A pine kraft lignin (PC940C) controlled the release of 14C-chloramben (3-amino-2,5-dichlorobenzoic acid), 14C-metribuzin [4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one], and 14C-alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide] as measured by water leaching in soil columns. As more PC940C was used, a concentration effect was found with more of the three herbicides being retained in the top portion of the soil columns. Combinations of alachlor and metribuzin applied with PC940C did not alter the retention in the top portion of the soil columns of either 14C-metribuzin or 14C-alachlor compared to each herbicide applied with PC940C alone. This would suggest that, at the rate of PC940C used, there was no competition between either herbicide for the controlled-release function of PC940C. The percentage of 14C-chloramben, 14C-metribuzin, and 14C-alachlor retained by PC940C in the top portion of the columns was very similar. Combinations of metribuzin and alachlor without PC940C reduced the mobility of both 14C-metribuzin and 14C-alachlor. Finally, 3H-PC940C was found to be immobile in soil columns leached with water.

The response of potato (Solanum tuberosum L. 'Superior’) to glyphosate [N-(phosphonomethyl)glycine] at rates between 0.28 and 2.24 kg ae/ha was determined in four environments: 24/13 or 13/4 C temperature regimes with light levels of 650 ± 53 or 320 ± 45 μE · m−2 · S−1. Light level did not affect phytotoxicity ratings or shoot fresh weight following glyphosate application. Greater phytotoxicity and reduced fresh weight accumulation were observed from glyphosate at rates above 0.56 kg/ha in a high-temperature regime than for comparable plants grown at low temperature. Studies of 14C-glyphosate uptake and translocation indicated that the differences in phytotoxicity were due to less glyphosate absorption at low temperature and not a shift in distribution patterns within the plant.

Experiments were conducted to determine 14C absorption, translocation, and metabolism by pitted morningglory (Ipomoea lacunosa L. # IPOLA) and ivyleaf morningglory [Ipomoea hederacea (L.) Jacq. # IPOHE] foliarly treated with 14C-acifluorfen [5-(2-chloro-4-(trifluoromethyl)phenoxy)-2-nitrobenzoic acid] or 14C-lactofen [(±)-2-ethoxy-1-methyl-2-oxoethyl-5-(2-chloro-4-(trifluoromethyl)phenoxy)-2-dinitrobenzoate]. Seventy-one to 84% of applied 14 C-acifluorfen was recovered in leaf water wash of ivyleaf morningglory compared to 32 to 46% from pitted morningglory. Sixty-four percent of applied 14C-lactofen was recovered in leaf water wash 96 h after treatment in both morningglory species. Thirty-five to 37% more 14C from applied 14C-acifluorfen was found in pitted morningglory treated leaves than ivyleaf morningglory treated leaves. Less than 28% of applied 14C-lactofen was extracted from treated leaves of either morningglory species. Translocation and metabolism of 14C-acifluorfen and 14C-lactofen were minimal in both morningglory species.

Characteristics of glyphosate [N-(phosphonomethyl)glycine] uptake for as long as 3 days by plant cells were studied using suspension-cultured potato (Solanum tuberosum L. cv. ‘Red Pontiac’ and S. brevidens Phil.) cells. An experimental system was developed wherein uptake was assayed in a reduced salt medium that minimized cell growth yet maintained cell viability. Glyphosate uptake was nonlinear over time; an immediate rapid uptake phase was followed by uptake at a decreased rate that continued through 72 h. Glyphosate uptake was the same in both an experimental uptake medium (reduced salt and sucrose, no hormones) and in the complete growth medium used for routine cell growth. The pattern of glyphosate uptake was similar with both Red Pontiac and S. brevidens cells, though uptake was greater in Red Pontiac cells. Glyphosate uptake was not inhibited by 1 mM CaSO4, MgSO4, MnSO4, or ZnSO4. Rather, uptake was inhibited by the absence of salts at 8 h, and ZnSO4 and MnSO4 increased uptake. Similarly, increasing concentrations (0 to 5 mM) of CaSO4 and CaCl2 did not inhibit uptake, whereas the absence of either salt of Ca2+ decreased uptake. Increasing concentrations of (NH4)2 SO4 (0 to 150 mM) did not affect glyphosate uptake, but (NH4)2 SO4 at 50 mM and above was toxic to the cells.

Experiments were conducted on uptake and translocation of butyl ester of 14C-fluazifop {(±)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy] propanoic acid} in green foxtail [Setaria viridis (L.) Beauv. # SETVI]. Absorption of 14C-fluazifop reached 67.6% 12 h after treatment with the petroleum oil additive compared to 44.0% when applied alone. Maximum 14C-fluazifop absorption detected was 74.2% after 48 h with the oil additive compared to 58.6% after 72 h when applied alone. Translocation of 14C-fluazifop was greater when applied with the oil additive than alone, but translocation did not increase after 12 h. Absorption and translocation of 14C-fluazifop by green foxtail was greater when applied with the oil additive than alone in all environments. The oil additive overcame the reduced absorption of 14C-fluazifop applied alone with low temperature (20 C), relative humidity (40%), and soil fertility (0.25× strength Hoagland's solution), but not the reduced absorption and translocation with an increase in stress to −10 bars. 14C-fluazifop lost as vapors from the treated green foxtail leaf was 4.1% when applied alone compared to 0.6% when applied with an oil additive.

Volunteer corn (Zea mays L. # ZEAMX) is a troublesome weed in soybeans [Glycine max (L.) Merr.] grown in the Corn Belt. Field studies were conducted in 1983 and 1984 at Champaign, IL, to determine the soybean growth and yield parameters affected by volunteer corn interference. Soybean yield was inversely and linearly related to volunteer corn clump density, with yield losses reaching 25% at 5380 clumps/ha (10 plants/clump). Volunteer corn clumps of 1, 4, 7, and 10 plants reduced soybean yields by 6, 16, 21, and 22%, respectively, at a density of 5380 clumps/ha. Treatments of either the butyl ester of fluazifop {(±)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy] propanoic acid} or glyphosate [N-(phosphonomethyl)glycine] were applied at various dates between 2 and 12 weeks after soybean emergence (WAE) to a volunteer corn infestation of 5380 clumps/ha (10 plants/clump). Volunteer corn caused soybean yield losses of 2, 6, 12, 19, and 27% when herbicides were applied at 2, 4, 6, 8, and 10 WAE, respectively. Volunteer corn interference reduced soybean seed yield, plant dry weight, pod number, seed number, leaf fresh weight, and leaf area index, within 40 cm of a clump of 10 volunteer corn plants. A clump of 10 volunteer corn plants reduced soybean pod number/plant within a radius of 86 cm.

Seedling growth of soybean [Glycine max (L.) Merr. ‘Forrest’], common cocklebur (Xanthium strumarium L. # XANST), and tall morningglory [Ipomoea purpurea (L.) Roth. # PHBPU] was compared in field studies. Root growth was determined by measuring roots restricted to the inside of a permeable membrane that contained no soil. Leaf area duration and net assimilation rate between 4 and 9 weeks were greatest for common cocklebur compared to the other species. At 9 weeks, shoot and root dry weight and leaf area were greatest for common cocklebur compared to soybean and tall morningglory. Relative growth rate of common cocklebur roots was greater than the other species between 4 and 9 weeks. Tall morningglory had greater shoot length or height at 4 and 9 weeks and greater shoot or height elongation rate between 2 and 4 weeks, and 4 and 9 weeks than the other species.

Native Polyacrylamide gel electrophoresis (PAGE) and enzyme activity staining were used to identify possible progenitor species of velvetleaf (Abutilon theophrasti Medic. # ABUTH). Multiple forms of superoxide dismutase activity were observed in each of the plants surveyed. Three enzyme forms were common to all the species and bio types while one form was different in the velvetleaf biotypes compared to the other species. Multiple forms of peroxidase activity were also detected. The three velvetleaf biotypes possessed identical enzyme forms with minimal similarity to peroxidase forms found in the other species. Multiple forms of esterase activity separated as two nonoverlapping groups. A slowly migrating group was observed in all the velvetleaf biotypes and a more rapidly migrating group characterized the remaining Abutilon species. The results of this study indicated that the progenitors of velvetleaf were not among the species surveyed and suggested that the progenitors may no longer be extant.

Growth, development, and germination among jointed goatgrass (Aegilops cylindrica Host. # AEGCY) accessions collected from nine western states were not consistently different in field plots near Pullman, WA, or in the greenhouse. Germination percentage and rate of spikelet germination of all accessions produced in the field and greenhouse were consistently high, averaging about 64% and 5.2 (on a unitless scale of 0 to 7), respectively. The Montana accession germinated consistently less than the others. In a separate greenhouse study comparing only the accessions from Colorado and Nebraska, the accession from Colorado averaged 18, 16, and 15% greater net photosynthesis rate (Pn), transpiration rate (E), and stomatal density, respectively, and 21% lower diffusive resistance (rl) than did the accession from Nebraska. However, growth parameters of the Nebraska accession averaged 7 to 35% greater than for the Colorado accession. Overall, the germination, growth, CO2 fixation, and water use among accessions appeared to be similar when jointed goatgrass was grown under Pacific Northwest conditions.

Dyers woad (Isatis tinctoria L. # ISATI) has greatly expanded on rangelands in the Intermountain region. Herbicidal and tillage controls are not feasible on rangelands. Better knowledge of the biology of this species could help in the development of biological controls. We examined characteristics that could assist in this effort. Seed viability remained high and relatively stable, but germination decreased over a 10-month period. The seed dispersal pattern of dyers woad was best described by a negative exponential model (log10 y = 1.92-0.02x; r2=0.60), where y equals seeds/m2 and x = distance from mother plant (cm). The root system of dyers woad is dominated by a taproot with some laterals in the upper 30 cm of the soil profile. Survivorship of experimentally established populations monitored over 2 yr showed constriction at two stages: 1) germination and establishment, and 2) young rosette. The latter stage should be targeted for biological control.

Patterns of spread of 85 weed species alien to the northwestern U.S. were simulated using principal coordinate analysis on primarily herbaria-derived data. The primary pattern detected was a west-to-east migration of weeds. The point of introduction for most weeds following this pattern appeared to be near Portland, OR, an important shipping port in the past. An east-to-west migration represented a secondary pattern. The major cattle and wheat-cropping areas of east-central Montana were the apparent introduction points for weeds following this pattern. The grain and pulse region of eastern Washington represented the point of introduction of species following a third pattern of migration. These weeds subsequently spread southward to Utah and then laterally to the east and west. Historical weed migration patterns provide insight to probable points of origin and routes of currently spreading weeds as well as those that will spread in the future. Such insight may aid in the containment of future spreading weeds.

Field studies were conducted for 3 yr near Mead, NE, to evaluate the effectiveness of seed safeners CGA-92194 [N-(1,3-dioxalon-2-yl-methoxy)iminobenzeneacetonitrile], NA (1,8-naphthalic anhydride), and R-29148 (2,2-dimethyl-5-methyldichloroacetyloxazolidine) to reduce herbicide injury to big bluestem (Andropogon gerardii Vitman), indiangrass [Sorghastrum nutans (L.) Nash], intermediate wheatgrass [Agropyron intermedium (Host.) Beauv.], sideoats grama [Bouteloua curtipendula (Michx.) Torr.], and switchgrass (Panicum virgatum L.) from preplant-incorporated butylate [5-ethyl bis-(2-methylpropyl)carbamothioate] and metolachlor [2-chloro-N- (2-ethyl-6-methylphenyl) -N- (2-methoxy-l-methylethyl)acetamide], applied at 4.5 and 2.2 kg ai/ha, respectively. Big bluestem stands were satisfactory, regardless of herbicide or safener treatment, although stands were reduced by NA treatment with either herbicide. Indiangrass stands varied by year, with protection from both herbicides by R-29148 in 1984 and by NA in 1985. All safeners reduced injury to intermediate wheatgrass from metolachlor and to a lesser extent from butylate; acceptable stands were obtained with metolachlor treatment when unsafened. Sideoats grama was nearly eliminated with either herbicide, regardless of safener. Switchgrass treated with NA produced stands two- to threefold higher than other safened or unsafened seed in metolachlor plots and equal to unsafened seed in the weeded control plots.

Application time did not greatly influence control of velvetleaf (Abutilon theophrasti Medik. # ABUTH) or common lambsquarters (Chenopodium album L. # CHEAL) in no-till corn (Zea mays L. ‘Pioneer 3747’) with fluorochloridone {3-chloro-4-(chloromethyl)-1-[3-(trifluoromethyl) phenyl]-2-pyrrolidinone}. Giant foxtail (Setaria faberi Herrm. # SETFA) control was reduced as much as 25% by 90 days after planting when fluorochloridone was applied early preplant rather than preemergence. Fluorochloridone at 0.8 kg/ha applied preplant or preemergence gave 83% or greater control of common lambsquarters and giant foxtail for the entire growing season. However, velvetleaf control with the same treatments was 61% or less. Fluorochloridone caused minimal corn injury. Greenhouse bioassay indicated that fluorochloridone may carry over and injure soybean [Glycine max (L.) Merr.] the year after application. Imbibition of fluorochloridone by seed of corn and giant foxtail did not reduce germination at concentrations up to 10-3 M. Giant foxtail seedling fresh weight was reduced 80% following imbibition of 10-5 M fluorochloridone. Corn seedling fresh weight was not reduced by imbibition of up to 10-4 M fluorochloridone.

Proso millet (Panicum miliaceum L. # PANMI) was controlled in corn (Zea mays L. ‘Pioneer 3747’) with postemergence-directed herbicides applied following a standard preplant-incorporated application of butylate [S-ethylbis(2-methylpropyl)carbamothioate] plus dichlormid (2,2-dichloro-N,N-di-2-propenylacetamide) plus cyanazine {2-[[4-chloro-6-(ethylamino)-1,3,5-triazin-2-yl]amino]-2-methylpropanenitrile}. Postemergence-directed applications of ametryn [N-ethyl-N′-(1-methylethyl)-6-(methylthio)-1,3,5-triazine-2,4-diamine], cloproxydim {(E,E)-2-[1-[[(3-chloro-2-propenyl)oxy]imino]butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one}, butyl ester of fluazifop {(±)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy] propanoic acid}, butyl ester of fluazifop-p {(R)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy] propanoic acid}, glyphosate [N-(phosphonomethyl)glycine], methyl ester of haloxyfop {2-[4-[[3-chloro-5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy]propanoic acid}, and sethoxydim {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one} in 40-cm-tall corn gave greater than 90% control of 5- to 15-cm-tall proso millet while causing little or no corn injury at selected rates. Season-long proso millet control was obtained with sethoxydim at rates as low as 56 g ai/ha with no visible corn injury. Unacceptable injury to 40-cm-tall corn occurred with all but the lowest rate of sethoxydim (56 g ai/ha) when a crop oil concentrate was used. Sethoxydim applied as a directed spray at rates up to 110 g ai/ha plus 1.25% (v/v) crop oil concentrate did not injure 60-cm-tall corn.

The efficacies of microencapsulated (ME) and emulsifiable concentrate (EC) formulations of alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide] and metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide] were compared in petri dish and soil bioassays. At equimolar concentrations, EC-alachlor inhibited shoot growth of barnyardgrass [Echinochloa crus-galli (L.) Beauv. # ECHCG], large crabgrass [Digitaria sanguinalis (L.) Scop. # DIGSA], and fall panicum (Panicum dichotomiflorum Michx. # PANDI) more than ME-alachlor. ME- and EC-metolachlor caused similar effects on the three grass weeds tested. ME- and EC-alachlor in combination caused effects on barnyardgrass shoot growth intermediate to results with either formulation alone. Air-drying and remoistening of alachlor-treated filter paper or soil before sowing barnyardgrass or fall panicum caused greater shoot length and fresh weight reduction than ME-alachlor that was not dried.

The growth regulator NAA was investigated to determine whether it would control creeping woodsorrel (Oxalis corniculata L. # CYPES) with no phytotoxicity to container ornamental species. Dosage, carrier volume, number of applications, and plant age and growth stage were examined as factors in the phytotoxicity of NAA. Creeping woodsorrel response to the ethyl ester formulation of NAA was linear for dosages up to 5.6 kg/ha; this rate resulted in about 80% injury in all experiments. NAA applied at 8.4 and 11.2 kg/ha achieved 99 and 100% control, respectively. Neither carrier volume nor split applications had any effect on phytotoxicity of NAA. In all experiments, phytotoxicity appeared to increase until around 2 to 3 weeks from application. Phytotoxicity to creeping woodsorrel was related to plant age; as plant age at the time of treatment increased, the level of injury decreased. When plants treated with NAA at rates up to 2.8 kg/ha were cut back to soil level, some regrowth occurred in all cases. Rates of NAA that controlled creeping woodsorrel (5.6 and 11.2 kg/ha) were not phytotoxic to several ornamental species.

Three herbicide treatments were evaluated for use under clear polyethylene mulch and three types of row covers (spunbonded polyester, double-slitted polyethylene, and perforated polyethylene). Treatments included combinations of bensulide {O,O-bis(1-methylethyl)-S-[2-[(phenylsulfonyl)amino]ethyl]phosphorodithioate} and naptalam {2-[(1-naphthalenylamino)carbonyl]benzoic acid}, and cinmethylin {exo-1-methyl-4-(1-methylethyl)-2-[(2-methylphenyl)methoxy]-7-oxabicyclo [2.2.1] heptane} alone and in combination with naptalam. In 1985 and 1986, all herbicide treatments controlled more than 95% of carpetweed (Mollugo verticillata L. # MOLVE) and large crabgrass [Digitaria sanguinalis (L.) Scop. # DIGSA], except for the combination of bensulide plus naptalam in 1986, which controlled only 73 to 85% of these weeds. In both years, all herbicide treatments reduced crop vigor at 3 weeks but not at 7 weeks after transplanting. Total yield was not different with any of the herbicide treatments in 1985 and 1986. However, in 1986, yields were greater from the bensulide plus naptalam treatment than for the weed-free control. Herbicide treatment did not affect early yields in 1985, but the bensulide plus naptalam treatment was greater than all others in 1986. The use of row covers did not increase herbicide-induced crop injury over that of clear mulch alone. Greenhouse studies indicated that soil-applied cinmethylin reduced muskmelon root and shoot weight. Foliar applications reduced muskmelon shoot weight.

The response of ‘Era’ (tolerant) and ‘Coteau’ (susceptible) hard red spring wheat (Triticum aestivum L.) to CGA-82725 2-propynyl ester of {2-[4- [(3,5-dichloro-2-pyridinyl)oxy] phenoxy] propanoic acid} was determined in field and greenhouse evaluations. Era wheat was most susceptible to CGA-82725 applied at early jointing through the boot stage. Coteau wheat was most susceptible at die two-leaf stage and between early jointing through spike emergence. Coteau was injured more than Era wheat as the CGA-82725 rate was increased from 0.14 to 0.28 kg ai/ha. Wheat grain yield reductions were greatest when CGA-82725 was applied at the early jointing through boot stage for both cultivars and averaged 73% of the control. Absorption and translocation of 14C-CGA-82725 were similar and increased over time regardless of cultivar. Most of the absorbed 14C remained in the treated leaf in both cultivars.

Laboratory and field research was conducted to determine the influence of winter wheat (Triticum aestivum L.) stubble on weed control and the dissipation of emulsifiable concentrate formulations (EC) of metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl) acetamide], alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide], and acetochlor [2-chloro-N-(ethoxymethyl)-N-(2-ethyl-6-methylphenyl)acetamide]; and microencapsulated formulations (ME) of alachlor and acetochlor. EC metolachlor provided better weed control than EC alachlor when applied 28 days before planting sorghum (Sorghum bicolor L.) and similar weed control when applied at 1 or 14 days before planting. This difference was attributed to more rapid EC alachlor dissipation. The order of relative herbicide persistence at 24 C and 33% (w/w) soil moisture in a silty clay loam was ME acetochlor = ME alachlor > EC metolachlor > EC acetochlor = EC alachlor. Acetanilide degradation was affected more by increasing temperature from 15 to 24 C than by increasing soil moisture from 15 to 33%. More herbicide was washed from overwintered than fresh straw in the first 1 cm of simulated rainfall. In the first simulated rainfall event, more herbicide was removed from straw that had been dry than wet at application, but the amount of herbicide remaining on dry and wet straw was similar after four events. Formulation generally did not affect herbicide wash-off.

Bentazon [3-(1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide] was evaluated for Canada thistle [Cirsium arvense (L.) Scop. # CIRAR] control and birdsfoot trefoil (Lotus corniculatus L. ‘Norcen’) tolerance. Bentazon applied in split applications controlled more Canada thistle than a single application of equal rate in both greenhouse and field studies. Bentazon applied four times reduced the level of total nonstructural carbohydrates (TNC) in the roots of both greenhouse- and field-grown Canada thistle and increased birdsfoot trefoil injury more than bentazon applied in a single application of equal rate. All bentazon treatments caused unacceptable injury when birdsfoot trefoil was grown for seed.