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Using Protein Electrophoresis to Investigate the Phylogeny of Velvetleaf (Abutilon theophrasti)

Published online by Cambridge University Press:  12 June 2017

Steven J. Stegink  and
Neal R. Spencer
Steven J. Stegink
Affiliation:
South. Weed Sci. Lab., Agric. Res. Serv., U.S. Dep. Agric., Stoneville, MS 38776
Neal R. Spencer
Affiliation:
South. Weed Sci. Lab., Agric. Res. Serv., U.S. Dep. Agric., Stoneville, MS 38776
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Abstract

Native Polyacrylamide gel electrophoresis (PAGE) and enzyme activity staining were used to identify possible progenitor species of velvetleaf (Abutilon theophrasti Medic. # ABUTH). Multiple forms of superoxide dismutase activity were observed in each of the plants surveyed. Three enzyme forms were common to all the species and bio types while one form was different in the velvetleaf biotypes compared to the other species. Multiple forms of peroxidase activity were also detected. The three velvetleaf biotypes possessed identical enzyme forms with minimal similarity to peroxidase forms found in the other species. Multiple forms of esterase activity separated as two nonoverlapping groups. A slowly migrating group was observed in all the velvetleaf biotypes and a more rapidly migrating group characterized the remaining Abutilon species. The results of this study indicated that the progenitors of velvetleaf were not among the species surveyed and suggested that the progenitors may no longer be extant.

Keywords

ChemotaxonomyAbutilon bidentatumAbutilon fruticosumAbutilon graveolensAbutilon guineenseAbutilon hirtumAbutilon indicumAbutilon mauritianumAbutilon muticumAbutilon pannosumAbutilon umb elliflorum
Type
Weed Biology and Ecology
Information
Weed Science , Volume 36 , Issue 2 , March 1988 , pp. 172 - 175
Copyright
Copyright © 1988 by the Weed Science Society of America 

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References

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