57 and 58% had severe and extended nose injuries, respectively. Nose injuries were more severe in imported Hariana cattle compared with exotic and local breeds. In cattle plus water buffalo, 39% had tail injuries of which the two major abnormalities were absent tail end (2%) and kinked tail (98%). Tail injuries were more common in cattle (51%) than water buffalo (15%). Among the cattle breeds, the frequency of tail injuries in Hariana was 65%. In conclusion, the cattle and water buffalo experienced a number of serious injuries and this calls for greater attention to be paid to handling methods in draught animals in south Asia.

The effects of presumed long distance transport on blood parameters were examined in cattle and water buffalo exported from India to Bangladesh. The aim was to assess the welfare of the animals in terms of the frequency of dehydration, metabolic depletion and muscle injury or activation, when they reached their destination. The physiological indicators were total plasma protein, serum sodium, plasma glucose, serum non-esterified fatty acids (NEFA) and serum creatine kinase (CK). Ninety-eight cattle and 57 water buffalo were randomly selected from 24 trucks as they arrived at the market between 0700 and 1000h each day during the hot season (April). In the majority of animals, all five blood variables were higher than published normal values. In all the cattle, total plasma protein concentration, serum NEFA concentration and serum CK activity were higher than the normal range and these values were higher than normal in over 84% of the buffalo. Seventy-two percent of all the animals were hypernatraemic. About 90% of the buffalo and 86% of the cattle were hyperglycaemic. There were no differences in total plasma protein and serum sodium between cattle and water buffalo. By contrast, cattle had significantly higher NEFA and CK and lower glucose than water buffalo. It was evident that this long distance export trade was associated with dehydration, lipolysis and muscle injury or activation. It is recommended that both cattle and water buffalo are given adequate feed and water whenever they are off-loaded from vehicles during the course of journeys.

Positive reinforcement training is one component of behavioural management employed to improve psychological well-being. There has been regulatory promotion to compensate for restricted social housing in part by providing human interaction to singly caged primates, implying an efficacy standard for evaluating human interaction. The effect of positive reinforcement training on the behaviour of 61 singly housed laboratory rhesus macaques (Macaca mulatta) was evaluated at two large primate facilities. Training involved body part presentation and basic control behaviours. Baseline data were compared to two treatment phases presented in varying order across individuals, six minutes per week of positive reinforcement training and six minutes per week of unstructured human interaction. While a MANOVA involving behavioural categories and study conditions across study subjects was significant, univariate ANOVAs found no effect of phase within any behavioural category. Categorising subjects according to rearing, housing facility, or baseline levels of abnormal behaviour did not reveal changes in behaviour with positive reinforcement training or human interaction. This study failed to detect, to any degree, the types of behavioural changes documented in the scientific literature to result from pairing singly housed monkeys. Implementing short durations of positive reinforcement training across large numbers of singly housed animals may not be the most effective manner for incorporating positive reinforcement training in the behavioural management of laboratory macaques. Rather, directing efforts toward individuals with specific behavioural, management, clinical, research or therapeutic needs may represent a more fruitful approach to improving psychological well-being with this technique.

The stability and uniformity of the following gas mixtures: 90% argon; 85% argon and 15% carbon dioxide (CO2); 70% argon and 30% CO2; 98% nitrogen (N2); 92% N2 and 8% CO2; 90% N2 and 10% CO2; 85% N2 and 15% CO2; 80% N2 and 20% CO2; 70% N2 and 30% CO2; and 90% CO2 by volume in atmospheric air were assessed in a commercial dip-lift stunning system when the cradle was either stationary or in motion. The gas mixtures of 90% argon, 85% argon and 15% CO2, 70% argon and 30% CO2, 85% N2 and 15% CO2, 80% N2 and 20% CO2, 70% N2 and 30% CO2 and 90% CO2 by volume in atmospheric air could be sustained in a commercial dip-lift stunning system. The stability of the gas mixtures 92% N2 and 8% CO2, and 90% N2 and 10% CO2 by volume in atmospheric air were lower than in the previous cases. On the other hand, an N2 concentration higher than 94% by volume in atmospheric air could not be sustained in the stunning system. In addition, gas mixtures of argon and CO2 showed a higher stability than gas mixtures of N2 and CO2. The uniformity at different levels inside the pit (defined as the capacity of the gas to maintain its concentration constant at different levels inside the pit) was higher in 90% argon, or argon and CO2 mixtures and N2 and CO2 mixtures than in 90% CO2. This fact ensures that for the whole time the animals are inside the pit, the same conditions are applied, which is not the case for 90% CO2.

The objective of this study was to assess the aversion to exposure of 90% argon, 70% N2/30% CO2 and 85% N2/15% CO2 by volume in atmospheric air in 24 halothane-free slaughter-weight pigs using aversion learning techniques and behavioural studies in an experimental slaughterhouse. Pigs were subjected to the treatments individually during 2 separate trials of 12 animals each. The time of exposure to the gases was 46 and 32 s, respectively. When the pit contained any of the 3 gas mixtures, the time taken to cross the raceway and enter the cradle (TCREC) increased compared with the training sessions (atmospheric air). The incidence of pigs showing retreat and escape attempts and gasps and the number of times that this behaviour was performed was lower in 90% argon than in the gas mixtures with N2 and CO2. On the other hand, the time to loss of posture was lower with 70% N2/30% CO2 than with argon. The second exposure to all gas mixtures was more aversive than the first and the loss of posture also occurred earlier in the second exposure. In conclusion, pigs showed more aversion to gas mixtures with N2 and either 15% or 30% CO2 by volume than 90% argon by volume.

As animal industry and animal advocacy groups debate how farm animals should be treated, little research has focused on the attitudes of consumers in the United States. This study utilises results of a representative telephone survey to measure consumer attitudes towards farm animal welfare, and investigates how these attitudes vary across individuals. The survey finds that consumers desire high standards of animal care, even if it raises food prices and involves government regulation. Support is particularly strong from females, Democrats, and residents of the Northeastern United States. To provide high standards of animal care, consumers as a whole perceive allowing animals to exhibit natural behaviours and exercise outdoors to be more important than protection from other animals, shelter, socialisation, and comfortable bedding. Consumers vary in their perceptions though, and are divided into three classes: Naturalists, Price Seekers, and Basic Welfarists. Naturalists place great importance on allowing animals to exhibit natural behaviours and exercise outdoors, and comprise 46% of the sample. Price Seekers, comprising 14% of the sample, are primarily concerned with low prices. Basic Welfarists make up 40% of the respondents, and value animal welfare but perceive it can be achieved by simply providing food, water, and treatment for injury and disease. This last group perceives amenities, such as access to outdoors and ability to exhibit natural behaviours, unimportant for the well-being of farm animals.

This study assessed the effect of increasing fibre levels in the concentrate ration on the welfare of sows housed in a large dynamic group. One hundred and twelve Large White * Landrace sows were allocated to one of two treatments over six replicates. Treatments were as follows: (i) High Fibre diet (~15% CF [Crude Fibre]), and (ii) Control diet (~5% CF). Treatments were applied to two separate dynamic groups each containing 33 (±3) sows in a cross-over design, after three replicates the treatments were switched between the groups. Approximately nine sows were replaced in each of these groups at 3-week intervals (each replacement constituting a replicate of the study). Sows on the high fibre diet spent a greater percentage of time lying (High Fibre: 43.8, Control: 28.0, SEM 3.25%), while sows on the control diet spent more time sham chewing (High Fibre: 7.2, Control: 28.8, SEM 1.55%). Sows newly introduced to the group on the high fibre treatment spent proportionally more time in the kennel areas compared to newly introduced sows in the control treatment (High Fibre: 0.893, Control: 0.788, SEM 5.10). In general, aggression occurred at a very low frequency and overall levels did not differ between treatments (High Fibre: 0.005, Control: 0.003, SEM 0.0007 [occurrences per min]). However, sows in the control treatment performed head thrusting (High Fibre: 0.02, Control: 0.00, SEM 0.001 [occurrences per min]), and biting behaviour (High Fibre: 0.02, Control: 0.01, SEM 0.002 [occurrences per min]) more frequently than sows on the high fibre diet. There was no effect of treatment on physiological parameters such as plasma cortisol (High Fibre: 1.34, Control: 1.44, SEM 0.114 ng ml−1) or haptoglobin levels (High Fibre: 0.73, Control: 0.64, SEM 0.080 mg ml−1). In summary, provision of a high fibre diet had a positive effect on the welfare of group-housed dry sows. Sows on the high fibre treatment spent more time resting in the kennel areas, less time performing stereotypic behaviours and showed a reduction in some aggressive behaviours relative to sows fed the control diet.