Introduction
In the last decade or two, research on the consumption of dietary fats and oils has become an important topic. High fat content, together with the type of fat in the diet, has been blamed for causing conditions such as obesity, insulin resistance and metabolic syndrome X. Therefore, life-threatening conditions such as stroke and acute myocardial infarction can be directly related to either the fat content or fat type in the diet(Reference Elson and Quereshi1–Reference Puskas, Nagy and Girics4). However, the inclusion of oils and fats in the diet is necessary, as the lack thereof could lead to other potentially life-shortening disorders, such as glomerulonephritis, hypertension, diabetes mellitus, metabolic syndrome X, psoriasis, Alzheimer's disease, schizophrenia, depression, CHD, atherosclerosis and cancer(Reference Horribon5–Reference Das10). The above-mentioned diseases are not only a result of decreased fat intake, but also due to a shortage of fat-soluble vitamins and essential fatty acids that are normally contained in oils and fats.
Many studies have been done on edible oils and their effects on cardiovascular health. However, few of these studies compared the effects of more than two of these oils with each other. There also seem to be considerable gaps in the literature concerning certain areas of cardiovascular health with respect to many oils. The need to revisit the literature has thus arisen.
Heart disease is still one of the major causes of mortality in the developed world(Reference Banegas, Rodriguez-Artalejo and Graciani11, Reference Jemal, Ward and Hao12). The National Cholesterol Education Program has recommended that a reduction in LDL-cholesterol (LDL) should be used as a treatment of choice for heart disease and that increased LDL-cholesterol be used for the early detection of potential heart disease(13). Previous studies have shown that increased TAG may also be considered to be a risk factor for the development of heart disease(Reference Assman, Schulte and von Eckardstein14–Reference Cullen17). Research has also shown that the attenuation of inflammation may offer protection against ischaemia–reperfusion injury(Reference Mullane, Read and Salmon18–Reference Carnieto, Dourado and Da Luz21). Carnieto et al. (Reference Carnieto, Dourado and Da Luz21) demonstrated in an in vivo dog model that a reduced cyclo-oxygenase-2-related inflammatory response was able to limit tissue damage associated with ischaemia–reperfusion injury. Cyclo-oxygenase-2 is an enzyme that is responsible for converting non-esterified arachidonic acid to pro-inflammatory prostaglandins. These authors conclude that a cyclo-oxygenase-2 inhibitor may offer some protection against a myocardial ischaemia–reperfusion injury.
Apart from their fatty acid content, oils also provide a variety of micronutrients that may have beneficial effects on, amongst others, the cardiovascular system. Both the fatty acid component and micronutrient component of an oil may influence cardiovascular health. Researchers often focus on a particular component of a product and call it the ‘active ingredient’. In the case of natural products, the oil may be viewed as a cocktail of active ingredients that often have a synergistic effect on health(Reference Palozza and Krinsky22–Reference Schroeder, Becker and Skibsted27) (see Table 1).
Table 1 Summary of the content of the oils reviewed
ppm, Parts per million.
* Indicated value is as found in Carotino Premium palm oil (Carotino Sdn Bhd, Johor Bahru, Malaysia).
† Indicated value is as found in Carotino red palm oil concentrate (Carotino Sdn Bhd, Johor Bahru, Malaysia).
Better understanding of the physiological control, mechanisms of lipid metabolism and the associated risk factors for heart disease and stroke has increased markedly via research carried out in recent times. Many of these studies have shown that fatty acids and combinations of fatty acids are beneficial to cardiovascular health(Reference Elson and Quereshi1, Reference Das8, Reference Jones and Lefer20, Reference Carnieto, Dourado and Da Luz21).
The present review will focus on the beneficial effects of dietary edible oils and fat on the cardiovascular system. We will focus on four well-known oils, namely olive oil, sunflower-seed oil (SFO), fish oil and palm oil.
Olive oil
Refining of olive oil
Olive oil is a rich tasty oil produced from the fruit of Olea europaea which grows naturally in the Mediterranean region. This oil is produced from olives by: (1) washing, (2) crushing, (3) kneading and (4) centrifugation of olives(Reference Owen, Mier and Giacosa28, Reference Brenes, Romero and Garciäa29). When the pH of the olive oil is below 3·3, the oil is refined to produce a common olive oil(Reference Owen, Mier and Giacosa28). Refining of olive pomace (a crude oil produced by centrifugation of olives in water) can take place through secondary physical extraction and centrifugation or chemical refining(Reference Brenes, Romero and Garciäa29). Unrefined olive oil is known as virgin olive oil and may be viewed as unique among oils as it is produced without refining. Various grades of olive oil are commercially available, depending on how it was refined. Virgin olive oil should be considered the healthiest, as no refining takes place. This leads to the optimal retention of micronutrients, which are destroyed through chemical refining. Olive oil has a rich medical history and has traditionally been used to treat colic, alopecia, paralysis, rheumatic pain, sciatica and hypertension(Reference Giliani, Khan and Shah30).
Composition of olive oil
Olive oil is a yellow-coloured oil that consists of mainly MUFA, of which oleic acid comprises 72–79 %(Reference Owen, Mier and Giacosa28, Reference Newmark31, Reference Quiles, Ochoa and Ramirez-Tortosa32). In comparison with PUFA, MUFA are less susceptible to oxidation. This in turn leads to increased availability of antioxidants in the active form and better stability of olive oil(Reference Diniz, Cicogna and Padovani3, Reference Owen, Mier and Giacosa28, Reference O'Farrell and Jackson33–Reference Eder, Wacker and Lutz37).
Olive oil also contains some antioxidant micronutrients, namely polyphenols and squalene(Reference Owen, Mier and Giacosa28, Reference Newmark31, Reference Quiles, Ochoa and Ramirez-Tortosa32, Reference Lercker and Rodríguez-Estrada38, Reference Caravaca, Pancorbo and Diaz39). More than 80 % of the olive oil phenolic compounds are lost during the refinery process. Therefore, the phenolic compound content of virgin olive oil (about 230 mg/kg, common range 130–350 mg/kg) is higher than that in common olive oil(Reference Owen, Mier and Giacosa28, Reference Tuck and Hayball40). However, in another study Owen et al. (Reference Owen, Mier and Giacosa28) found that the total phenolic content of olive oil may be as high as 500 mg/kg. The major phenols are tyrosol, hydroxytyrosol, oleuropein and ligstroside(Reference Owen, Mier and Giacosa28, Reference Caravaca, Pancorbo and Diaz39, Reference Perona, Cabello-Moruno and Ruiz-Gutierrez41). Of these polyphenols, tyrosol and hydroxytyrosol are the most abundant and represent 30 % of the total polyphenol content of olive oil(Reference Owen, Mier and Giacosa28). Formation of tyrosol and hydroxytyrosol takes place through the hydrolysis of oleuropein and ligstroside during storage of the olive oil(Reference Romero, Medina and Vargas42). Tyrosol, hydroxytyrosol and oleuropein all contain a catechol group, which has been shown to have antioxidant activity(Reference Owen, Mier and Giacosa28, Reference Caravaca, Pancorbo and Diaz39, Reference Montedoro, Servilli and Baldioli43, Reference Visioli, Poli and Gall44). Research has shown that hydroxytyrosol and oleuropein are the most effective antioxidants in olive oil and may exert better antioxidant activity than vitamin E(Reference Owen, Mier and Giacosa28, Reference Perona, Cabello-Moruno and Ruiz-Gutierrez41).
Olive oil is considered to be extremely rich in squalene and contains approximately 0·7 % of this hydrocarbon(Reference Owen, Mier and Giacosa28, Reference Newmark31). Other foods and oils may contain squalene at a level between 0·002 and 0·03 %. Squalene is an antioxidant by virtue of its ability to quench singlet oxygen radicals, and inhibits cholesterol synthesis through 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibition(Reference Newmark31, Reference Relas, Gylling and Miettinen45). Apart from the inhibition of HMG-CoA reductase by olive oil, its fatty acid composition may play a role in improving the serum lipid profile. This, however, has not been proven conclusively(Reference Heyden46).
Other micronutrients within olive oil include lignans, phenyl-ethyl alcohols and secoiridoids(Reference Carrasco-Pancorbo, Cerretani and Bendini47). These micronutrients have also been credited with antioxidant effects, but not as significant as oleuropein and hydroxytyrosol(Reference Carrasco-Pancorbo, Cerretani and Bendini47).
Cardiovascular studies with olive oil
A study by Keys et al. (Reference Keys, Menotti and Karvonen48) suggested that olive oil consumption decreases the incidence of degenerative diseases such as CHD and cancer. This study used surveys to measure the general diet of a population, and calculate the fat content of the diet. The mortality rate of men between the ages of 40 and 59 years was then monitored over a 15-year period for these populations. One of the major findings of this study was a negative correlation between MUFA in the diet and death due to CHD.
Antioxidant effects of olive oil
The high content of polyphenols in olive oil suggests that they can be important antioxidants in vivo, although no conclusions can be made as to their effectiveness. One study showed that even when these polyphenol levels were increased in the blood, they failed to protect LDL from oxidation(Reference Vissers, Zock and Wiseman49). Volunteers in this study consumed both a high- and low-polyphenol diet, respectively, for 3 weeks, with a 2-week washout period between the two diets. The high-polyphenol diet contained 308 mg polyphenols/kg, while the low-polyphenol diet contained 43 mg/kg. The mean difference in polyphenol intake between the diets was 18 mg/d. It is possible that the difference in polyphenol content of the diets was too small to achieve significant protection against HDL and LDL oxidation. However, the authors designed the high-polyphenol diet to contain the highest amount of polyphenols that would be possible to consume in a daily diet. A study by Eder et al. (Reference Eder, Wacker and Lutz37) investigated the effect of supplementation of edible oils on lipid peroxidation in the liver. Female rats were fed a standard rat chow diet, or a standard rat chow diet plus SFO, rapeseed oil, olive oil or coconut oil for 4 weeks. They found that olive oil neither contributes to lipid peroxidation, nor prevents it and concluded that there is no antioxidative protection offered by olive oil supplementation. However, chemical analysis by Briante et al. (Reference Briante, Febbraio and Nucci50) has shown that polyphenols present in olive oil may have an antioxidant or pro-oxidant effect. They concluded, first, that low concentrations of olive oil polyphenols (lower than 23 μg oleuropein/mg and 18·2 μg hydroxytyrosol/mg) have a pro-oxidant effect on LDL, while higher concentrations have an antioxidant effect. Second, they found that metal ions play a major role in LDL oxidation in vivo, and that olive oil polyphenols may reduce Cu ions and thus prevent LDL oxidation. In a study by Aguilera et al. (Reference Aguilera, Mesa and Ramirez-Tortosa51), virgin olive oil was compared with α-tocopherol-enriched SFO, and was found to offer better protection against LDL oxidation. Male volunteers with peripheral vascular disease were fed a diet supplemented with olive oil or α-tocopherol-enriched SFO for 4 months. In this study the antioxidant content in the blood of the olive oil-supplemented group was found to be lower than that of the SFO group. However, olive oil still offered better protection against oxidative stress, as shown by LDL oxidation. The authors of this study suggested that the high levels of antioxidants in the SFO group could not protect LDL from oxidation due to the oil's high level of unsaturated fat. The results of this study may alternatively argue that the polyphenols in olive oil were more protective than the added α-tocopherol in SFO. The fact that the antioxidant content in the blood was also lower in the olive oil-fed group compared with that in the SFO group may suggest that more antioxidants are not necessarily better. These results may thus argue that natural oils are better than artificially manipulated oils.
Hydroxytyrosol and oleuropein have been shown to have a dose-dependent effect by which they can inhibit LDL oxidation(Reference Owen, Mier and Giacosa28, Reference Visioli, Poli and Gall44, Reference Aguilera, Mesa and Ramirez-Tortosa51). The study by Owen et al. (Reference Owen, Mier and Giacosa28) showed these molecules to be more effective antioxidants than vitamin E, dimethyl sulfoxide and butylated hydroxytoluene. Rietjens et al. (Reference Rietjens, Bast and Haenen52) argued that hydroxytyrosol offers effective protection against LDL oxidation in vivo and in vitro, but that ex vivo experiments lead to a false-negative result. This false-negative result is achieved by the removal of hydroxytyrosol from LDL during the isolation of the LDL and the subsequent oxidation thereof. Furthermore, hydroxytyrosol has also been shown to reduce cyclo-oxygenase and also reduces platelet aggregation(Reference Visioli, Poli and Gall44, Reference Fabiani, De Bartolomeo and Rosignoli53). Both these effects are associated with a decreased inflammatory response.
It is generally accepted that olive oil decreases oxidative stress(Reference Diniz, Cicogna and Padovani3, Reference Quiles, Ochoa and Ramirez-Tortosa32, Reference Theriault, Chao and Wang54, Reference Kritchevsky55). Quiles et al. (Reference Quiles, Ochoa and Ramirez-Tortosa32) compared olive oil with SFO and its ability to reduce the amount of DNA double-strand breakage. In this study rats were fed for 6 and 24 months, respectively, with diets containing either olive oil or SFO as a fat source. Some rats continued on this diet after 24 months, in order to determine the mean survival rate on each diet. The beneficial effects of MUFA in olive oil are clear, as it contributes to an increased lifespan of rats and a decrease in reactive oxygen species (ROS)-associated DNA damage. Furthermore, higher concentrations of plasma retinol and coenzyme Q10 were found to be present in the olive oil-treated group when compared with a SFO group. This suggests that there was less oxidative stress in rats supplemented with olive oil than in those supplemented with SFO. Fabiani et al. (Reference Fabiani, Rosignoli and De Bartolomeo56) showed that an extract consisting of a mixture of olive oil antioxidants was able to protect peripheral blood mononuclear cells and promyelocytic leukaemia cells from H2O2-induced DNA damage. Cell cultures were incubated with various concentrations of antioxidants and 40 μm-H2O2 for 30 min. Antioxidant doses as low as 1 μmol/l were still able to offer significant protection against DNA damage, as measured by the Comet assay.
A study by Briante et al. (Reference Briante, Febbraio and Nucci50) showed that olive leaf extracts added to olive oil increased the efficacy of the oil's antioxidant effect. This study showed that oleuropein (derived from olive leaf extract) and hydroxytyrosol worked synergistically to prevent LDL oxidation and thus atherosclerosis. This corresponds with the comment by Stahl et al. (Reference Stahl, Junghans and De Boer24). The addition of these polyphenols to SFO improved the immune status when supplemented to the diet of volunteers for 8 weeks(Reference Baeza, De Castro and Díaz57, Reference Díaz, Gómez and Nova58). Baeza et al. (Reference Baeza, De Castro and Díaz57) showed that leucocytes had increased glutathione peroxidase activity after 8 weeks of supplementation, while in the study by Díaz et al. (Reference Díaz, Gómez and Nova58) no significant changes were seen after 3 weeks of enriched oil supplementation. The authors suggested that further studies should be done to determine an optimal supplementation time.
Effects of olive oil on serum lipids
Studies done by Demonty et al. (Reference Demonty, Chan and Pelled59) report on hypercholesterolaemic subjects whose diets were supplemented with fish oil, olive oil or enriched oils for 4 weeks. Olive oil supplementation was found to significantly lower LDL concentrations when compared with fish oil supplementation and also led to higher levels of serum TAG than fish oil supplementation, whereas fish oil enriched with plant sterols led to the lowest total cholesterol, LDL and TAG levels. Although olive oil showed a hypocholesterolaemic effect on the serum lipid profile, there is some evidence to suggest that it does not significantly affect the phospholipid profile of the heart or erythrocyte(Reference Metcalf, James and Gibson60). These authors compared the changes in atrium and erythrocyte fatty acid composition of volunteers after different periods of olive oil, flaxseed oil and fish oil supplementation. The olive oil group showed no significant difference from the control group in either the erythrocyte or atrium fatty acid composition. Heyden(Reference Heyden46) concluded that MUFA do not play a significant role in reducing cholesterol or LDL-cholesterol. However, squalene present in olive oil has been shown to down-regulate HMG-CoA reductase activity and therefore reduce cholesterol synthesis(Reference Newmark31, Reference Relas, Gylling and Miettinen45).
Effects of olive oil on blood pressure
Some studies have shown that olive oil has a beneficial effect on blood pressure. Giliani et al. (Reference Giliani, Khan and Shah30) found that intravenous administration of olive oil extracts reduced both systolic and diastolic blood pressure in normotensive rats. Ferrara et al. (Reference Ferrara, Raimondi and d'Episcopo61) showed that patients on an olive oil-supplemented diet were able to reduce their antihypertensive medication, in contrast to patients on a SFO-supplemented diet. Giliani et al. (Reference Giliani, Khan and Shah30) suggested that olive oil may be a Ca channel agonist and thereby reduce systolic and diastolic blood pressure. Others have suggested that olive oil improve endothelial function by inhibiting the formation of ROS, leading to a NO-mediated vasorelaxation(Reference Perona, Cabello-Moruno and Ruiz-Gutierrez41, Reference Ruiz-Gutierrez, Muriana and Guerrero62, Reference Alonso, Ruiz-Gutierrez and Martinez-Gonzalez63). Other authors have suggested decreased vascular tone and changes in the fatty acid composition of the aorta as a possible mechanism(Reference Ferrara, Raimondi and d'Episcopo61).
Olive oil in the ischaemia–reperfusion model
To our knowledge, little is known about the effects of dietary olive oil supplementation on cardiac ischaemia and reperfusion. Manna et al. (Reference Manna, Migliardi and Golino64) performed a study to investigate the effectiveness of oleuropein in offering protection against oxidative stress associated with global myocardial ischaemia and reperfusion. Rat hearts were isolated and perfused with a Langendorff perfusion apparatus. Control hearts were stabilised for 20 min, after which they were subjected to 30 min of normothermic global ischaemia and 60 min of reperfusion. Experimental hearts were perfused with oleuropein at a concentration of 50 μmol/l for 15 min before the induction of ischaemia. Perfusion with oleuropein was able to significantly reduce creatinine kinase release during reperfusion, suggesting that it offered protection against tissue damage. Decreased glutathione and reduced glutathione released in coronary effluent, along with the decreased thiobarbituric acid-reactive substances in heart tissue, indicate that oxidative stress was significantly decreased by oleuropein. Further studies need to be done to confirm cardioprotection by dietary olive oil supplementation and on cardiac health in general.
Benefits of olive oil supplementation
Even though olive oil contains micronutrients such as polyphenols and squalene, the MUFA in the oil have been thought to be the major active component. MUFA are known to have a beneficial effect on the serum lipid profile and thus decrease the risk of CVD(13, Reference Heyden46, Reference Demonty, Chan and Pelled59, Reference Metcalf, James and Gibson60). Furthermore, these fatty acids are stable in oxidative stress conditions and are less likely to react with ROS when compared with PUFA(Reference Diniz, Cicogna and Padovani3, Reference Owen, Mier and Giacosa28). MUFA may thus be seen as beneficial to cardiovascular health, as they does not cause negative serum lipid profiles, which are normally associated with SFA. Furthermore, MUFA do not increase oxidative stress by the formation of lipid hydroperoxides, as is the case with PUFA(Reference Diniz, Cicogna and Padovani3, Reference Quiles, Ochoa and Ramirez-Tortosa32, Reference Newaz, Yousefipour and Nawal35–Reference Eder, Wacker and Lutz37, Reference Theriault, Chao and Wang54, Reference Kritchevsky55).
Olive oil polyphenols have an antioxidative effect that inhibits LDL oxidation and thus reduces atherosclerosis(Reference Owen, Mier and Giacosa28, Reference Perona, Cabello-Moruno and Ruiz-Gutierrez41, Reference Manna, Migliardi and Golino64). Squalene also exerts an antioxidant effect and inhibits cholesterol synthesis(Reference Newmark31, Reference Relas, Gylling and Miettinen45). Together these micronutrients may affect atherosclerosis by the inhibition of LDL oxidation and reduction of total cholesterol(Reference Owen, Mier and Giacosa28, Reference Visioli, Poli and Gall44, Reference Fabiani, Rosignoli and De Bartolomeo56, Reference Manna, Migliardi and Golino64, Reference Patrick and Uzick65). Hydroxytyrosol also reduces inflammation by the reduction of pro-inflammatory cyclo-oxygenase and platelet aggregation(Reference Visioli, Poli and Gall44, Reference Fabiani, De Bartolomeo and Rosignoli53).
A number of studies have shown that olive oil supplementation decreases hypertension in human subjects through mechanisms not yet resolved(Reference Giliani, Khan and Shah30, Reference Keys, Menotti and Karvonen48, Reference Ferrara, Raimondi and d'Episcopo61, Reference Manna, Migliardi and Golino64, Reference Patrick and Uzick65). Reduction of hypertension has been shown in human studies and animal models with varying supplementation times, or modes of application. In many studies this was not the main aim of the study but rather an additional observation noted.
It is evident that olive oil, due to its micronutrient content and fatty acid composition, can play a vital role in maintaining beneficial serum lipid profiles. Together with its ability to reduce systemic oxidative stress, blood pressure and inflammation, it becomes an appropriate dietary supplement for lowering the risk of CHD.
Sunflower-seed oil
Refining of sunflower-seed oil
SFO is produced by refining the seeds of the sunflower. The refinery process includes the following steps: (1) pressing to yield crude oil; (2) acidification and neutralisation; (3) pre-gumming by centrifugation; (4) washing; 5) bleaching; (6) gumming by filtration; and (7) deodorisation(Reference Zitouni, Errahali and Metche66).
Components of sunflower-seed oil
This refinery process yields a yellow oil, rich in PUFA, of which the major PUFA is linoleic acid (60–70 %). Oleic acid and stearic acid are the major MUFA and SFA, respectively. The levels of MUFA and SFA present in SFO may vary, depending on the desired product being manufactured(Reference Eder, Wacker and Lutz37, Reference Aguilera, Mesa and Ramirez-Tortosa51, Reference Navarro, Hortelano and Periago67–Reference AbuGhazaleh, Felton and Ibrahim69). Commercially there are three main forms of SFO available. The first is a high-PUFA SFO which consists of up to 75 % PUFA. The second form is a high-MUFA SFO which contains up to 45 % MUFA, whilst the third is a high-stearic acid SFO which may contain up to 14 % stearic acid. These three forms of SFO have very different effects on the serum lipid profile. The most commonly used form of SFO is the high-PUFA SFO. Whilst the high-stearic acid SFO is mainly used in industrial processes, high-MUFA SFO is used for general cooking. SFO contains some natural vitamin E in the form of tocopherol(Reference Eder, Wacker and Lutz37, Reference Aguilera, Mesa and Ramirez-Tortosa51, Reference AbuGhazaleh, Felton and Ibrahim69). Most of the natural tocopherol in SFO is destroyed by refining. It is therefore often artificially supplemented with tocopherol.
n-6 PUFA provided by SFO in the diet may play a vital role in the regulation of inflammation, as it is used to produce pro-inflammatory prostaglandins(Reference Navarro, Hortelano and Periago67, Reference Simopoulos70, Reference Das, Lekli and Das71).
Cardiovascular studies with sunflower-seed oil
Only a few studies have been done with SFO to determine its influence on cardiovascular health. SFO has been used as a control in many studies when compared with other oils(Reference Quiles, Ochoa and Ramirez-Tortosa32, Reference Aguilera, Mesa and Ramirez-Tortosa51, Reference Larsen, Jespersen and Marckmann68, Reference Charnock, Sundram and Abeywardena72, Reference De Roos, Geelen and Ross73). The choice of SFO as a control may be due to the fact that SFO has shown few effects on the cardiovascular system apart from its beneficial effect on the serum lipid profile(Reference Quiles, Ochoa and Ramirez-Tortosa32, Reference Aguilera, Mesa and Ramirez-Tortosa51, Reference Larsen, Jespersen and Marckmann68, Reference Charnock, Sundram and Abeywardena72, Reference De Roos, Geelen and Ross73). SFO had little effect on myocardial arrhythmia after 44 weeks of 12 % supplementation in the diet of rats(Reference Charnock, Sundram and Abeywardena72). SFO supplementation (19 % of total diet) could also not induce an anti-thrombotic effect after being supplemented for 22 d to volunteers(Reference Larsen, Jespersen and Marckmann68). Aguilera et al. (Reference Aguilera, Mesa and Ramirez-Tortosa51) found little protection against oxidative stress after 4 months of supplementation with SFO. This was supported by similar findings made by Quiles et al. (Reference Quiles, Ochoa and Ramirez-Tortosa32) after 6 and 24 months of SFO supplementation (as the sole fat supply) in the diet of rats. Little effect was seen on acute-phase inflammatory markers and apolipoprotein levels after 12 weeks of 3·5 g SFO supplementation(Reference De Roos, Geelen and Ross73).
Effects of sunflower-seed oil on serum lipids
It has been shown that SFO can modulate the serum lipid profile(Reference Girardet, Jacotot and Mendy74–Reference Lambert, Goedecke and Bluett76) and the major finding was shown to be a decrease in total cholesterol and LDL-cholesterol. Lambert et al. (Reference Lambert, Goedecke and Bluett76) supplemented the diets of regularly exercising individuals with 3·90 g high-MUFA SFO for 12 weeks. A reduction in total cholesterol and LDL-cholesterol of male and female volunteers was found after the supplementation period (men: total cholesterol decreased 6·4 % while LDL-cholesterol decreased 11·1 %; women: total cholesterol decreased 12·8 % while LDL-cholesterol decreased 8·33 %). There was also a significant reduction of 12·5 % in HDL-cholesterol in women after the 12-week supplementation period. The decreased HDL-cholesterol was ascribed to weight loss or increased dietary consciousness of the participants. Similar findings were made by Nydahl et al. (Reference Nydahl, Gustafsson and Ohrvall75) when they fed a SFO-supplemented diet to 101 volunteers for 3 weeks. During the supplementation period all other oils in the diet were replaced with SFO. A decrease of 4 % in total cholesterol, 5–7 % in LDL-cholesterol and 5 % in apo B was observed after the 3 weeks. There was virtually no difference in other molecules of the serum lipid profile. Girardet et al. (Reference Girardet, Jacotot and Mendy74) showed a decrease in total serum cholesterol, cholesteryl esters and TAG in rats fed a 12 % SFO-supplemented diet for 1 year. However, in a study done by Aguilera et al. (Reference Aguilera, Mesa and Ramirez-Tortosa51), 4 months of dietary supplementation with high-PUFA SFO and virgin olive oil in patients with peripheral vascular disease, SFO supplementation did not affect the serum lipid profile significantly. In this study all meals were cooked in SFO and patients received additional supplements (15·14 g of the oil per d). The fat content of the diet in this study may have been excessive, as supplementation with virgin olive oil in this study led to similar results. This pattern was also seen in a study by Quiles et al. (Reference Quiles, Ochoa and Ramirez-Tortosa32) where all the fat in the diet was replaced by SFO for 6–24 months.
Sunflower-seed oil as an antioxidant or pro-oxidant
Due to the relative lack of micronutrients in SFO, hydroxytyrosol is added to commercial blends in some instances to increase the antioxidant content. Hydroxytyrosol is a polyphenol which in this case has been shown to improve immunity by increasing leucocyte glutathione peroxidase activity(Reference Baeza, De Castro and Díaz57, Reference Díaz, Gómez and Nova58).
Having large amounts of PUFA in the diet may be worse than large amounts of SFA, even despite its positive impact on the serum lipid profile. A study by Diniz et al. (Reference Diniz, Cicogna and Padovani3) showed that a 5-week diet containing 47·0 g linoleic acid (the major n-6 PUFA in most edible oils) per 100 g fat in the diet leads to higher oxidative stress than a diet containing 81·7 g saturated fat per 100 g fat in the diet. In this study, the high PUFA group showed significantly increased tissue hydroperoxide and lipoperoxide concentrations when compared with a high SFA group and the control group. This increase in ROS was accompanied by a significant decrease in superoxide dismutase-, catalase- and citrate synthase activity when compared with a high SFA group and the control group. The high-PUFA diet also contained significantly less glycogen than the other diet groups. Diniz et al. (Reference Diniz, Cicogna and Padovani3) suggest that the increased susceptibility to lipoperoxidation and metabolic shifting is associated with high levels of PUFA in the diet. These negative effects of PUFA supplementation may be so detrimental that they outweigh the positive effects that PUFA have on the serum lipid profile. In another study it was found that SFO supplementation led to statistically significant higher adduct (DNA double-strand breakage) levels in the kidneys, lungs, glandular mucosa, small intestine mucosa and colon mucosa of rats(Reference Eder, Wacker and Wanek77). DNA adduct levels were measured after a 4-week diet containing 169 g SFO, rapeseed oil, coconut oil or olive oil per kg diet. Eder et al. (Reference Eder, Wacker and Wanek77) suggested that the increased DNA adduct levels in dietary SFO-supplemented rats may be associated with an increased genotoxic cancer risk. They concluded that the vitamin E content of the SFO evidently did not significantly contribute to a reduction in DNA adduct levels. It would normally be expected that vitamin E reduces DNA adduct levels, as DNA adducts are the product of lipid peroxidation. However, they speculated that the high linoleic content in SFO had a higher lipid peroxidation-inducing effect than the inhibitory effect of the vitamin E contained in the oil. In another study, these authors also postulated that the vitamin E content of SFO is not high enough to compensate for the high concentrations of n-6 PUFA in this oil(Reference Eder, Wacker and Lutz37). In this study a similar supplementation was used to that of Eder et al. (Reference Eder, Wacker and Wanek77). However, the focus of this study was to determine the effects of the oils on lipid peroxidation in the liver. Aguilera et al. (Reference Aguilera, Mesa and Ramirez-Tortosa51) showed that 4 months of dietary SFO supplementation in patients with peripheral vascular disease could not protect LDL from oxidation. The SFO used in this study was enriched with tocopherol (1200 mg/kg in total). Despite the increased antioxidants added to the oil, thiobarbituric acid-reactive substances were still found to be significantly higher in the blood of subjects receiving SFO than in those receiving unaltered olive oil. These results argue that the unsaturated fatty acids in SFO lead to high levels of oxidative stress, despite the high levels of antioxidants present in the oil. It can also be concluded that artificial addition of antioxidants to oils may not yield desirable results.
Sunflower-seed oil in the ischaemia–reperfusion model
To our knowledge, no studies have been done to determine whether SFO supplementation protects against ischaemia–reperfusion injury. Judging from studies with SFO in other experimental models, the effects on reperfusion recovery would be minimal(Reference Quiles, Ochoa and Ramirez-Tortosa32, Reference Aguilera, Mesa and Ramirez-Tortosa51, Reference Larsen, Jespersen and Marckmann68, Reference Charnock, Sundram and Abeywardena72, Reference De Roos, Geelen and Ross73).
Benefits of sunflower-seed oil supplementation
Despite modulation of the serum lipid profile and the provision of essential fatty acids which may help in the regulation of inflammation, SFO has a neutral effect on the cardiovascular system. This makes SFO an excellent choice as a control oil in cardiovascular research(Reference Quiles, Ochoa and Ramirez-Tortosa32, Reference Aguilera, Mesa and Ramirez-Tortosa51, Reference Larsen, Jespersen and Marckmann68, Reference Charnock, Sundram and Abeywardena72, Reference De Roos, Geelen and Ross73). The beneficial effect of SFO in ischaemia–reperfusion injury can only be clarified with additional studies.
Due to the positive effects on the serum lipid profile, this oil may lead to a decrease in atherosclerosis and therefore should be considered to be beneficial to cardiovascular health(Reference Girardet, Jacotot and Mendy74–Reference Lambert, Goedecke and Bluett76). In contrast, however, if SFO is not used sparingly in the diet, it may lead to pro-oxidant effects and thus increase susceptibility to cardiovascular disorders.
Fish oil
Refining of fish oil
Fish oil is derived from the tissues of oily fish. There is very little refining or chemical alteration that takes place after the recovery of the oil from these fish species(Reference Moghadasian78). Fish oil supplementation in the diet is known to offer protection against various pathological conditions, such as CVD, respiratory diseases, diabetes, depression, cancers, inflammatory and immune renal disorders(Reference Simopoulos70, Reference De Caterina, Endres and Kristensen79, Reference Priyamvada, Priyadarshini and Arivarasu80). After production of fish oil, antioxidants are often added in order to improve storage time. Fish oil also often undergoes microencapsulation before storage in order to improve shelf life and improve the palatability of the product.
Components of fish oil
Fish is known to be a good source of protein, vitamin B12, vitamin D, Se, iodine and long-chain n-3 fatty acids. The n-3 fatty acids supplied by fish oil are mostly EPA (20: 5) and DHA (22: 6)(Reference Metcalf, James and Gibson60, Reference Dahl, Bjorkkjaer and Graff81, Reference Conde, Cyrino and Bottino82). In the past many studies focused on the effects of EPA only, as it is provided by fish oil in large amounts. However, recently the focus has shifted to the beneficial effects of DHA on health(Reference Conde, Cyrino and Bottino82–Reference Theobald, Goodall and Sattar84). A study by McLennan et al. (Reference McLennan, Howe and Abeywardena85) suggests that DHA may be responsible for most of the beneficial cardiovascular effects of dietary fish oil supplementation. In this study, spontaneously hypertensive rats were fed either DHA or EPA or both in low doses (0·4–1 % of energy intake). DHA proved to be more effective than EPA in reducing ischaemia-induced myocardial arrhythmias, hypertension, thromboxane-like vasoconstrictor responses (in the aorta) and the development of proteinuria after salt loading of the diet in the spontaneously hypertensive rats. The question was subsequently raised whether it is not DHA that may be the principal active component of fish oil in offering cardiovascular protection.
It is of great importance to include sufficient amounts of EPA and DHA in the diet, as the human body can only convert small amounts of α-linolenic acid (18: 3) to EPA (5 %) and DHA (less than 5 %)(Reference Plourde and Cunnane86, Reference Lee, O'Keefe and Lavie87). It has been shown that fish oil supplementation is able to significantly increase myocardial levels of DHA and EPA in 1 week of supplementation(Reference Metcalf, James and Gibson60). Volunteers in this study received 10 ml fish oil concentrate per d for either 7, 14 or 21 d. Control groups in this study received either flaxseed oil, olive oil, or no supplementation. Therefore, the increase in DHA and EPA could play a vital role in preventing life-threatening arrhythmias in supplemented individuals. Furthermore, the increase in DHA and EPA levels in the myocardium was found to be inversely proportional to arachidonic acid levels when fish oil was supplemented in the diet. This indicates that arachidonic acid is replaced by EPA and DHA in cell membranes.
Cardiovascular studies with fish oil
Effects of fish oil on blood pressure
McLennan et al. (Reference McLennan, Howe and Abeywardena85) showed that dietary supplementation with purified DHA (3·9–10 % of daily energy intake) delayed the onset of hypertension in spontaneously hypertensive rats, while EPA supplementation could not render similar results. They also showed that DHA can inhibit thromboxane-like vasoconstrictor responses in spontaneously hypertensive rat aortas. Furthermore, DHA supplementation delayed the onset of salt loading-induced proteinuria in these spontaneously hypertensive rats with already established hypertension. However, dietary supplementation with EPA did not show similar results in this study. Another study showed that fish oil supplementation was able to decrease diastolic blood pressure by the provision of DHA(Reference Theobald, Goodall and Sattar84). Purified DHA was tested against a placebo control group in this study and exhibited a decreased diastolic blood pressure. Healthy volunteers were recruited and supplemented with capsules containing 500 mg of either DHA or olive oil for at least 3 months. The antioxidant levels in the two capsules were adjusted to similar levels. Together with a decreased blood pressure, it was also shown that a decrease in heart rate occurred and it was speculated that the decrease in blood pressure was caused by the decreased heart rate rather than a decrease in arterial stiffness.
Effects of fish oil on inflammation
In a study on hypercholesterolaemic mice, Chiu et al. (Reference Chiu, Wang and Chien88) showed that fish oil supplementation decreased adhesion molecule expression and some inflammatory markers in early-stage sepsis. Fish oil supplementation was performed in this study over a 3-week period, following a high-fat diet. Late-stage inflammatory markers were not affected and myeloperoxidase activity at the sepsis site was similar in an olive oil-fed group. Chiu et al. (Reference Chiu, Wang and Chien88) concluded that even though fish oil decreases adhesion molecule expression and early-stage inflammatory markers when fed to hypercholesterolaemic mice, it did not cause immunosuppression when sepsis was induced in these animals. As inflammation plays an important role in the pathophysiology of myocardial infarction, reduction in inflammatory markers may prevent the development of heart disease(Reference Mullane, Read and Salmon18–Reference Carnieto, Dourado and Da Luz21).
Effects of fish oil on cardiac arrhythmia
Charnock et al. (Reference Charnock, Sundram and Abeywardena72) showed that fish oil mixed in a 1:1 ratio with sheep fat significantly reduced cardiac arrhythmias during coronary occlusion and reperfusion, when compared with rats fed sheep fat only. Diets of rats were supplemented with 12 % of either sheep fat, sheep fat–fish oil blend, physically refined palm oil, chemically refined palm oil or SFO for 12 months. In this study, neither palm oil nor SFO could decrease arrhythmias significantly when compared with the sheep fat-fed control group. In another study it was shown that fish oil was more effective in reducing cardiac arrhythmia during or after ischaemia when compared with olive oil and sheep fat and to a lesser extent SFO(Reference McLennan89). In this study, 30-week-old rats received 12 % olive oil, SFO, fish oil or sheep fat supplementation for 12 weeks. SFO and fish oil supplementation led to significantly lower arrhythmia scores during and after ischaemia when compared with sheep fat. Fish oil, however, led to a much lower incidence of arrhythmia than SFO. These results suggest that PUFA do not only decrease arrhythmia by replacing SFA in the membrane, but that they play an active role in reducing arrhythmia. If it is accepted that PUFA reduce arrhythmia only by the replacement of SFA in the membrane, one would expect that olive oil supplementation would also be able to reduce arrhythmia. However, olive oil was not shown to be able to reduce arrhythmia in this study and therefore the authors suggested another mechanism of protection by PUFA. Furthermore, these results also indicate that n-3 PUFA are more effective in reducing arrhythmia than n-6 PUFA. It can therefore be accepted that an unknown mechanism of protection can be associated with n-3 PUFA but not n-6 PUFA. McLennan et al. (Reference McLennan, Howe and Abeywardena85) showed that low doses of purified DHA intake (0·4–1·1 % of daily energy intake) could reduce post-ischaemic cardiac arrhythmias in spontaneously hypertensive rats. Dietary supplementation with a similar dosage of EPA did not have the same effect as DHA. McLennan & Abeywardena(Reference Mclennan and Abeywardena90) concluded that fish oil is a powerful modulator of arrhythmia. These authors ascribe the ability of fish oil to decrease arrhythmias, especially ventricular fibrillation, to the incorporation of long-chain PUFA (especially DHA) into the myocardium. They suggest that DHA is the main long-chain PUFA responsible for fish oil cardioprotection, as it is selectively incorporated into myocardial cell membranes. Several possible mechanisms have been suggested for the anti-arrhythmic action of long-chain PUFA. These include altered sympathetic innervation to the conduction system and coronary vessels, increased fluidity of the lipid environment of cardiac membranes, different temperature–activity relationships of key membrane enzymes and altered handling of intracellular Ca2+(Reference Nielsen and Owman91–Reference Wang, Schulze and Suarez-Pinzon93). Abdukeyum et al. (Reference Abdukeyum, Owen and McLennan94) compared ischaemic preconditioning and several edible oils in their ability to exert anti-arrhythmogenic effects during myocardial ischaemia and reperfusion in an isolated perfused rat heart model. Rats of the fish oil group received 10 % fat (of which 7 % was tuna fish oil) in their diet for 6 weeks. These authors found that the anti-arrhythmogenic effects of fish oil supplementation were comparable with that of ischaemic preconditioning. A study by Hlavackova et al. (Reference Hlavackova, Neckar and Jezkova95) demonstrated significant decreases in ischaemia–reperfusion-induced arrhythmia in rats fed an n-3-rich diet when compared with rats fed a SFA- or n-6-rich diet. After 10 weeks of supplementation, one group of each diet was exposed to hyperbaric conditions for 5–6 weeks, after which hearts were perfused. Pre-ischaemic hypoxic incidents were shown to increase resistance to ischaemia–reperfusion-induced arrhythmia in the n-3-fed group.
Other studies suggest that fish oil or n-3 PUFA supplementation does not always offer protection against cardiac arrhythmias. In patients with an implantable cardioverter defibrillator, fish oil supplementation was found to exhibit inconsistent effects. In some cases it was anti-arrhythmic, in others pro-arrhythmic, whilst in some it had no effect(Reference Burr, Ashfield-Watt and Dunstan96–Reference Wilhelm, Asskali and Kraehner100). Burr et al. (Reference Burr, Ashfield-Watt and Dunstan96) found that n-3 PUFA supplementation led to increased mortality and sudden cardiac death in patients with angina pectoris. Men with chest pain were asked to supplement their diet with two portions of oily fish or 3 g fish oil per week for 6 months. The control group in this study did not modify their diet. The amount of cardiac and sudden arrhythmic deaths recorded in the fish oil group was significantly higher than that in the control group. The authors could not explain these findings. Den Ruijter et al. (Reference Den Ruijter, Berecki and Opthof99) demonstrated that n-3 PUFA have different electrophysiological effects when they are incorporated into the membranes than when they are in the blood. These authors speculated that this difference in electrophysiological effects may play a role in the seemingly contradictory effects of n-3 PUFA supplementation on cardiac arrhythmia. Results of a study by Wilhelm et al. (Reference Wilhelm, Asskali and Kraehner100) are in agreement with the aforementioned speculation. This study focused on patients with structural heart disease, as most sudden deaths are caused by ventricular arrhythmias in patients with structural heart disease or impaired left ventricular function(Reference Huikuri, Castellanos and Meyerburg101). Wilhelm et al. (Reference Wilhelm, Asskali and Kraehner100) found that patients with heart failure or structural heart disease showed altered erythrocyte fatty acid profiles. Their results suggest that higher levels of n-3 PUFA in erythrocytes may have caused increased cardiac arrhythmia. This in turn suggests that erythrocyte n-3 PUFA levels may be seen as an independent risk factor for occurring cardiac arrhythmias.
Effects of fish oil on serum lipids
In a study by Demonty et al. (Reference Demonty, Chan and Pelled59) it was shown that supplementing 7·6 g fish oil per d for 29 d was able to decrease serum TAG when compared with olive oil supplementation in hypercholesterolaemic volunteers. This study also demonstrated that plant sterols esterified to fish oil are even more effective in reducing TAG. Plant sterol esters were also able to decrease LDL-cholesterol levels when compared with an olive oil-supplemented control group. Fish oil has beneficial effects on the serum lipid profile. It is known to reduce cholesterol and TAG. Reducing TAG is of great importance to diabetics or metabolic syndrome-X patients, as the increased NEFA associated with these conditions may interfere with cardiac function(Reference D'Allesandro, Chicco and Lombardo102). A study done by D'Allesandro et al. (Reference D'Allesandro, Chicco and Lombardo102) used a sucrose-rich diet to induce lipotoxicity in rats after 8 months of feeding. Changing the fat source in the diet from maize oil to fish oil for the last 2 months of feeding was able to reduce lipotoxicity. Another study showed that n-3 PUFA or fish supplementation may reduce subclinical atherosclerosis(Reference He, Liu and Daviglus103). These authors used coronary artery Ca score, common carotid intima-media thickness, internal carotid intima-media thickness and ankle brachial index to measure the level of atherosclerosis in 5488 individuals after evaluating their diets by questionnaire. They concluded that dietary supplementation with n-3 PUFA or fish reduced the incidence of subclinical atherosclerosis.
Fish oil in the ischaemia–reperfusion model
While many studies focused on the effects of fish oil on myocardial arrhythmias caused by ischaemia–reperfusion injury, only a few studies investigated the effects of this oil on functional recovery. Logic would dictate that inhibition of myocardial arrhythmia would ultimately lead to increased functional recovery. However, to our knowledge, no evidence exists to substantiate whether fish oil supplementation may lead to myocardial protection from ischaemia–reperfusion injury.
Benefits of fish oil supplementation
Dietary fish oil supplementation may play a role in preventing heart disease, heart attacks and atherosclerosis, by reducing serum TAG and LDL-cholesterol(Reference Díaz, Gómez and Nova58, Reference Chiu, Wang and Chien88, Reference D'Allesandro, Chicco and Lombardo102). Dietary supplementation of the individual fatty acids, EPA and DHA, showed similar effects to fish oil supplementation. It has been suggested that DHA is the main active component of fish oil and that supplementation of DHA would have similar effects as in fish oil supplementation(Reference Mclennan and Abeywardena90). Evidence also suggests that fish oil can reduce diastolic blood pressure(Reference Theobald, Goodall and Sattar84, Reference Lee, O'Keefe and Lavie87) and it is known that a reduction in blood pressure is normally associated with better long-term cardiac health(Reference De Marco, De Simone and Roman104).
Fish oil may also reduce mortality after a cardiovascular incident, as it plays a role in reducing potentially fatal arrhythmias(Reference Charnock, Sundram and Abeywardena72, Reference McLennan, Howe and Abeywardena85, Reference McLennan89, Reference Mclennan and Abeywardena90, Reference Hlavackova, Neckar and Jezkova95). Little is, however, known about the effects of fish oil on infarct size and post-ischaemic functional recovery of the heart.
Palm oil
Refining of palm oil
Palm oil is produced by extraction of the oil from the mesocarp of the fruit of the Ealius genesis plant, commonly known as the oil palm. As for most oils, to be retailed as edible oil it also has to undergo certain refinery processes. Refining of palm oil requires bleaching and deodorisation which may destroy some of the important micronutrients contained in the oil(Reference Wilson, Nicolosi and Kotyla105).
A novel process has been developed by which palm oil can be refined in order to preserve more micronutrients. Carotenoids and vitamins which would normally be destroyed by the deodorisation and bleaching steps of the refining of palm oil are thus retained(Reference Nagendran, Unnithan and Choo106, Reference Sundram, Sambanthamurthi and Tan107) and these micronutrients, particularly the carotenoids. give red palm oil (RPO) its distinctive red colour.
Components of palm oil
Palm oil has a balanced fat composition of SFA and unsaturated fatty acids. Of these fats, 51 % are SFA, 39 % MUFA and 10 % PUFA (mostly linoleic acid)(Reference Wilson, Nicolosi and Kotyla105, Reference Ong and Goh108). Palmitic acid is the major SFA in palm oil, making up 44 % of the total fatty acids, with the other 7 % SFA being made up by myristic acid and stearic acid. Most of the MUFA in palm oil are oleic acid. The vitamin E content of palm oil is approximately 62 mg/g, of which 16 mg/g is made up by α-tocopherol, with the balance being made up mostly by tocotrienols (70 %)(Reference Wilson, Nicolosi and Kotyla105, Reference Sundram, Sambanthamurthi and Tan107, Reference Ooi109). Very little of the carotenoids present in crude palm oil survives the conventional refinery process. The carotene levels in refined, bleached and deodorised palm oil may be as low as 1·2 mg/g(Reference Wilson, Nicolosi and Kotyla105).
RPO has a similar fatty acid profile to refined palm oil, with 51 % SFA, 38 % MUFA and 11 % PUFA(Reference Nagendran, Unnithan and Choo106–Reference Ong and Goh108). In addition to the fatty acids contained in RPO there is also an abundance of micronutrients naturally occurring in this oil. The colour of the oil is obtained from its high concentrations of carotenoids. RPO contains at least 500 parts per million of carotenoids which include α-, β- and γ-carotene along with some lycopene and xanthophylls. Of these carotenoids, 80–90 % are α- or β-carotene, which occurs in a ratio of 2:1 in the favour of β-carotene (375 mg/g)(Reference Lehninger, Nelson and Cox110, Reference Ping111). RPO is also unique among oils due to its high vitamin E content. It contains 560–1000 parts per million of vitamin E, of which approximately 18–22 % is tocopherols and 78–82 % tocotrienols(Reference Schroeder, Becker and Skibsted27, Reference Nagendran, Unnithan and Choo106, Reference Sundram, Sambanthamurthi and Tan107). This makes RPO the oil with the highest content of tocotrienols of all vegetable oils. The most abundant tocotrienol in RPO is γ-tocotrienol, which is a potent antioxidant that reduces cholesterol production and platelet aggregation(Reference Steiner and Anatasi112–Reference Pearce, Parker and Deason116). Serbinova et al. (Reference Serbinova, Khavaja and Catudioc117) showed that tocotrienols are forty to sixty times more potent as an antioxidant than tocopherol in a study done on palm oil vitamin E in an isolated perfused rat heart model. RPO also contains squalene, phytosterols and co-enzyme Q10(Reference Nagendran, Unnithan and Choo106, Reference Sundram, Sambanthamurthi and Tan107, Reference Goh, Choo and Ong118). RPO has been the focus of many studies aimed at improving the vitamin A status of certain communities(Reference Lietz, Henry and Mulokozi119–Reference Radhika, Bhaskaram and Balakrishna121). The main reason for RPO being the focus of these studies is the high carotene content of the oil and the major finding was that RPO is effective in combating vitamin A deficiency by the provision of ample carotene in the diet. RPO may thus prevent complications associated with vitamin A deficiency.
Cardiovascular studies with palm oil
Effects of palm oil on serum lipids
Most of the studies done on palm oil focused on its effect on the serum lipid profile(Reference Theriault, Chao and Wang54, Reference Kritchevsky55, Reference Qureshi, Qureshi and Weight115, Reference Hornstra122–Reference Kritchevsky, Tepper and Kuksis127). Historically the perception of dietitians and clinicians was that palm oil would have a negative effect on the serum lipid profile, due to the relatively high levels of SFA in the oil. However, research has proven this perception to be wrong, and suggested possible mechanisms by which palm oil may in fact reduce serum cholesterol. First, palmitic acid does not have an impact negatively on the serum lipid profile(Reference Kritchevsky55, Reference Ong and Goh108, Reference Khosla and Sundram128). Second, the TAG conformation in palm oil has SFA on the sn-1 and sn-3 positions of the glycerol backbone, and unsaturated fatty acids on the sn-2 position in 75–87 % of these molecules. This TAG conformation leads to absorption of more unsaturated fats than saturated fats, as the fatty acid on the sn-2 position is absorbed preferentially to those in the sn-1 and sn-3 positions(Reference Theriault, Chao and Wang54, Reference Kritchevsky, Tepper and Kuksis127, Reference Renaud, Ruf and Petithory129). Furthermore, tocotrienols contained within palm oil have been shown to inhibit HMG-CoA reductase, a rate-limiting enzyme in cholesterol biosynthesis(Reference Theriault, Chao and Wang54, Reference Qureshi, Qureshi and Weight115, Reference Qureshi, Bradlow and Brace124, Reference Khor, Chieng and Ong130). RPO has a similar fatty acid composition to refined palm oil, and thus it also has a neutral effect on the serum lipid profile. It also has a similar tocotrienol content to refined palm oil. It may therefore be accepted that RPO has similar effects on the serum lipid profile to palm oil.
In a study done on rabbits, Hornstra(Reference Hornstra122) found that palm oil was significantly less atherogenic than fish oil, linseed oil and olive oil. These results were obtained after the diet of these rabbits was supplemented with the different oils for 18 months. Wilson et al. (Reference Wilson, Nicolosi and Kotyla105) investigated the effect of palm oil on cholesterol concentrations and aortic cholesterol accumulation in a hypercholesterolaemic hamster model. They found that supplementation of 10 % palm oil to a standard hamster diet for 10 weeks had reduced total cholesterol, non-HDL-cholesterol and TAG when compared with a coconut-supplemented group. In addition, they demonstrated that palm oil supplementation can be associated with significantly lower lipid hydroperoxide formation than coconut oil supplementation. The authors suggest that this may be due to different antioxidant pathways associated with the oils, or an intrinsic effect of dietary fats. In the same study, RPO also reduced total cholesterol and non-HDL-cholesterol when compared with a coconut oil-supplemented group. In addition, the RPO led to a significant increase in HDL-cholesterol in this study. Results showed that RPO had the highest non-esterified cholesterol:cholesteryl ester ratio, indicating that it was the least atherogenic of all the oil preparations used. Zhang et al. (Reference Zhang, Wang and Xue131) showed that RPO did not change the serum lipid profile of volunteers fed a RPO-supplemented diet (60 % of fat was RPO) for 42 d. They also found that the carotenoid and tocopherol levels of the volunteers were significantly increased after the diet period. Qureshi et al. (Reference Qureshi, Qureshi and Weight115) supplemented fifteen hypercholesterolaemic subjects with 800 mg tocotrienol-rich fraction (TRF; extracted from palm oil) for 4 weeks. There was a 20 % reduction in total cholesterol and a 27 % reduction in LDL-cholesterol in these subjects after the 4 weeks. In another study, the same authors showed that supplementation of a blend of tocotrienols for 4 weeks could reduce the total cholesterol levels of hypercholesterolaemic individuals by 10 %. In a study by Salinas et al. (Reference Salinas, Márquez and Sutil132) it was found that a 35 d supplementation of a deodorised and bleached form of palm oil could significantly reduce total cholesterol and increase HDL-cholesterol in rats with induced hyperlipidaemia by the addition of 5 % egg yolk powder to the diet. It was concluded that the high concentrations of micronutrients and MUFA in palm oil altered the serum lipid profile favourably in hyperlipidaemic rats. In a study by Girardet et al. (Reference Girardet, Jacotot and Mendy74), rats were fed a diet containing 12 % palm oil for 1 year and found that palm oil caused increased total serum cholesterol, but that it was accompanied with a lower aortic accumulation of cholesteryl esters when compared with SFO, rapeseed oil, soyabean oil and butter. In the light of previous studies the palm oil-associated increase in cholesterol in this study may be due to the longer feeding period.
Effects of palm oil on cardiac arrhythmia
Research has shown that palm oil may have some anti-arrhythmogenic properties. Charnock et al. (Reference Charnock, Sundram and Abeywardena72) demonstrated that palm oil had some efficacy in reducing arrhythmia during reperfusion. These authors supplemented rats with 12 % fat in the diet for 1 year, before inducing ischaemia and reperfusion in vivo. They also noted that the incidence of ventricular fibrillation decreased in the palm oil group when compared with a control group which was supplemented with sheep fat. Abeywardena & Charnock(Reference Abeywardena and Charnock133) found that that the anti-arrhythmogenic effect of palm oil was significantly lower than that of SFO. In this study, rat diets were supplemented for 9 months with 12 % of the oils before induction of ischaemia–reperfusion.
Palm oil in the ischaemia–reperfusion model
The tocotrienols present in palm oil have been shown to offer protection from myocardial ischaemia–reperfusion injury in the isolated perfused rat heart model(Reference Simopoulos70, Reference Serbinova, Khavaja and Catudioc117). In both studies, vitamin E components of palm oil were fractionated from the oil. Serbinova et al. (Reference Serbinova, Khavaja and Catudioc117) perfused isolated rat hearts with TRF. They found that TRF could increase post-ischaemic functional recovery and suggested that the antioxidant activity of the TRF was responsible for the protection. In the study by Das et al. (Reference Das, Lekli and Das71), individual isoforms of tocotrienols (α, β and δ) were fed to rats by oral administration. Three concentrations of tocotrienols (0·35, 1 or 3·5 %) were fed to the rats for 2 or 4 weeks. Das et al. (Reference Das, Lekli and Das71) demonstrated a decreased infarct size with tocotrienol supplementation when compared with a control group. Similar decreases in infarct size could be achieved with α-tocotrienol and γ-tocotrienol supplementation. The authors proposed that the antioxidant activity of tocotrienols may play a role in the protection offered against ischaemia–reperfusion injury, but emphasised the ability of tocotrienols to alter protein kinase signalling. In this study, decreased infarct size was accompanied by increased phosphorylation of protein kinase B (PKB)/Akt.
Palm oil supplementation may also reduce oxidative stress associated with ischaemia–reperfusion injury. Narang et al. (Reference Narang, Sood and Thomas134) supplemented 5 or 10 % palm oil to the diet of rats for 30 d. They found an increase in glutathione peroxidase, catalase and superoxide dismutase activities in the hearts of rats supplemented with palm oil. This was associated with lower thiobarbituric acid-reactive substance measurements. The authors concluded that palm oil augments the activities of endogenous antioxidant enzymes, and thus reduces oxidative stress.
To our knowledge, only a few studies have been conducted on dietary RPO supplementation related to cardiovascular health. Most studies on RPO dealt with vitamin A deficiencies. However, in recent studies the effect of RPO in the ischaemic–reperfusion model was investigated. The group of Van Rooyen et al. have published several papers on the protective effect of RPO against ischaemia–reperfusion injury(Reference Esterhuyse, Du Toit and Van Rooyen135–Reference Engelbrecht, Odendaal and Du Toit142).
Esterhuyse et al. (Reference Esterhuyse, Du Toit and Van Rooyen135) demonstrated that dietary RPO supplementation could offer protection against ischaemia–reperfusion injury in the isolated perfused heart. These authors fed rats 200 μl RPO per d for 5–6 weeks before perfusions were performed. Recent studies published on RPO have mostly used the ischaemic–reperfused isolated rat heart model(Reference Esterhuyse, Du Toit and Benade136, Reference Bester, Van Rooyen and Du Toit137). A follow-up study showed that dietary RPO supplementation could offer similar protection in cholesterol-fed rats where rats were placed on a diet that included 2 % synthetic cholesterol with or without 200 μl RPO(Reference Esterhuyse, Du Toit and Benade136). The cholesterol-supplemented group showed decreased functional recovery when compared with standard rat chow-fed controls. RPO supplementation could, however, significantly increase functional recovery in the hearts of cholesterol-supplemented animals. Esterhuyse et al. (Reference Esterhuyse, Du Toit and Benade136) suggested that protection was offered in cholesterol-fed rats via a different pathway when compared with rats fed a standard rat chow diet based on the finding that the NO–cyclic GMP signalling pathway was not affected by RPO supplementation in cholesterol-fed rats.
Bester et al. (Reference Bester, Van Rooyen and Du Toit137) showed that dietary RPO supplementation could offer protection against ischaemia–reperfusion injury, irrespective of the fat content of the diet. In this study the diets were designed to be isoenergetic to investigate whether increased energy in the RPO group may have played a role in previous studies. One diet contained high levels of SFA, while the other contained high levels of PUFA. RPO supplementation was able to offer protection against ischaemia–reperfusion injury in both diets when compared with controls. These authors suggested several mechanisms for RPO-mediated protection against ischaemia–reperfusion injury. Amongst the proposed mechanisms are the NO–cyclic GMP pathway, phosphorylation of mitogen-activated protein kinases and scavenging of deleterious ROS by RPO(Reference Esterhuyse, Du Toit and Benade136–Reference Kruger, Engelbrecht and Esterhuyse140). In the study by Engelbrecht et al. (Reference Engelbrecht, Esterhuyse and Du Toit138), the RPO supplementation caused increased phosphorylation of PKB/Akt and p38, and decreased phosphorylation of c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in rats fed a standard rat chow diet supplemented with 200 μl RPO for 5–6 weeks. These findings were accompanied by decreased caspase activation and poly ADP ribose polymerase (PARP) cleavage, as well as improved aortic output recovery, indicating a protective effect. The mitogen-activated protein kinase activation was markedly different when a similar diet was supplemented with 2 % cholesterol(Reference Kruger, Engelbrecht and Esterhuyse140). These authors supplemented cholesterol and RPO simultaneously for 5–6 weeks in order to determine whether cholesterol supplementation altered the effects of RPO on mitogen-activated protein kinase signalling. They found increased caspase activation and decreased PARP cleavage with RPO supplementation. This was accompanied by increased ERK phosphorylation together with decreased JNK and p38 phosphorylation in the RPO-supplemented group when compared with the cholesterol-fed controls. This indicates that RPO supplementation activated survival kinase pathways and inhibited apoptotic pathways. These changes led to a significantly greater aortic output recovery in the RPO-supplemented group than in the cholesterol-fed controls. Engelbrecht et al. (Reference Engelbrecht, Odendaal and Du Toit142) found in a similar model that dietary RPO supplementation increases PKB/Akt phosphorylation via a phosphatidylinositol 3-kinase (PI3K)-independent pathway. They perfused hearts in the presence of wortmannin, which is a known inhibitor of PI3K. Despite inhibition of PI3K in this study, RPO could still induce increased phosphorylation of PKB/Akt, and induce increased reperfusion functional recovery.
The above-mentioned studies showed that hearts of dietary RPO-supplemented rats have significantly increased cyclic GMP levels early in ischaemia(Reference Esterhuyse, Du Toit and Van Rooyen135–Reference Bester, Van Rooyen and Du Toit137, Reference Esterhuyse, Van Rooyen and Strijdom139). Hearts of dietary RPO-supplemented rats showed increased levels of NO within the myocytes. These findings suggest that dietary RPO supplementation leads to an increased NO conservation in the myocytes, and therefore stronger NO–cyclic GMP signalling. Both these signalling molecules are antagonists of cAMP and would thus offer protection against ischaemia–reperfusion injury by blocking intracellular Ca overload(Reference Du Toit, Meiring and Opie143). Khairallah et al. (Reference Khairallah, Khairallah and Gélinas144) suggested that increased levels of cyclic GMP may prevent intracellular TAG accumulation in cardiomyocytes and that this may offer further protection. Van Rooyen et al. (Reference Van Rooyen, Esterhuyse and Engelbrecht141) argued that not one, but a few mechanisms may be involved in the protective effect of RPO against ischaemia–reperfusion injury. Therefore, it is not conclusive that the pro-survival Akt pathway, the anti-apoptotic pathway or the NO–cyclic GMP pathway alone is responsible for this protection. The complexity of the composition of the oil, including 50 % unsaturated fatty acids and several highly potent antioxidants, may well be the strong point of this oil.
Benefits of palm oil supplementation
Palm oil may be able to offer protection against ischaemia–reperfusion injury, through the TRF contained in it(Reference Das, Lekli and Das71, Reference Serbinova, Khavaja and Catudioc117). Palm oil may also lead to the reduction of oxidative stress, and this could play a role in the reduction of ischaemia–reperfusion injury(Reference Narang, Sood and Thomas134).
It has also been suggested that palm oil may have some anti-arrhythmogenic effects, which may reduce sudden death after ischaemic incidents(Reference Charnock, Sundram and Abeywardena72).
Palm oil may also exert a neutral or positive effect on the serum lipid profile through the effects of its fatty acid composition and tocotrienols(Reference Theriault, Chao and Wang54, Reference Kritchevsky55, Reference Qureshi, Qureshi and Weight115, Reference Hornstra122–Reference Kritchevsky, Tepper and Kuksis127).
Few studies have been performed using RPO that focused on the baseline effects of this oil on cardiovascular health. RPO supplementation does, however, offer protection against myocardial ischaemia–reperfusion injury via several suggested mechanisms(Reference Esterhuyse, Du Toit and Benade136–Reference Van Rooyen, Esterhuyse and Engelbrecht141). The mechanisms suggested for the aforementioned protection include up-regulation of the NO–cyclic GMP signalling pathway and increased phosphorylation of PKB/Akt.
This oil has positive effects on the serum lipid profile and reduces oxidative stress(Reference Das, Lekli and Das71, Reference Wilson, Nicolosi and Kotyla105, Reference Zhang, Wang and Xue131, Reference Salinas, Márquez and Sutil132). Furthermore, as refined palm oil and RPO have comparable contents except for the higher micronutrient (mainly carotenoids) content of RPO, RPO may exhibit similar anti-arrhythmogenic effects, as is suggested of palm oil(Reference Charnock, Sundram and Abeywardena72).
RPO has been shown to be effective in reducing vitamin A deficiency and associated ocular disorders. This is due to the pro-vitamin A provided by RPO(Reference Lietz, Henry and Mulokozi119–Reference Radhika, Bhaskaram and Balakrishna121).
The refinery process of RPO, which retains more of the micronutrients, makes it unnecessary to add more antioxidants to the oil. Addition of antioxidants to palm oil does not offer similar protection as natural RPO(Reference Wilson, Nicolosi and Kotyla105).
RPO contains high concentrations of tocotrienols and carotenoids and research has shown that different carotenes may not only work synergistically with each other, but also with tocotrienols to achieve greater antioxidant effects(Reference Stahl and Sies26, Reference Schroeder, Becker and Skibsted27).
Discussion and conclusion
More research needs to be done on edible oil products. Many studies focus on only certain components of the oils that are normally employed during research. There also seems to be a trend that each oil is only used in studies to obtain specific types of endpoints which have previously been associated with that oil. These approaches frustrate any attempt to speculate what the effects of dietary supplementation of edible oils on cardiovascular health would be. In our opinion, the best way to determine the effects of dietary edible oil supplementation on the cardiovascular system would be to supplement the whole oil (unmodified) to the diet of research subjects and subsequently measuring a broad spectrum of endpoints, irrespective of the oil used.
From the studies that have been done, however, it is clear that the oils that have been reviewed in the present article all may form part of a healthy diet. Each oil seems to have some beneficial effects. It may be that some undesirable effects are still to be elucidated in these oils and there is still controversy about the beneficial effects of some of these oils. However, from the current literature, these oils all have more beneficial effects on cardiovascular health than detrimental effects, if supplemented to the diet in acceptable proportions.
Most studies performed with olive oil focused on the anti-oxidative effects of the polyphenols in this oil and many researchers used only extracts of the antioxidants in the olive oil to perform their studies. The inhibition of oxidative stress along with the reduction of serum cholesterol, which is associated with olive oil, would lead to a reduced risk of IHD. However, it is difficult to predict if this oil would have any protective effects against ischaemia–reperfusion injury. It is also unclear if this oil would have anti-arrhythmogenic effects. More research needs to be done using this oil as a dietary supplement, with the focus of elucidating its effects on ischaemia–reperfusion injury. This oil may, however, be advised for use to individuals with dyslipidaemia, high blood pressure or a family history of heart disease.
SFO has mostly been used as a control to compare other oils with. The only clear effects of this oil on cardiovascular health are reduction of serum cholesterol, and some mild anti-arrhythmogenic effects. This oil has also been shown to have a pro-oxidant effect if used for frying. Use of SFO is therefore less desirable than the other oils mentioned in the present review.
Dietary fish oil supplementation inhibits arrhythmia in healthy individuals. This finding could not be repeated with regularity in individuals with a history of heart disease. More research needs to be done in order to elucidate the reasons for the variations in this effect by fish oil. Little is known of other effects that fish oil may have on the cardiovascular system. The effects of dietary fish oil supplementation on the risk factors for IHD, and its effects on ischaemia–reperfusion injury, should be investigated. Fish oil is, however, the only oil that can with any certainty be recommended to individuals who are at risk of cardiac arrhythmia.
Palm oil has mostly been used in studies to determine its effects on the serum lipid profile. This oil has a neutral effect on the serum lipid profile and may even reduce atherosclerosis and prevent IHD. The few studies done with palm oil on arrhythmogenesis are non-conclusive. However, palm oil may have some minor anti-arrhythmogenic effects. Furthermore, the studies performed with TRF extracts from palm oil suggest that palm oil may offer protection against ischaemia–reperfusion injury. These effects, however, should be subjected to further study in a dietary supplementation model.
An advantage of doing research on the effects of RPO is that it contains the same components as refined palm oil, except for the presence of more micronutrients. We can therefore assume investigations performed using palm oil would also apply to RPO. Studies performed with RPO confirm this assumption, and additionally the presence of more micronutrients often led to enhanced effects due to their synergistic action. RPO was shown to have a neutral or slightly lowering effect on the serum lipid profile. Furthermore, our research has shown that RPO offers protection against ischaemia–reperfusion injury when supplemented to the diet. More research should be done with RPO to confirm its effects on risk factors for IHD and cardiac arrhythmia.
As palm oil has only mild effects on the serum lipid profile it is not known whether individuals who are at risk of CVD will benefit from its consumption. However, it is highly advised that these individuals do consume this oil, as it is one of the few oils that have been shown to effectively protect against myocardial ischaemia–reperfusion injury.
Acknowledgements
The present review received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.
D. B. and J. V. R. contributed mostly to the design and writing of the manuscript, while A. J. E. and E. J. T. were involved in reviewing the manuscript.
The authors confirm that there are no conflicts of interest.
