The study pertains to phenotypic characterization of some breeds of duck (common white duck and Indian runner duck), Muscovy ducks (black and white feathered and sepia feathered) besides two breeds of geese (white and brown feathered and white feathered) reared in the state of West Bengal (India). The study was conducted at five locations of the state and comprised 1395 ducks and 600 geese. The data were analysed statistically using mainly descriptive statistics, the differences in mean were analysed using Duncan's multiple range test. Both qualitative and quantitative traits were considered in the study. The qualitative traits were shank colour, bill colour, colour of the feathers, skin colour, egg shell colour, the presence or absence of beans, body carriage, bill type and colour of the eyes, while the quantitative traits included in the study were weight of the ducks and weight of the eggs. Some production traits such as numbers of eggs laid per year and dressing percentage of the drakes and ganders of each breed too have been studied. The ducks and geese are raised under semi-intensive management system and mostly depend on scavenging for their nutritional needs. This results in poor egg production and at times loss of stock because of diseases. The study regarding average egg production indicated that the Muscovy ducks and the geese have poor egg production, whereas the Indian runner followed by the common white duck are potentially the best. The ducks are important source of earnings for the small holder farmers and are raised both for meat and eggs, while the geese are reared mostly for ornamental purpose. However, the populations of the ducks are fast dwindling because of avian influenza in the region and because restocking is mostly being carried out with less adapted Khaki Campbell ducks. This happens mainly because the government farms that were responsible for providing the ducklings for restocking maintain Khaki Campbell ducks and do not have the parental stock of the native duck breeds, while restocking of the Muscovy ducks and geese are carried out by the rearers themselves. There are also no prevailing breeding programmes to conserve, select, maintain and distribute the native breeds of duck and geese. Hence, it is of earnest importance to conserve the avian genetic resources before the population dwindles further.

Village chickens have been kept for millennia under patronage of smallholder farmers. Our study was intended at dissecting the signature of artificial selection and ecological variation on morphological structures of Ethiopian village chickens. This report was based on visual traits of 798 chickens and a concise one-to-one interview of 399 farmers for their preferences on chicken morphology. Significant population-specific differences in morphological counts were commonly found for rare morphological variants. Most of them were frequently seen in Jarso chickens, while some of them unique to Jarso chickens. This might be explained by the effect of location-specific evolutionary forces and differences in their breeding histories. The high within population variation in the frequency of morphological counts was observed among these panmictic chicken populations largely evolved under uncontrolled mating. Single comb was not (less) preferred by majority of the farmers (93.8 percent); it was thus present at a low frequency (26.7 percent). Farmers have shown high preference for yellow shank (42.3 percent), which was then frequently observed (61.1 percent). The reported reasons for morphological likeness were visual appeal, market demand and cultural and religious values. The absence of significant variation in preferences for chicken morphology among communities between the two study sites was attributed to their multifunctional needs.

The American Shire horse breed is currently listed in “critical condition”, the most serious threat category assigned by the American Livestock Breeds Conservancy. Despite concern for the breed's future, however, a thorough genetic analysis of the breed has not been conducted to date. Pedigree data from 85 671 Shire horses born between 1806 and 2011 were used to determine current and historical genetic variability in the Shire horse since the first imported animals arrived from the United Kingdom to the United States. From this larger dataset, a reference population of 1 152 animals representing the most current generation was defined. Effective population size was 330.5, with 832 total founders and high pedigree completeness (98.2 percent) to five generations. Effective number of founders, ancestors and genomes were 104.5, 28.6 and 13.9, respectively. Average inbreeding coefficient was 2.4 percent from complete pedigree data and 1.3 percent when the most recent five generations were considered. Demographic analysis also revealed a population bottleneck in the 1950s. The ten most influential male and female ancestors contributed 48.5 percent and 11.6 percent, respectively, to the reference population, and only three foundation sires are responsible for 95.6 percent of all sire lines in the current generation. Although current assessment of genetic variability indicates a relatively healthy population, the small number of breeding animals, low birth rates and steady rise in inbreeding coefficients mark the American Shire as an at-risk breed for which conservation strategies should be considered.

Information on morphological characteristics is a prerequisite to sustainable breed improvement, utilization and conservation. The present study was initiated to describe the physical and morphological characteristics of indigenous sheep reared in Sidama-Gedeo, Kembata Tembaro-Hadiya, Gurage-Silte, Gamogofa and Wolaita zones of Southern Regional State of Ethiopia. Data on the assessment of qualitative characters and linear body measurements were obtained from a total of 3966 sheep that were drawn from 928 households. The results indicated that the most common tail form in the Sidama-Gedeo, Kembata Tembaro-Hadiya, Gurage-Silte and Wolaita sheep was of long fat type ending with a straight tip, whereas that of Gamogofa sheep was long thin tail ending with a curve tip. Hair coat colour pattern was mainly plain followed by patchy. The common hair of sheep in all studied zones was short and smooth. Sheep from Sidama-Gedeo, Kembata Tembaro-Hadiya and Gurage-Silte were devoid of wattle and ruff. Rams of Gurage-Silte and Wolaita had higher (P < 0.05) body weight values than those of other locations. Ewes of Sidama-Gedeo and Wolaita had relatively high (P < 0.05) body weight, whereas those of Gurage-Silte, Gamogofa and Wolaita had significantly high chest girth values. Gurage-Silte and Gamogofa rams had large (P < 0.05) chest girth values. The height at withers was high (P < 0.05) for both sexes in Sidama-Gedeo, Kembata Tembaro-Hadiya and Wolaita sheep. The highest body length was obtained from Wolaita rams, followed by Gamogofa and Kembata Tembaro-Hadiya rams, whereas the lowest body length was obtained from Gurage-Silte rams. Ewes of Sidama-Gedeo had higher (P < 0.05) body length values than those of other zones. The dendrogram indicated a close association among the sheep of Kembata Tembaro-Hadiya, Gurage-Silte and Sidama-Gedeo. However, sheep in Wolaita and Gamogofa were more distantly associated with those of the three zones. It can be concluded that the sheep in the study zones may possess unique adaptive features useful in designing sustainable sheep improvement strategies. However, a molecular-based genetic assessment of the population structure found in the study region might be given due consideration.

A total of 700 adult female goats from Sétif area (Algeria), were investigated for eight qualitative morphological traits and 17 body measurements. Sampling included three environmental areas: Northern, Central and Southern area. Coefficients of variation ranged from 10.9 percent to 34.7 percent, showing high heterogeneity. Canonical analyses showed that differences in body measurements between the three area-populations were large and significant in all traits (P < 0.001). The shortest Mahalanobis distance was found between North-Center populations (2.8), while that between Center (4.0) and (8.6) between North-South populations were the largest. Discriminant analysis showed that most goat-populations were classified into their environmental-area, North (73.0 percent), Center (66.8 percent) and South (79.3 percent) with a few misclassified individuals. Correspondence analysis indicated no large differences between the goat populations; they were more homogeneous for qualitative traits. This information will constitute the basis for further characterization and develop conservation strategies for Algerian goat populations

Ghumusar is an inadequately studied goat population of western region of Ganjam district of Orissa state. Sporadic information is available on its morphological traits but no information is found on its genetic variability. Therefore, an attempt was made to measure the genetic diversity in Ghumusar goat population using 25 microsatellite markers. Genomic DNA isolated from blood samples drawn at random from 50 individuals were utilized for this study. The average number of observed allele was 9.80 and the effective average number of allele was 4.28. The polymorphic information contents ranged from 0.53 to 0.91. The average observed and expected heterozygosity was 0.73 and 0.71, respectively. The values of Nei's gene diversity in Ghumusar goat population ranged from 0.11 to 0.87 with a mean of 0.69. The overall Fis value was observed to be 0.002, which is not significantly different from zero; hence indicating no global deficit of heterozygotes. Under sign test, expected number of loci with heterozygosity excess (Hee) was 14.88, 14.79 and 14.74 for the infinite allele model (IAM), stepwise mutation model (SMM) and two-phased model (TPM) of mutation, respectively. The observed number of loci with heterozygotic excess (He) was 14, 8 and 2 under these three models, respectively. Under all the three models, He was less than Hee and this deviation was significant under SMM and TPM models. There was no serious genetic reduction in effective population size as indicated by L-shaped curve in Ghumusar goat population.

This study aims to characterize Azikheli, an undocumented buffalo breed, in its home tract (Khwazakhela, Swat, Pakistan) under traditional farming conditions. For this purpose, 108 buffalo cows and 27 bulls were randomly selected. Mean, standard error, Student's t test and Chi-square test were used for various comparisons. The results show that the majority of animals have a brown coat colour. Cows have significantly higher heart girths, longer horns, longer necks and wider faces at the level of the eyes than bulls, whereas bulls have significantly longer bodies, longer ears, thicker horns, thicker necks and larger hooves than cows. Horns are flat laterally, directed backwards and then slightly upwards without twisting, leading to a sickle to semi-sickle appearance. Owing to its small body size and brown coat colour, the breed is well adapted to mountain slope grazing and thrives well away from swamps. Its adaptation to mountainous ecosystems warrants its in situ conservation.

An exploratory survey of local cattle populations of Gamo Goffa Zone in south-western Ethiopia was conducted between April 2011 and May 2012 to identify and phenotypically characterize cattle populations. Ten focus group discussions with key informants as well as phenotypic data from sample mature animals were used to generate data, including quantitative trait data from 560 animals and qualitative trait data from 867 animals. Findings from focus group discussions revealed that, even if local cattle are found widely distributed throughout the study area, they are not known by any common name or breed type. Results from analysis of variance (ANOVA) on continuous variables showed significant (P < 0.0001) differences between sites. Tukey's multiple mean comparison test showed that each quantitative traits were significant (P < 0.0001) for sites. Chi-square test was also significant (P < 0.0001) for most of the categorical variables per sites. Based on a discriminant analysis, sample populations were classified into their respective sites with overall hitting rate was 63.15 percent for females and 74.89 percent for males. Canonical discriminant (CANDISC) analysis showed quantitative traits and Mahalanobis' distances between sites were significant (P < 0.0001). The stepwise discriminant (STEPDISC) analysis for both populations showed that most variables had significant (P < 0.0001) power in explaining phenotypic variation. These information from focus group discussions and phenotypic variation analyses led to identification of two cattle populations that deemed to be distinct breed types (Gamo highland and lowland). Thus, indigenous cattle population of the study area was not homogenous on their phenotypic features, and further genetic characterization should be done to confirm their genetic distinctiveness.

Achai is an as yet undocumented but well-adapted cattle breed of the Hindu Kush Mountains of northern Pakistan, which is reared under sedentary farming system (SFS) and transhumant farming system (TFS). This paper compares the morphological (physical and morphometric) characteristics of this cattle breed under both farming systems to know the effect of these styles of management. Data were collected from 108 adult females and 108 males in SFS and 108 females and 36 males in TFS on physical characteristics (colour of the coat, horns, eyelashes, muzzle, hoof, switch) and morphometric measurements – i.e. heart girth, body length, height at withers, height at hipbone, face length, horn length, horn circumference, ear (length and width), neck length, dewlap length, chine length, loin length, rump (length and width), length below knee, hoof circumference, tail and switch length. Results showed that farming systems significantly affect most of the morphological characteristics of both sexes, particularly the morphometric measurements with tall and leggy conformation for TFS cows and bulls. This indicates an adaptation to long distance transhumance and mountain terrain grazing. It can be concluded that the Achai has the potential to adapt to the requirements of specific farming systems. There is hence a need for the conservation of its adaptive traits in both farming systems.

Mastitis is a frequent disease in modern dairy cows, but ancient cattle breeds seem to be naturally more resistant to it. Primary bovine mammary epithelial cells from the ancient Highland and White Park (n = 5) cattle and the modern dairy breeds Brown Swiss and Red Holstein (n = 6) were non-invasively isolated from milk, cultured, and stimulated with the heat-inactivated mastitis pathogens Escherichia coli and Staphylococcus aureus to compare the innate immune response in vitro. With reverse transcription quantitative polymerase chain reaction (RT-qPCR), the breeds differed in the basal expression of 16 genes. Notably CASP8, CXCL8, Toll-like receptors 2 and 4 (TLR2 and TLR4) expression were higher in the ancient breeds (P < 0.05). In the modern breeds, more genes were regulated after stimulation. Breed differences (P < 0.05) were detected in C3, CASP8, CCL2, CD14, LY96 and transforming growth factor β1 (TGFβ1) regulation. Principal component analysis separated the ancient from the modern breeds in their basal expression, but not after stimulation. ELISA of lactoferrin and serum amyloid A protein revealed breed differences in control and S. aureus treated levels. The immune reaction of ancient breeds seemed less intensive because of a higher basal expression, which has been shown before to be beneficial for the animal. For the first time, the innate immune response of these ancient breeds was studied. Previous evidence of breed and animal variation in innate immunity was confirmed.

The Vechur Cattle of South Kerala (India), once popular and known for its comparatively high milk yield compared with the other local varieties, had become almost extinct by the 1980s. This was due to the massive cross-breeding with exotic bulls implemented by the Government in order to enhance milk production. But the launching of a search for the last available specimens of the variety and the initiation of a conservation effort in 1989 in the Kerala Agricultural University by the author and a team of her students resulted in saving this valuable breed. With 10 years time the distribution of the progeny of the foundation stock to the farmers could be started. The conservation work was taken forward with strong determination in spite of unexpected events. The Vechur cattle was subsequently recognized as a cattle breed of India. The cow yields 2.5 litres of milk daily during the lactation period of about 8 months. It has emerged as a sustainable cow to farmers. The adaptability of the animal to the hot humid tropical countries has special significance as an insurance for the future. It also has the potential to be a small good beef breed. The value of the milk including the small fat globule size, presence of A2 beta-lactalbumin protein and higher arginine content has been proven scientifically. The total population size in Kerala is estimated to be 2000 animals.

This paper reviews experiences with cross-breeding for milk production in the tropics. Data were compiled from 23 different studies evaluating the performance of different grades of cross-bred animals as well as local breeds. Relative performance of indigenous breeds compared with different grades of cross-breeds was calculated for three climatic zones. Traits considered were milk yield per lactation, age at first calving, services per conception, lifetime milk yield and total number of lactations completed. At 50 percent Bos taurus blood, lactation milk yields were 2.6, 2.4 and 2.2 times higher than those of local cattle in the highland, tropical wet and dry, and semi-arid climatic zones, respectively; lactation lengths increased by 1.2, 1.2 and 1.9 months in the above-mentioned climatic zones, respectively; there was a reduction in calving interval by 0.8 times and in age at first calving by 0.9 times. Similarly, cross-breds with 50 percent B. taurus genes had 1.8 times higher lifetime milk yields and a 1.2 times higher number of total lactations. Although cross-breeding faces a number of challenges such as better infrastructure, higher demand for health care, there are many advantages of using it. These are higher production per animal, higher income for the families and provision of high-value food. It is therefore likely to continue to be an important livestock improvement tool in the tropics in the future, where farmers can provide sufficient management for maintaining animals with higher input requirements and access to the milk market can be secured.

Pedigree data from 11 267 animals born between 1960 and 2004 were used to analyse population structure and genetic variability of the Holstein–Friesian population in Kenya. Parameters estimated were the pedigree completeness index, average inbreeding coefficient, number of founders, effective number of founders and ancestors, and genetically important herds. The hierarchy of registered herds and concentration of origin of individuals was also assessed. Pedigree completeness of the reference population was 67.1 percent. Average inbreeding level for the entire population was 0.09 and 1.7 percent among individuals with three complete generations, and 9.2 percent among inbred individuals. Inbreeding level increased with generation from 0.8 to 2.5 percent in the most recent generation among individuals with three complete generations. Effective number of founders and ancestors were 156 and 108, respectively. The ten ancestors with the largest marginal genetic contribution accounted for 19.52 percent of the total variation. The effective number of genetically important herds that contributed breeding males to the population was 5.2. Higher levels of inbreeding were detected among individuals with at least three complete generations. Few herds contributed breeding males, causing structural weakness to the breeding programme. Recruitment of herds into the breeding tier is needed to strengthen the breeding structure and pedigree recording enhanced to enable long-term management of genetic variability.

The Curraleiro, also called Pé-Duro, is the only naturalized bovine breed of Northeastern Brazil. The breed originated via admixture of different Iberian breeds that were introduced during the sixteenth century and the subsequent natural adaptations to the semi-arid environment. The introduction of zebu bovine breeds during the twentieth century led to the replacement of the Curraleiro, which is now on the brink of extinction. With the aim of supporting the establishment of a conservation programme, the present study focused on assessing the management of the existing Curraleiro populations. Data on 2 366 head of cattle were obtained by interviewing 22 owners of Curraleiro herds. The number of animals in the herds varied from 4 to 384. The average herd size was 131.4 (standard deviation = 120.3). All animals were bred under extensive conditions, without any supplementary feed, and in 50 percent of all cases without any specialized technical assistance. Data indicated that breeders maintain the cattle not for economic reasons, but because of tradition. The Curraleiro was characterized by the breeders as being resistant to water stress and parasites. The animals can be bred without feed supplements on all kinds of native-grass pastures. These characteristics, in addition to its low production costs, could make the Curraleiro attractive, especially for low-income families.

Turkey's 21.8 million head of sheep comprise a rich array of fat-tailed and thin-tailed native breeds and crosses of these with animals of exotic origin. The structure of agriculture is such that most farms are predominantly subsistence oriented, of small size, keep few animals and provide only minimal inputs. Native breeds have evolved to meet these conditions and are generally well adapted to the natural environment, inadequate and unbalanced nutrition and disease stress. In the last 80 years, there have been attempts to modify the natural gene pool and many distinct breed types are in danger of or have already suffered extinction. This paper describes some of the major sheep genetic resources and their conservation status. The government is now aware of the danger of impoverishment or loss of this important aspect of biodiversity and has established programmes for conservation and preservation of several native breeds. Government, research institutions and producers should work together to ensure that the local gene pool is preserved and can thus continue to contribute to biodiversity and sustainable livestock production.