The role of calcium in mediating resistance to several auxinic herbicides (i.e., 2,4-dichlorophenoxyacetic acid, [4-chloro-2-methylphenoxy] acetic acid, (±)-2-(4-chloro-2-methylphenoxy) propanoic acid [mecoprop], 3,6-dichloro-2-methoxy-benzoic acid [dicamba], or 4-amino-3, 5,6-trichloro-2-pyridinecarboxylic acid [picloram]) was investigated by modulating calcium dynamics of a susceptible (S) and resistant (R) biotype of wild mustard. The inhibitory effects of the auxinic herbicides on root length of the S seedlings were significantly reduced upon pretreatment with calcium in the presence of the calcium ionophore A23187. Conversely, the addition of verapamil, a calcium channel blocker, to the R seedlings increased their sensitivity to the auxinic herbicides. Valinomycin, a potassium channel ionophore, did not ameliorate the effect of the auxinic herbicides on both biotypes of wild mustard, thus indicating that the observed effects were specific for calcium. These results demonstrate that calcium plays a crucial role in the resistance of wild mustard to auxinic herbicides at the level of intact seedlings, thereby supporting our previous results using intact protoplasts.

Growth chamber experiments were conducted to evaluate the effects of relative humidity and temperature on the efficacy, absorption, and translocation of glufosinate at 205, 410, and 820 g ha−1 in Palmer amaranth, redroot pigweed, and common waterhemp. Low relative humidity decreased control of all three species by glufosinate. However, control increased as application rate increased at low relative humidity. Amaranth species grown under 21/16, 26/21, and 31/26 C day/night temperature regimes responded differently to glufosinate. At 26/21 C, glufosinate at 820 g ha−1 controlled redroot pigweed less effectively than it controlled Palmer amaranth and common waterhemp, whereas at 410 g ha−1, glufosinate controlled common waterhemp more effectively than it controlled the other two species. Neither temperature nor relative humidity altered the absorption of 14C-glufosinate in any of the three species. Most of the absorbed glufosinate remained in the treated leaves at all three temperature regimes and two relative humidity levels. However, glufosinate translocation was greater in plants grown at 90% than in those grown at 35% relative humidity, and this phenomenon coincided with greater control of the amaranth species at the high humidity level. The study showed that relative humidity had a greater effect than temperature on glufosinate toxicity to Palmer amaranth, redroot pigweed, and common waterhemp.

This study was conducted to ascertain movement potential of imazethapyr resistance and to measure the relative growth and productivity of imazethapyr-resistant (IR) and imazethapyr-susceptible (IS) biotypes of common sunflower under noncompetitive and competitive conditions. Susceptible biotypes of common sunflower were planted in the field in concentric circles at distances of 5.5, 8.0, 15.0, and 30.0 m around a center of densely planted IR biotypes in four locations in northeast Kansas in 1998 and 1999. Pollen movement was analyzed by sampling the IS progeny for the presence of imazethapyr resistance. The distance in which resistance is first detected from the IR pollen source, first unnatural resistant distance (FURD), ranged from 12.1 to 15.5 m. Wind direction was highly correlated with FURD; the north sections had larger FURD. Greenhouse studies were conducted to study growth of IR and IS biotypes under noncompetitive and competitive conditions. Under noncompetitive conditions, leaf area and dry weight were slightly greater for the IR than the IS biotype at early growth stages, but photosynthesis and height were similar. Under competitive conditions, photosynthesis, leaf area, height, and dry weight of IR and IS biotypes were similar. As a result, IR–IR and IS–IS intracompetition equaled IR–IS intercompetition. Gene flow from IR to IS biotypes occurred with movement up to 15.5 m. The lack of differences between growth of the IR and IS biotype at late growth stages in noncompetitive conditions and similar growth of IR and IS biotypes under competitive conditions indicated no competitive advantage from imazethapyr resistance.

Field studies were conducted in 1995 and 1996 to determine the rate response of velvetleaf seedling survival, seed production, and shoot biomass to postemergence herbicides in corn and soybean. Dicamba and imazethapyr were applied to corn and soybean, respectively, at 1, ½, ¼, ⅛, 1/16, 1/32, and 0× labeled rates. Velvetleaf mature plant density was linearly related to seedling density, thus indicating that seedling survival was not density dependent, even after seedling densities exceeded 150 plants m−2. Seedling survival as influenced by herbicide was described by a dose–response curve in corn and soybean. In corn, seedling survival ranged from 0 to 48% across herbicide treatments and years. Seedling survival was greater at the ½× or lower herbicide rates than at the 1× rate. In soybean, maximum seedling survival was 61 and 14% in 1995 and 1996, respectively, and minimum seedling survival was less than 2% in each year. Seedling survival was less in 1996 than in 1995 because velvetleaf was infected with wilt in 1996. In soybean, seedling survival was 20 times greater when treated with herbicides at the ½× rate than when treated at the 1× rate in 1995, but seedling survival was similar when herbicides were applied at 1, ½, ¼, and ⅛× rates in 1996. Velvetleaf fecundity (seeds per plant) was dependent on mature plant density in 1995 but was density independent in 1996. Fecundity as influenced by herbicide was described by dose–response curves in corn each year and in soybean in 1995. In 1995, velvetleaf treated with herbicides at ½× and ¼× rates produced 20 to 30 times more seed per square meter than when treated with herbicides at the 1× rate. Differences in seed per square meter were exaggerated by high densities of velvetleaf. Seed per square meter did not differ between velvetleaf treated with herbicides at 1× or ½× rates in corn or soybean in 1996. Wilt infection of velvetleaf in 1996 was the likely cause of differences in herbicide performance between years. Herbicides at reduced rates were not effective at limiting seedling survival and seed production compared to 1× rates in the absence of wilt. As a result, long-term management of velvetleaf with herbicides at reduced rates likely will be difficult, especially in areas with high densities, unless integrated with other management practices.

The objectives of this study were to model the influence of herbicides, wilt disease, and mechanical treatments on velvetleaf population dynamics, annualized net return (ANR), and economic optimum threshold (EOT) in a 20-yr rotation involving alternate years of corn and soybean. Mechanical treatments were interrow cultivation in corn and rotary hoeing in soybean. Herbicides at a quarter (¼×) rate or lower did not reduce velvetleaf seed banks without mechanical treatments in the absence of wilt. Herbicides at full (1×) and half (½×) rates decreased velvetleaf seed banks 95% within 6 and 20 yr, respectively, when there was no wilt. Herbicides at ½× rates with mechanical treatments reduced the seed bank 95% in only 10 yr, but mechanical treatments did not increase the rate of seed bank decline with 1× rates. Wilt infection had to occur annually to reduce velvetleaf seed banks as effectively as herbicides at 1× rates alone. ANR was maximized with herbicides at reduced rates, even though they were not as effective at reducing seed banks as were 1× rates. The herbicide rate required to maximize ANR increased as the initial velvetleaf seed bank density increased. Mechanical treatments and wilt decreased the herbicide rate required to maximize ANR. In fact, wilt infection increased the ANR of herbicides at reduced rates. The EOT was 0.55 and 0.4 seedlings m−2 when velvetleaf was managed with herbicides at 1× and ½× rates, respectively. Mechanical treatment had no effect on EOT, but wilt increased the EOT. Herbicides at reduced rates should only be used to manage velvetleaf in fields with a low seed bank density when integrated with mechanical treatments or when the field has a history of wilt. Herbicides should be used at 1× rates when fields have a large velvetleaf seed bank and when integrated management practices are not used.

The relative competitive abilities of yellow starthistle accessions that are resistant (R) and susceptible (S) to picloram were compared using a replacement series experiment. With no herbicide treatment, total shoot dry weights at vegetative and early reproductive stages of plant growth were similar for the two accessions, although S plants accumulated more total shoot dry weight by the late reproductive stage, mainly as a result of a greater contribution of vegetative growth. Without herbicide, relative yield of total biomass or reproductive structures did not differ from theoretical competitive equivalence at any accession ratio, thereby indicating that interaccession interference was similar. For picloram-treated plants, R plants accumulated more total, vegetative, and reproductive dry weight than did S plants at the early and late reproductive stages, and there was no difference between S and R plants at the vegetative growth stage. Seed production by R plants was 10-fold greater than that observed in S plants, but seed size remained unchanged, regardless of accession ratio. With herbicide present, the relative yield of S plants differed from theoretical competitive equivalence as S:R accession ratios decreased, but relative yield of R plants did not. Therefore, only in the presence of picloram will R plants have a competitive advantage over S plants. Some of the progeny from mixed populations of S and R plants that were cross-pollinated, even at low R frequency (25%), expressed resistance to picloram.

Three models that empirically predict crop yield from crop and weed density were evaluated for their fit to 30 data sets from multistate, multiyear winter wheat–jointed goatgrass interference experiments. The purpose of the evaluation was to identify which model would generally perform best for the prediction of yield (damage function) in a bioeconomic model and which model would best fulfill criteria for hypothesis testing with limited amounts of data. Seven criteria were used to assess the fit of the models to the data. Overall, Model 2 provided the best statistical description of the data. Model 2 regressions were most often statistically significant, as indicated by approximate F tests, explained the largest proportion of total variation about the mean, gave the smallest residual sum of squares, and returned residuals with random distribution more often than Models 1 and 3. Model 2 performed less well based on the remaining criteria. Model 3 outperformed Models 1 and 2 in the number of parameters estimated that were statistically significant. Model 1 outperformed Models 2 and 3 in the proportion of regressions that converged on a solution and more readily exhibited an asymptotic relationship between winter wheat yield and both winter wheat and jointed goatgrass density under the constraint of limited data. In contrast, Model 2 exhibited a relatively linear relationship between yield and crop density and little effect of increasing jointed goatgrass density on yield, thus overpredicting yield at high weed densities when data were scarce. Model 2 had statistical properties that made it superior for hypothesis testing; however, Model 1's properties were determined superior for the damage function in the winter wheat–jointed goatgrass bioeconomic model because it was less likely to cause bias in yield predictions based on data sets of minimum size.

Field studies were conducted to determine how the spatial arrangement of weed populations influences interspecific competition. We studied the influence of regular, random, and clumped distributions of barnyardgrass on growth and yield of direct-seeded tomato planted at different densities. Increasing aggregation increased intraspecific competition in barnyardgrass. At the same time, interspecific competition experienced by tomato from barnyardgrass decreased. Differences in the amount of shading of the tomato canopy by barnyardgrass contributed to yield loss differences for the various spatial arrangements. Clumped barnyardgrass caused significantly less average shading than barnyardgrass in regular or random arrangements. At a typical planting density of 10 tomato plants m−1 of row, yield losses ranged from 10 to 35% (1993) or 8 to 50% (1994) when competing with a clumped arrangement of barnyardgrass. At the same tomato density, yields were reduced from 20 to 50% (1993) or 11 to 75% (1994) for the regular and random arrangements for the same barnyardgrass densities. Predicted single-season economic threshold densities for barnyardgrass at a typical tomato planting density of 10 plants m−1 would be one barnyardgrass plant per 25, 19, or 15 m of crop row, respectively, for regular, random, and clumped spatial distributions.

Barnyardgrass was grown at densities of 0, 0.25, 0.5, 1, 2, 5, and more than 50 plants m−1 of tomato crop row in either a regular, random, or clumped pattern. Tomato was established at 0, 5, 10, or 20 plants m−1 of crop row in a regular pattern. Crop density and weed density or spatial arrangement had little effect on phenological development of barnyardgrass. In the absence of tomato, barnyardgrass was estimated to produce over 400,000 seeds plant−1 when not subjected to intraspecific competition (0.25 plants m−1 density), decreasing to about 10,000 seeds plant−1 when weed density exceeded 50 plants m−1 of row. Differences in seed production between plants in the regular and random spatial arrangements were minor, but the clumped distribution resulted in 30 to 50% reduction in seed production at weed densities between 1 and 5 plants m−1 of row. Tomato reduced barnyardgrass seed production. The magnitude of the reduction depended on both tomato density and barnyardgrass density. In the absence of tomato, barnyardgrass produced over 200,000 seeds m−2 in 1993 and over 500,000 seeds m−2 in 1994 at 5 plants m−1 of row. Production was almost 700,000 seeds m−2 when the weed density exceeded 50 plants m−1 of row. Barnyardgrass seed production at the single-season economic threshold density in tomato was sufficient to maintain the seedbank at a level that would mandate high levels of weed control in subsequent crops. Because of the high fecundity of barnyardgrass, our experiments suggest that stopping seed production is the best long-term management strategy for the weed. Spatial arrangement of the weed, at the scale used in these studies, would not be a factor in establishing long-term management guidelines based on weed population biology.

Field experiments were carried out in northern Greece during 1994, 1995, and 1996 to study the effect of nitrogen fertilization on competition between sterile oat and wheat, barley, and triticale. Dry weight of all crops was not affected until early March by sterile oats (110 plants m−2), but wheat and triticale dry weight were significantly reduced by sterile oats competition after that time. Grain yield of both wheat and triticale was equally reduced by 61% due to the presence of sterile oats, whereas the reduction for barley grain yield was 9%. Nitrogen fertilization (150 kg N ha−1) slightly increased yield of all crops grown without weed competition compared to the control (0 kg N), whereas the same treatment increased sterile oats dry weight as well as its competitive ability against wheat and triticale. Split application of nitrogen (50 kg N ha−1 before planting and 100 kg N ha−1 in early March) caused a slightly higher increase in sterile oats dry weight compared to the control or one application (150 kg N ha−1) before planting, when grown with wheat and triticale. However, dry weight of sterile oats grown with barley was severely reduced by the interference of the crop. Total nitrogen content of all crop plants grown without sterile oats increased with nitrogen fertilization compared to the control. However, total nitrogen in crop plants grown with sterile oats was reduced compared to the weed-free control; percent reduction was greater in plants grown in plots treated with nitrogen than in the control. These results indicate that barley could be used for limiting sterile oats interference in areas where winter cereals are grown; time of nitrogen application could also be used for a slight reduction of sterile oats competitive ability against wheat or triticale.

Field studies at three sites and growth chamber experiments were conducted to determine the reproductive potential, flower phenology, seed viability and germination, and overall seedbank longevity of yellow starthistle in the Central Valley of California. At the three study sites, seedheads contained an average of between 65 and 83 achenes. Overall, 85% of the achenes were the interior pappus-bearing type, and the remaining 15% were the outer nonpappus-bearing type. Germinable seed did not initially develop until the late corolla senescence stage 8 d after flower initiation. Seed germination and viability 1 wk after dispersal were similar (86 and 91%, respectively). Comparison in flower phenology in 1996 and 1997 indicated that development from initial anthesis to achene dispersal more closely corresponded to days, rather than thermal units. In the field, germinable seed was produced when more than 2% of the total seedheads had initiated anthesis. To minimize seed production with late-season control methods, such as prescribed burning, mowing, or herbicide treatment, management strategies should be timed before the plant population has advanced beyond the 2% flower initiation stage. Over 84% of the seed germinated under growth chamber conditions 1 wk after seedheads reached the dispersal stage. This indicates that most yellow starthistle seed had little or no after-ripening requirements. In a field experiment, yellow starthistle seed germination corresponded to seasonal rainfall. A total of 44 and 39% of the pappus-bearing and nonpappus-bearing seed, respectively, germinated after one growing season. Of seed recovered from the soil after the first growing season, 88 and 81% of the pappus-bearing and nonpappus-bearing seed, respectively, was either damaged or degraded. From projected values based on recovered and germinated seed, it was estimated that over 97% of the total seed was removed from the soil seedbank after two growing seasons. These findings should assist land managers in developing long-term yellow starthistle management strategies.

Field experiments were conducted in 1998 and 1999 at Five Points, CA, in the San Joaquin Valley under irrigated conditions to study competition between four commonly grown tomato cultivars and velvetleaf and to identify cultivar characteristics associated with greater tolerance to velvetleaf. The effect of velvetleaf competition varied with both year and tomato cultivar. When grown with 5 velvetleaf plants m−1 of row, marketable yield of tomato was reduced 8% in 1998 and 60% in 1999 for cultivar H8892 and 58% in 1998 and 80% in 1999 for cultivar H9661, compared to cultivars grown in monoculture. Across velvetleaf densities, height of tomato cultivars was not reduced compared to that of cultivars grown in monoculture. However in 1999, canopy width of tomato cultivars grown with velvetleaf was less than that of cultivars grown in monoculture. At early stages of growth, the leaf area index of tomato cultivars grown with velvetleaf was less than that of cultivars grown in monoculture. Crop growth rate and aboveground dry biomass of tomato cultivars grown with velvetleaf were generally less than those of cultivars grown in monoculture. Yield loss at high weed density was similar among cultivars, whereas yield loss at low weed density varied among cultivars. Cultivar tolerance to velvetleaf varied with year. However, cultivar H8892 had low yield loss and cultivar H9661 high yield loss at low weed density in 1998 and 1999. For cultivar H8892, leaf area expansion rate was also among the greatest for both years.

Research was initiated to determine the periodicity of emergence, seasonal plant growth and reproductive potential, and response to selected preemergence (PRE) and postemergence (POST) herbicides for burcucumber. Under Indiana conditions, burcucumber germinated from late April to October and was stimulated by periodic rainfall. Relative growth rates of plants were greatest up to 10 wk after establishment and declined when flowering was initiated. Without competition, early spring (May)–established burcucumber plants attained a fresh weight of up to 86 kg and produced almost 80,000 seeds. With later establishment, less biomass and and a smaller number of seeds were produced. Seedlings emerging up to mid-August produced germinable seed prior to frost, thus indicating that season-long control strategies are needed to minimize reproduction. A PRE application of atrazine, metribuzin plus chlorimuron, or linuron plus chlorimuron provided greater than 90% visual control up to 8 wk after treatment (WAT). Greater than 80% visual control was obtained with POST applications of glyphosate or with combinations of glyphosate plus dicamba or glyphosate plus 2,4-D; chlorimuron, metribuzin plus chlorimuron; or paraquat. Both imazaquin and bentazon provided less than 70% control. Results indicate that burcucumber displays rapid development with periodic germination throughout the growing season and is capable of producing large amounts of plant biomass and seed.

In studies conducted from 1991 through 1994, researchers investigated the effects of several crop rotations and herbicide programs on crop yield and populations of common lambsquarters, common ragweed, Amaranthus spp., and jimsonweed at two sites. Crop rotations included continuous corn, continuous soybean, corn–soybean, and corn–tomato–soybean, and herbicide programs were the split-plots and included continuous use of acetolactate synthase (ALS)–inhibitor herbicides, continuous use of non–ALS-inhibitor herbicides, annual rotations between ALS- and non–ALS-inhibitor herbicides, combinations of ALS- and non–ALS-inhibitor herbicides in the same year, and no herbicide. Weed control and weed populations generally were affected by an interaction between crop rotations and herbicide programs. After 4 yr, common lambsquarters control was lowest, and populations were highest where fomesafen was used alone for four consecutive years or in rotation with other herbicides. Although common ragweed populations were low at site 2, control at both sites was generally lowest from treatments that included only ALS-inhibitor herbicides. Common ragweed populations were highest at site 1 in 1992 and 1993 following continuous applications of ALS-inhibitor herbicides. Jimsonweed populations were also low at site 2, but control at site 1 in tomato was low. Jimsonweed control from fomesafen and the combination of butylate plus atrazine in soybean and corn, respectively, was variable. Amaranthus spp. populations decreased as the study progressed, and in 1993, control was over 90% from all treatments, except in the case of the treatment combining butylate plus atrazine. Corn and soybean yields varied with year and site, and yields of these crops and tomato were related to rainfall and weed control.

A population model of itchgrass was developed for a typical corn–based cropping system in the Pacific coastal region of Costa Rica. Field experiments were conducted to quantify density-dependent seedling mortality and fecundity. Additional information required for the model was obtained from the literature. Effect of control methods on itchgrass density—including a leguminous cover crop (velvetbean), a preemergence herbicide (pendimethalin), and classical biocontrol with the head smut—alone and in combination, were investigated using the model. According to model results, the cover crop planted at high and low densities between corn rows was highly efficient, reducing the initial itchgrass density from 54 plants m−2 to 4 and 17 plants m−2, respectively. Associating velvetbean with corn solely in the first crop each year resulted in predicted itchgrass densities of 33 and 36 plants m−2 (at high and low cover crop planting densities, respectively). The improvement in corn yield from preemergence herbicide or biocontrol in addition to the cover crop was only modest. This indicated that if, in practice, the cover crop is as effective as predicted, an inexpensive control tactic such as biological control (provided that an infection rate of at least 50% can be achieved) should be given priority to prevent income losses.

Subterranean turions (tubers) of hydrilla lose viability when desiccated. Experimental data showed that freshly collected tubers had a moisture content between 50 and 60% and more than 90% viability. When desiccated, there was an approximate 2% increase in tuber mortality with each percent decline in moisture content. However under field conditions, the tuber bank within the exposed sediments of a northern Texas reservoir showed no decline in number or tuber viability throughout a 12-mo continuous drawdown. Apparently, the buried tubers were never subject to sufficient dessication to damage them. Finally, an experimental pond with an extensive hydrilla tuber bank was manipulated through six flood/drawdown cycles to determine the effects of short-term drawdowns on tuber survival and quiescence. Initially, the pond had a tuber bank of about 676 and 305 tubers m−2 in the shallow and deep zones, respectively. Although the tuber number was reduced to fewer than 15 to 30 tubers m−2 by these repetitive drawdowns, hydrilla tubers were not eradicated from the pond.

Rice by-products were evaluated in the greenhouse for herbicidal activity on various weeds and crops. Rice by-products and corn gluten meal (CG) were applied at 0, 125, 250, 500, and 750 g m−2 preemergence (PRE) and preplant incorporated (PPI). The efficacy of rice by-products and CG in reducing weed emergence and shoot weight of broadleaf species was in the order of medium-grain fatty rice bran (MF) > long-grain fatty rice bran (LF) > CG > defatted rice bran (DF) > long-grain hull (LH) > medium-grain hull (MH). For reducing grass emergence, MF = CG > LF > DF > LH > MH, and for shoot weight reduction, CG > MF > LF > LH > DF = MH. Palmer amaranth and ivyleaf morningglory were the most susceptible weeds (91 and 82%) followed by sicklepod, hemp sesbania, and prickly sida (65 to 70%). Velvetleaf was the most tolerant broadleaf weed. Grasses were not as susceptible to rice bran as broadleaf weeds. In general, MF was the best material for reducing weed emergence and its efficacy was not affected by application method. Cotton and corn were the most tolerant direct-seeded crops to MF (6% reduction in plant stand), and soybean, Italian ryegrass, tomato, and rice had intermediate tolerances (30 to 86% stand reduction). Mustard, cucumber, and lettuce were the most susceptible crops (71 to 98% reduction in plant survival). The minimum effective rate was 250 g m−2 MF PPI or PRE.