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Histology of Infection of Hydrilla verticillata by Macrophomina phaseolina

Published online by Cambridge University Press:  12 June 2017

Gary F. Joye  and
Rex N. Paul
Gary F. Joye
Affiliation:
Plant Pathol., U.S. Army Engineers, Waterways Exp. Stn., Vicksburg, MS
Rex N. Paul
Affiliation:
South. Weed Sci. Lab., U.S. Dep. Agric., Agric. Res. Serv., P.O. Box 350, Stoneville, MS 38776
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Abstract

Infection of Hydrilla verticillata by Macrophomina phaseolina was investigated using scanning and transmission electron microscopy. Sprigs of plants in petri plates were inoculated with suspensions of fungal hyphae. Samples of inoculated and noninoculated plants were taken over time. Fungal cells attached to lower epidermal cell walls but not the upper epidermal cell walls of leaves. In less than 40 h, penetration through the cell wall was completed and colonization of host cells was observed. Penetration of upper epidermis was limited to the cell wall adjacent to a lower epidermal cell. No penetration was observed through the outer cell wall of upper epidermis. Inhibition of penetration through the outer cell wall of the upper epidermis may be attributable to an osmiophilic layer below the cell wall. Disruption of the host cell walls and subsequent host cell death was preceded by massive colonization of the host by this pathogen.

Keywords

HydrillaHydrilla verticillata (L.f.) Royle #3 HYLLIBiocontrolaquatic plantshistopathology
Type
Special Topics
Information
Weed Science , Volume 40 , Issue 2 , June 1992 , pp. 288 - 295
Copyright
Copyright © 1992 by the Weed Science Society of America 

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References

Literature Cited

1. Ancibor, E. 1979. Systematic anatomy of vegetative organs of the Hydrocharitaceae. Bot J. Linean Soc. 78:237–266.CrossRefGoogle Scholar
2. Ammon, V., Wyllie, T. D., and Brown, M. F. Jr. 1974. An ultrastructural investigation of pathological alterations induced by Macrophomina phaseolina (Tassi) Goid in seedlings of soybean, Glycine max. (L.) Merrill. Physiol. Plant Pathol. 4:1–4.Google Scholar
3. Bhattacharya, G., Dhar, T. K., Bhattacharya, F. K., and Siddiqui, A. I. 1987. Mutagenic action of phaseolinone, a mycotoxin isolated from Macrophomina phaseolina . Aust. J. Biol. Sci. 40:349–353.Google Scholar
4. Bowes, G., Van, T. K., Garrard, L. A., and Haller, W. T. 1977. Adaptation to low light levels of hydrilla. J. Aquat. Plant Manage. 15:32–35.Google Scholar
5. Chan, Y. H. and Sackston, W. E. 1973. Nonspecificity of the necrosis-inducing toxin of Sclerotium bataticola . Can. J. Bot. 51:690–692.Google Scholar
6. Deshpande, K. S. and Wadje, S. S. 1978. Toxic metabolites of Rhizoctonia bataticola . Indian J. Exp. Biol. 16:137–138.Google Scholar
7. Dooris, G. M., Dooris, P. M., and Martin, D. F. 1988. Effect of a naturally occurring growth inhibitor on the ultrastructure of hydrilla. J. Aquat. Plant Manage. 26:72–73.Google Scholar
8. Gangopadhyay, S. and Wyllie, T. D. 1978. Effect of phenolic compounds on growth in vitro of Macrophomina phaseolina (Tassi) Goid. Indian J. Microbiol. 18:13–15.Google Scholar
9. Goel, S. K. and Mehrotra, R. S. 1974. Production of pectolytic and cellulolytic enzymes by Macrophomina phaseolina in vitro and in vivo. Indian Phytopathol. 27:171–177.Google Scholar
10. Goodman, R. N., Kiraly, Z., and Wood, K. R. 1986. The Biochemistry and Physiology of Plant Disease. Univ. of Missouri Press, Columbia. 433 pp.Google Scholar
11. Gunning, B. and Pate, J. 1969. “Transfer Cells” Plant cells with wall ingrowths, specialized in relation to short distance transport of solutes — their occurrence, structure and development. Protoplasma 68:107–133.Google Scholar
12. Joye, G. F. 1990. Biocontrol of Hydrilla verticillata with the endemic pathogen Macrophomina phaseolina . Plant Dis. 74:1035–1036.Google Scholar
13. Joye, G. F. 1990. Biological control of aquatic weeds with plant pathogens. Pages 155–173 in Hoagland, R. E., ed. Microbes and Microbial Products as Herbicides. ACS Symp. Ser. 439.Google Scholar
14. Ledingham, J. M. and Simpson, F. O. 1972. The use of phenylenediamine in the block to enhance osmium staining for electron microscopy. Stain Technol. 47:239–243.Google Scholar
15. Madkour, M. A. and Aly, M. H. 1981. Cell wall degrading enzymes produced during pathogenesis of Macrophomina phaseolina on strawberry plants. Phytopathol. Z. 100:36–43.Google Scholar
16. Pendland, J. 1976. Ultrastructure of Hydrilla verticillata (L.F.) Boyle and related physiological implications, PhD. Thesis, Univ. Florida, Gainesville, FL. 106 pp.Google Scholar
17. Pendland, J. 1979. Ultrastructural characteristics of Hydrilla leaf tissue. Tissue and Cell 11:79–88.Google Scholar
18. Pieterse, A. H. 1981. Hydrilla — a review. Abstracts on Tropical Agriculture 7:9–34.Google Scholar
19. Ramasami, R. and Shanmugam, N. 1976. Studies on pectolytic and cellulolytic enzymes of Rhizoctonia bataticola in vitro and in vivo. Indian Phytopathol. 29:385–388.Google Scholar
20. Robson, T. H. 1976. A review of the distribution of aquatic weeds in the tropics and subtropics. Pages 25–30 in Aquatic Weeds in S. E. Asia, Proc. Reg. Sem. on noxious aquatic vegetation New Delhi. W. Junk, The Hague.Google Scholar
21. Saha, S. C., Dube, S., Sanyal, A., Dube, D. K., and Sinha, S. N. 1979. Production of extra-cellular enzymes by jute pathogenic fungus Macrophomina phaseolina . Curr. Sci. 48:484–485.Google Scholar
22. Sanyal, A., Saha, S. C., Kundu, R. K., Sinha, S. N., and Dube, D. K. 1981. Induction of carboxymethyl cellulose in Macrophomina phaseolina . Folia Microbiol. 26:83–88.Google Scholar
23. Short, G. E., Wyllie, T. D., and Ammon, V. D. 1978. Quantitative enumeration of Macrophomina phaseolina in soybean tissues. Phytopathology 68:736–741.CrossRefGoogle Scholar