Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-26T00:55:09.529Z Has data issue: false hasContentIssue false
  • English
  • Français

Variation in class-specific humoral immune responses of different mouse strains to microfilariae of Dipetalonema viteae

Published online by Cambridge University Press:  06 April 2009

N. M. Almond ,
M. J. Worms ,
W. Harnett  and
R. M. E. Parkhouse
N. M. Almond
Affiliation:
Divisions of Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
M. J. Worms
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
W. Harnett
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
R. M. E. Parkhouse*
Affiliation:
Divisions of Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
*
†Reprint requests to Dr R. M. E. Parkhouse.
Get access Rights & Permissions [Opens in a new window]

Summary

The class-specific antibody responses of 3 strains of mice (C57/Bl10, BALB/C and CBA/N) known to vary in their ability to control the microfilaraemia which follows the subcutaneous transplantation of adult female Dipetalonema viteae has been investigated. The 3 mouse strains showed significant variation (a) in total levels of immunoglobulins and (b) in ability to recognize individual radio-isotope-labelled antigens as measured by coprecipitation. Within each mouse strain it was noted that antigens could vary with respect to the nature of the isotype of the antibody response which they elicited. Furthermore, by comparing results obtained from class-specific coprecipitation with surface ELISA it was found that a similar variation between responses to individual epitopes was also likely. No differences were observed in the humoral response of the 3 mouse strains which could explain the known resistance of the C57/B110 strain; reasons for this are discussed.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 3 , December 1987 , pp. 559 - 568
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Almond, N. M. & Parkhouse, R. M. E. (1986). Immunoglobulin class specific responses to biochemically defined antigens of Trichinella spiralis. Parasite Immunology 8, 391–406.CrossRefGoogle ScholarPubMed
Almond, N. M., Parkhouse, R. M. E., Chapa-Ruiz, M. R. & Garcia-Ortigoza, E. (1987). The response of humans to surface and secreted antigens of Trichinella spiralis. Tropical Medicine and Parasitology 37, 381–4.Google Scholar
Chandrashekar, R., Rao, U. R., Rajasekariah, G. R. & Subrahmanyam, D. (1984). Separation of viable microfilariae free of blood cells on Percoll gradients. Journal of Helminthology 58, 69–70.CrossRefGoogle ScholarPubMed
Haque, A., Worms, M. J., Ogilvie, B. M. & Capron, A. (1980). Dipetalonema viteae: microfilariae production in various mouse strains and in nude mice. Experimental Parasitology 49, 398–404.CrossRefGoogle ScholarPubMed
Mancini, G., Carbonara, A. O. & Heremans, J. F. (1965). Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235–54.CrossRefGoogle ScholarPubMed
Neilson, J. J. M., Crandall, C. A & Crandall, R. B. (1981). Serum immunoglobulin and antibody levels and the passive transfer of resistance in hamsters infected with Dipetalonema viteae. Acta Tropica 38, 309–18.Google ScholarPubMed
Ortega-Pierres, G., Chayen, A., Clark, N. W. T. & Parkhouse, R. M. E. (1984). The occurrence of antibodies to hidden and exposed determinants of surface antigens of Trichinella spiralis. Parasitology 88, 359–69.CrossRefGoogle ScholarPubMed
Ottesen, E. A., Neva, F. A., Paranjape, R. S., Tripathy, S. P., Thiruvengadam, K. V. & Beaven, M. A. (1979). Specific allergic sensitisation to filarial antigens in tropical eosinophilia syndrome. Lancet 1, 1158–60.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E., Philipp, M. & Ogilvie, B. M. (1981). Characterisation of surface antigens of Trichinella spiralis infective larvae. Parasite Immunology 3, 339–52.CrossRefGoogle ScholarPubMed
Philipp, M., Worms, M. J., Maizels, R. M. & Ogilvie, B. M. (1984). Rodent models of filariasis. Contemporary Topics in Immunobiology 12, 275–321.Google ScholarPubMed
Schroeder, L. L. (1985). An ELISA to detect antibody specific for surface antigens of parasitic nematodes. Journal of Immunological Methods 83, 135–40.CrossRefGoogle ScholarPubMed
Storey, N., Behnke, J. M. & Wakelin, D. (1984). Analysis of the immune response to Dipetalonema viteae infections in high and low responder mice. Parasitology 89, 66.Google Scholar
Storey, N., Wakelin, D. & Behnke, J. M. (1985). The genetic control of host responses to Dipetalonema viteae (Filarioidea) infections in mice. Parasite Immunology 7, 349–58.CrossRefGoogle ScholarPubMed
Thompson, J. P., Cbandall, R. B., Cbandall, C. A. & Neilson, J. T. (1979). Clearance of microfilariae of Dipetalonema viteae in CBA/N and CBA/H mice. Journal of Parasitology 65, 966–9.CrossRefGoogle ScholarPubMed
Worms, M. J., Tebby, R. J. & Tebby, A. (1961). Dipetalonema viteae, filarial parasite of the jird Meriones libycus. I. Maintenance in the laboratory. Journal of Parasitology 47, 963–70.CrossRefGoogle Scholar