Weed control is one of the principal problems of turf grass management. With the discovery that 2,4–D would selectively kill many broad-leaved weeds their control in turf has been minimized. As has been indicated by Grigsby (9), the widespread usage of 2,4–D may be an important factor in increasing the importance of crabgrass (Digitaria spp.) as a turf weed.

A hypothesis that low temperature acts directly on physiological development of coast fiddleneck (Amsinckia intermedia Fisch. and Mey.) by restricting thiamin content, and limiting growth, was investigated. Restoration of normal growth by application of thiamin, made the plant more susceptible to 2,4-dichlorophenoxyacetic acid (2,4-D) at low temperatures.

Selective postemergence spraying of cotton in the western irrigated areas for control of weeds has not been widely practiced because of the lack of adequate herbicides and little knowledge of the effect of varying rates of the more promising herbicides on the growth, yield and fiber quality of cotton.

Field research to determine the influence of weed control systems in winter barley on subsequently no-till-planted grain sorghum was conducted from 1985 to 1987 in northwest Georgia. Herbicide treatments applied to fully tillered winter barley included 2,4-D, dicamba, metribuzin, oryzalin, oryzalin plus metribuzin, fluorochloridone, or thiameturon. None of these herbicides affected barley grain yields compared to nontreated plots. Treatments containing oryzalin reduced johnsongrass fresh weights in the subsequently planted grain sorghum. Oryzalin plus metribuzin, in 1 of 2 yr, resulted in higher seed yields of subsequently planted grain sorghum compared to the other treatments except for oryzalin used alone. Treatments containing paraquat plus propazine plus metolachlor applied to grain sorghum provided good weed control when averaged across barley treatments and resulted in the highest grain sorghum yield. No grain sorghum injury from herbicides used in the previous winter barley crop was observed.

About 25 ppm of 4-amino-3,5,6-trichloropicolinic acid (picloram) usually were detected on grass, primarily buffalograss (Buchloe dactyloides (Nutt.) Engelm.) and blue grama (Bouteloua gracilis Willd. ex HBK Lag ex Griffiths), immediately after application of 0.28 kg/ha picloram + 0.28 kg/ha (2,4,5-trichlorophenoxy)acetic acid (2,4,5-T) in northwest Texas. Less than 1 ppm of picloram usually was detected in grass tissue 30 to 60 days after treatment. At one location, detectable picloram increased in grass tissue from 32 to 60 days after application. Increases of picloram in aerial grass tissue were attributed to root uptake during a flush of vegetative growth. Picloram dissipation from grasses was not affected by irrigation to runoff at 10, 20, or 30 days after application. Detectable picloram was reduced by 93% in herbaceous, broadleaf species by 30 days after application. Treated sand shinnery oak (Quercus havardii Rydb.) leaves at the soil surface caused a slight increase of picloram in surface litter.

Five varieties of 3-month-old citrus rootstocks were grown for 1 month in nutrient solution containing 0, 10-3, 10-4, or 10-5 M of norflurazon [4-chloro-5-(methylamino)-2-(3-(trifluoromethyl)phenyl)-3 (2H)-pyridazinone] to assess the effect of this herbicide on growth and chlorophyll levels. The uptake, translocation, and metabolism of root-applied 14C-norflurazon was also examined in these rootstocks. Norflurazon treatment reduced chlorophyll level and lowered overall growth in sour orange (Citrus aurantium), Swingle (Poncirus trifoliata × C. paradisi), Milam (C. jambhiri), and Cleopatra mandarin (C. reshni) rootstocks. Herbicide treatments had little or no effect on chlorophyll levels or growth in Carrizo (C. sinensis × P. trifoliata). In Cleopatra, unlike in other rootstocks, leaf chlorosis was evident only along the midrib and major veins. Herbicide treatment reduced leaf flush (leaf production) in Swingle but not in other rootstocks. By the end of a 4-week treatment period, total absorption of 14C-norflurazon was greater in Swingle than in the other rootstocks. 14C-norflurazon detected in the roots (excluding taproot) of Swingle was greater than in the other rootstocks. The amount of 14C-norflurazon translocation to the foliage was limited in all rootstocks. Stem and taproot also contained only a small amount of 14C-norflurazon. Leaf extracts of all five rootstocks contained no parent compound, but up to 90% of recovered 14C was identified as 14C-norflurazon in root extracts. Differences in the uptake, translocation, and metabolism of norflurazon among citrus rootstocks were small and did not appear to explain their differential sensitivity to this herbicide.

Field research was conducted for 2 yr to determine the effects of reduced rates of bromoxynil on growth and yield of non–bromoxynil-resistant cotton. Rates of 4.5, 9, 17, 35, 70, and 140 g ha−1, representing 0.008, 0.016, 0.031, 0.063, 0.125, and 0.25 fractions of the maximum labeled use rate per application (560 g ha−1), were applied to cotton at the two-, five-, or nine-node growth stage. Visual injury was reduced because application timing was delayed from two- to five-node stage in all experiments and from five- to nine-node stage in two of three experiments. Although negatively affected at all application timings, plant height reduction response decreased with increasing cotton maturity. Plant dry weight was most negatively affected after application at the two-node stage. Bromoxynil application, based on the node above white flower number, did not result in maturity delays but did promote earlier maturity when applied at 140 g ha−1 to two- and five-node stage cotton in one of the three experiments. Final plant population was reduced only at the two- and five-node timings, with response more pronounced at the initial timing. Seedcotton yield after bromoxynil application at the highest rate to two-leaf cotton was reduced 34% compared with other rates and the nontreated control. Bromoxynil applied to five- or nine-node cotton did not significantly reduce yield.

Emergence of sugarbeets (Beta vulgaris L. ‘MonoHy D2’) from soil was reduced by germinating kochia [Kochia scoparia (L.) Schrad. ♯ KCHSC] seed at densities greater than 1 seed/cm2 when the fungus Rhizopus sp. was present. Water-soluble exudates from germinated kochia seed did not influence Rhizopus growth in vitro or sugarbeet germination. Cell-free culture extracts of Rhizopus reduced sugarbeet germination and radicle elongation. There appeared to be an interaction between kochia and Rhizopus, but the responsible compounds were not identified.

Field experiments were conducted to evaluate placement techniques for preemergence applications of pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine] in grain sorghum [Sorghum bicolor (L.) Moench.]. The first technique consisted of row shields mounted behind the planter units. Shields maintained an untreated strip over the crop drill and allowed successful crop establishment with pendimethalin at 1.1 kg ai/ha, despite a simulated, intense rainfall of 3.8 cm within 24 h after planting. A second technique, which consisted of a special nozzle arrangement, was evaluated in no-till grain sorghum. The nozzle arrangement allowed a broadcast herbicide application but maintained an untreated strip over the crop drill. No stand reductions occurred using this technique at pendimethalin rates of 1.1 and 2.2 kg/ha. In a growth chamber experiment, preemergence applications of pendimethalin severely injured grain sorghum when the soil was wet at the time of emergence, but injury was reduced under hot, dry conditions.

It is indeed a real pleasure for me to have the opportunity to participate in this third meeting of the Weeds Journal of Weed Society of America. The years I spent in the Weed Investigations Section were among the most rewarding of my professional life.

Weed and cucumber (Cucumis sativus L. ‘Poinsett’) growth was studied where herbicides were soil-applied or applied to emerged weeds in the established crop. Cucumber yields were decreased at least 36% in 2 of 5 yr when weeds interfered for 4 weeks after emergence. Sequential applications of bensulide [O,O-diisopropyl phosphorodithioate S-ester with N-(2-mercaptoethyl)benzenesulfonamide] applied preplant and incorporated (PPI) + trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) applied postemergence and incorporated (POI) were outstanding and controlled Japanese millet (Echinochloa crus-galli Link. var. frumentacea), Texas panicum (Panicum texanum Buckl.), Palmer amaranth (Amaranthus palmeri S. Wats.), and common purslane (Portulaca oleracea L.) without affecting the growth or yield of cucumber. Compared with bensulide, the selectivity of soil-incorporated butralin [4-(1,1-dimethylethyl)-N-(1-methylpropyl)-2,6-dinitrobenzenamine] with cucumber was limited. Laboratory bioassays showed that under warm field soil conditions, PPI applications of 1.7 kg/ha of butralin persisted more than 2 months, but were dissipated after 4 months. Ethalfluralin [N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl)benzenamine] reduced cucumber yields only when it was soil-incorporated to the crop-seed depth at planting. Applications to soil of bensulide + DCPA (dimethyl tetrachloroterephthalate) stunted cucumber plants, reduced leaf area, increased leaf thickness, and caused higher near-infrared (0.75 to 1.34 μm) leaf reflectance.

n-Pentane was used to extract 3-(m-trifluoromethylphenyl)-1,1-dimethylurea (fluometuron) from soil-water samples. The microgram quantity of fluometuron in the n-pentane was measured at a wavelength of 242 mμ with an ultraviolet spectrophotometer and this measurement related to the fluometuron concentration in the soil-water sample. No measurable interference was noted in the n-pentane after the extraction of fluometuron from the soil-water samples. The procedure was developed for extracting and quantitatively determining fluometuron in soil-water samples.

A method for isolating roots of a growing plant was devised. The technique allows treatment of an isolated portion of the root system without disturbance or injury to the untreated root mass. A specially designed planter-box was used to induce natural separation of the root system in 3 to 4 weeks. Weeping willow (Salix babylonica L.) seedlings were used to test the performance of dichlobenil (2,6-dichlorobenzonitrile), paraquat (1,1′-dimethyl-4,4′-bipyridinium ion), sodium hydroxide (NaOH), and copper sulfate (CuSO4 • 5H2O) in reducing the viability of treated roots. A tetrazolium chloride assay was used to determine the difference in viability of the treated roots. Copper sulfate and paraquat-treated plants produced symptoms of systemic injury.

A new machine consisting of a detachable hopper of containerized granules with a preselected circular orifice to regulate flow of granules, and a revolving hollow-cone spreader turned by an electric motor, was tested. Flow of herbicide granules at typical use rates was linearly correlated with orifice diameter. Coefficients of determination for flow rates of alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide], trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl)benzenamine], and norflurazon [4-chloro-5-(methylamino)-2-(3-(trifluoromethyl)phenyl)-3 (2H)-pyridazinone] were 0.96 or greater. Based on patterns of distribution of commercial trifluralin granules parallel and perpendicular to a linear path, the machine could be used to apply herbicide granules in row crops. Control of johnsongrass [Sorghum halepense (L.) Pers. # SORHA] with alachlor plus oryzalin [4-(dipropylamino)-3,5-dinitrobenzenesulfonamide] applied preemergence (pre) as sprays and granules did not differ significantly in soybean [Glycine max (L.) Merr. ‘Forrest′]. Trifluralin granules applied preplant incorporated (ppi) at 1.1 kg ai/ha controlled 80 to 95% of the johnsongrass and doubled soybean yield compared to the cultivated control plot. Norflurazon plus alachlor pre at 1.7 to 2.2 plus 2.2 to 3.0 kg ai/ha controlled annual grasses and prickly sida (Sida spinosa L. # SIDSP) equally with sprays and granules.

The phytotoxicity of postemergence propanil (3′,4′-dichloropropionanilide) to hard red spring (HRS) wheat (Triticum aestivum L.) was determined in the field and controlled environmental chamber. HRS wheat tolerance to propanil was influenced by cultivar, crop growth stage at time of application, and posttreatment temperature. Injury to wheat from propanil was greater at the five- than two-leaf stage of application and at 30- than at 10- or 20-C posttreatment temperatures. ‘Butte’ wheat was the most susceptible HRS wheat cultivar to propanil, with yield reductions ranging from 15 to 40% depending upon year and crop growth stage at application.

Timely results from whole-plant, herbicide-resistant weed screenings are crucial to heighten grower awareness. However, the high degree of physiological dormancy of giant ragweed (Ambrosia trifida L.) seed exacerbates this process. The most effective methods for alleviating dormancy, to date, are either labor-intensive (embryo excision) or require several weeks (soil stratification). This research describes a conditioning process involving clipping and aeration of seed in water that is highly effective at alleviating dormancy and requires less skill and time compared with previous techniques. Ambrosia trifida seeds were collected over 2 yr at two different collection timings (September 25, “early”; October 25, “late”), subjected to various treatments intended to release dormancy, and evaluated for emergence over 18 d in the greenhouse. The use of germination-promoting chemicals (ethephon, gibberellic acid, and thiourea) generally provided no increase in emergence compared with water and occasionally produced seedlings with abnormal growth unsuitable for further experimentation. Conditioning yielded between 30% and 33% emergence for both early and late collections of seeds with no afterripening period compared with 0% emergence for seeds imbibed in water. Following an 8-wk period of dry storage at 4 C, conditioning yielded nearly 80% emergence for both collection timings, while emergence of seeds imbibed in water was 10% and 27% for early and late collections, respectively. Soil stratification in moist soil for 8 wk at 4 C was the second most effective treatment, yielding 46% to 49% emergence across both collections. Parameters of the Weibull function further indicated the conditioning treatment had the fastest rate of emergence and shortest lag phase between planting and first emergence. Methods to germinate A. trifida without an afterripening period have previously been unsuccessful. Therefore, the seed-conditioning method outlined in this work will be useful in expediting the confirmation of herbicide-resistant A. trifida incidences.

Sweet corn (Zea mays L.) tolerance to dicamba and several other herbicides is due to cytochrome P450 (CYP)-mediated metabolism and is conferred by a single gene (Nsf1). Tolerance varies by CYP genotypic class, with hybrids homozygous for functional CYP (Nsf1Nsf1) being the most tolerant and hybrids homozygous for mutant CYP alleles (nsf1nsf1) being the least tolerant. The herbicide safener cyprosulfamide (CSA) increases tolerance to dicamba by stimulating the expression of several CYPs. However, the extent to which CSA improves the tolerance of different sweet corn CYP genotypic classes to dicamba is poorly understood. Additionally, the effect of growth stage on sweet corn sensitivity to dicamba is inadequately described. The objective of this work was to quantify the significance of application timing, formulation, and CYP genotypic class on sweet corn response to dicamba. Hybrids representing each of the three CYP genotypes (Nsf1Nsf1, Nsf1nsf1, nsf1nsf1), were treated with dicamba or dicamba + CSA at one of three growth stages: V3, V6, or V9. Across all timings, the nsf1nsf1 hybrid was the least tolerant to dicamba, displaying 16% higher crop injury levels 2 wk after treatment and 2,130 kg ha−1 lower ear mass yields compared with the Nsf1Nsf1 hybrid. The V9 growth stage was the most susceptible time for dicamba injury regardless of genotypic class, with 1.89 and 1,750 kg ha−1 lower ear mass yields compared with the V3 and V6 application timings, respectively. The addition of CSA to dicamba V9 applications reduced the injury from dicamba for all three genotypic classes; however, it did not eliminate the injury. The use of Nsf1Nsf1 or Nsf1nsf1 sweet corn hybrids along with herbicide safeners will reduce the frequency and severity of injury from dicamba and other CYP-metabolized herbicides.