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Serum C3c and C4c concentrations and adenosine deaminase activity of children with cystic fibrosis: preliminary study

Published online by Cambridge University Press:  12 May 2008

P. Perris ,
M. S. Feliu ,
S. Barbeito ,
I. Strasnoy ,
M. Ferraro  and
N. Slobodianik
P. Perris
Affiliation:
Department of Nutrition, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina
M. S. Feliu
Affiliation:
Department of Nutrition, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina
S. Barbeito
Affiliation:
Nutrition Service, Pedro Elizalde Hospital, Buenos Aires, Argentina
I. Strasnoy
Affiliation:
Nutrition Service, Pedro Elizalde Hospital, Buenos Aires, Argentina
M. Ferraro
Affiliation:
Nutrition Service, Pedro Elizalde Hospital, Buenos Aires, Argentina
N. Slobodianik
Affiliation:
Nutrition Service, Pedro Elizalde Hospital, Buenos Aires, Argentina
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Abstract

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Type
1st International Immunonutrition Workshop, Valencia, 3–5 October 2007, Valencia, Spain
Information
Proceedings of the Nutrition Society , Volume 67 , Issue OCE1: 1st International Immunonutrition Workshop, Valencia, 3–5 October 2007, Valencia, Spain , May 2008 , E59
Copyright
Copyright © The Authors 2008

Nutrition is a critical component of the management of cystic fibrosis (CF), and nutritional status is directly associated with both pulmonary status and survival(Reference Dodge and Turck1). Previous results have shown that several serum fractions (apoB, transferrin) related to the nutritional status of children with CF are diminished(Reference Perris, Barbeito, Strasnoy, Ferraro, Ramos and Slobodianik2). The present preliminary study measured in the same group of children specific serum proteins associated with the immune system and the activity of adenosine deaminase (ADA), an enzyme associated with T lymphocytes(Reference Kurashige, Akusawa, Yoshida, Teshima, Kodama and Mitsuhashi3, Reference Feliu and Slobodianik4). Sixteen children of both genders with CF, between 5 months and 11 years of age, were evaluated between September 2005 and February 2007,with assistance from the Nutrition Service. Samples of whole blood were collected from fasting patients. C3 and C4 complement fractions (C3c, C4c) were measured by single radial immunodiffusion techniques using commercially-available kits (Diffuplate; Biocientifica, Buenos Aires, Argentina)(Reference Mancini, Carbonara and Heremans5). The activity of ADA was determined by the method of Giusti & Galanti(Reference Bergmeyer6). The results were compared with reference values obtained from healthy children matched for age and gender.

Mean values were significantly different from those for the reference group: *0.001<P<0.02.

Significantly decreased C3c and C4c values with a concomitant increase in the activity of ADA were observed in patients with CF. The increase in the activity of ADA would be an alternative mechanism to avoid the accumulation of high levels of deoxynucleotides, which would be toxic for T-cell lymphocytes(Reference Feliu and Slobodianik4). These preliminary results suggest that the immune system, evaluated using serum levels of C3c and C4c and the activity of ADA, is altered. Specific nutritional support should be established and adjusted to individual needs.

Partially supported by University of Buenos Aires (Grant B-060).

References

1. Dodge, JA & Turck, D (2006) Best Pract Res Clin Gastroenterol 20, 531–556.CrossRefGoogle Scholar
2. Perris, P, Barbeito, S, Strasnoy, I, Ferraro, M, Ramos, O & Slobodianik, N (2007) Proceedings of the AACC Annual Meeting. Poster-B-56. http://www.aacc.org/AACC/events/ann_meet/annual2007/Google Scholar
3. Kurashige, S, Akusawa, X, Yoshida, T, Teshima, C, Kodama, K & Mitsuhashi, S (1982) Microbiol Immunol 26, 87–92.CrossRefGoogle Scholar
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