Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-05T21:09:33.670Z Has data issue: false hasContentIssue false

Use of species-specific satellite DNAs as diagnostic probes in the identification of Steinernematidae and Heterorhabditidae entomopathogenic nematodes

Published online by Cambridge University Press:  06 April 2009

E. Grenier
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, BP2078, 06606 Antibes Cedex, France
E. Bonifassi
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, BP2078, 06606 Antibes Cedex, France
P. Abad
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, BP2078, 06606 Antibes Cedex, France
C. Laumond*
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, BP2078, 06606 Antibes Cedex, France
*
*Corresponding author. Tel: + 33 93 67 89 57 Fax: + 33 93 67 89 55. E-mail: [email protected].

Summary

Three satellite DNAs previously isolated from the entomopathogenic nematodes Steinernema carpocapsae, Heterorhabditis bacteriophora and Heterorhabditis indicus give hybridization signals only with the S. carpocapsae, H. bacteriophora and H. indicus populations tested, indicating that these satellite sequences are species-specific. Because of their reiteration and their variabilities, we have shown that these sequences are able to discriminate at the interspecific level between the Steinernema and Heterorhabditis species, but also at the intraspecific level between S. carpocapsae strains. Furthermore, in simple squashed nematode experiments, we are able to unambiguously identify S. carpocapsae, H. bacteriophora and H. indicus populations. This last procedure is effective even on a single infective juvenile, with the main advantage that it avoids time-consuming DNA extractions.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Akhurst, R. J. (1987). Use of gel electrophoresis in the taxonomy of the genus Heterorhabditis (Nematoda: Heterorhabditidae). Nematologica 33, 19.CrossRefGoogle Scholar
Bachmann, L., Schibel, J. M., Raab, M. & Sperlich, D. (1993). Satellite DNA as a taxonomic marker. Biochemical Systematics and Ecology 21, 311.CrossRefGoogle Scholar
Cabanillas, H. E., Poinar, G. O. & Raulston, J. R. (1994). Steinernema riobravis n.sp. (Rhabditida: Steinernematidae) from Texas. Fundamental and Applied Nematology 17, 123131.Google Scholar
Curran, J. (1990). Molecular techniques in taxonomy. In Entomopathogenic Nematodes in Biological Control (ed. Gaugler, R. & Kaya, H. K.), pp. 6374. CRC Press, Boca Raton, Ann Arbor, Boston.Google Scholar
Curran, J. & Webster, J. M. (1989). Genotypic analysis of Heterorhabditis isolates from North Carolina. Journal of Nematology 21, 140145.Google ScholarPubMed
Dix, I., Burnell, A. M., Griffin, C. T., Joyce, S. A., Nugent, M. J. & Downes, M. J. (1992). The identification of biological species in the genus Heterorhabditis (Nematoda: Heterorhabditidae) by cross-breeding second generation amphimictic adults. Parasitology 104, 509518.CrossRefGoogle Scholar
Doucet, M. M. A. De & Doucet, M. E. (1990). Steinernema ritteri n.sp. (Nematoda: Steinernematidae) with a key to the species of the genus. Nematologica 36, 257265.CrossRefGoogle Scholar
Feinberg, A. P. & Vogelstein, B. (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132, 613.CrossRefGoogle ScholarPubMed
Gardner, S. L., Stock, S. P. & Kaya, H. K. (1994). A new species of Heterorhabditis from the Hawaiian islands. Journal of Parasitology 80, 100106.CrossRefGoogle ScholarPubMed
Grenier, E., Laumond, C. & Abad, P. (1995). Characterization of a species-specific satellite DNA from the entomopathogenic nematode Steinernema carpocapsae. Molecular and Biochemical Parasitology 69, 93100.CrossRefGoogle ScholarPubMed
Joyce, S. A., Burnell, A. M. & Powers, T. O. (1994 a). Characterization of Heterorhabditis isolates by PCR amplification of segments of mtDNA and rDNA genes. Journal of Nematology 26, 260270.Google ScholarPubMed
Joyce, S. A., Griffin, C. T. & Burnell, A. M. (1994 b). The use of isoelectric focusing and polyacrylamide gel electrophoresis of soluble proteins in the taxonomy of the genus Heterorhabditis (Nematoda: Heterorhabditidae). Nematologica 40, 601612Google Scholar
Mracek, Z. (1994). Steinernema kraussei (Steiner, 1923) (Nematoda: Rhabditida: Steinernematidae): re-description of its topotype from Westphalia. Folia Parasitologica 41, 5964.Google Scholar
Mracek, Z., Arteaga, Hernandez E. & Boemare, N. E. (1994). Steinernema cubana sp.n. (Nematoda: Rhabditida: Steinernematidae) and the preliminary characterization of its associated bacterium. Journal of Invertebrate Pathology 64, 123129.CrossRefGoogle Scholar
Nguyen, K. B. & Smart, G. C. (1992). Steinernema neocurtillis n.sp. (Rhabditida, Steinernematidae) and a key to species of the genus Steinernema. Journal of Nematology 24, 463477.Google Scholar
Piotte, C., Castagnone-Sereno, P., Bongiovanni, M., Dalmasso, A. & Abad, P. (1995). Analysis of a satellite DNA from Meloidogyne hapla and its use as a diagnostic probe. Phytopathology 85, 458462.CrossRefGoogle Scholar
Poinar, G. O. (1990). Taxonomy and biology of Steinernematidae and Heterorhabditidae. In Entomopathogenic Nematodes in Biological Control (ed. Gaugler, R. & Kaya, H. K.), pp. 2361. CRC Press, Boca Raton, Ann Arbor, Boston.Google Scholar
Poinar, G. O., Karunakar, G. K. & David, H. (1992). Heterorhabditis indicus n.sp. (Rhabditida: Nematoda) from India: separation of Heterorhabditis spp. by infective juveniles. Fundamental and Applied Nematology 15, 467472.Google Scholar
Reid, A. P. & Hominick, W. M. (1993). Isolation and use of a species-specific clone for the identification of the rhabditid entomopathogenic nematode Steinernema feltiae (Filipjev, 1934). Fundamental and Applied Nematology 16, 115120.Google Scholar
Roman, J. & Figueroa, W. (1994). Steinernema puertoricensis n.sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Puerto Rico. Journal of Agriculture of the University of Puerto Rico 78, 167175.CrossRefGoogle Scholar
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn.Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.Google Scholar
Smits, P. H., Groenen, T. M. & De Raay, G. (1991). Characterization of Heterorhabditis isolates using DNA restriction fragment length polymorphism. Revue de Nématologie 14, 445453.Google Scholar
Southern, E. M. (1975). Detection of specific sequence among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98, 503517.CrossRefGoogle ScholarPubMed
Stock, S. P. (1993). A new species of the genus Heterorhabditis (Poinar, 1976) (Nematoda: Heterorhabditidae) parasitizing Graphognathus sp. larvae (Coleoptera: Curculionidae) from Argentina. Research and Reviews in Parasitology 53, 103107.Google Scholar
Tares, S., Lemontey, J. M., De Guiran, G. & Abad, P. (1994). Use of species specific satellite DNA from Bursaphelenchus xylophilus as a diagnostic probe. Phytopathology 84, 294298.CrossRefGoogle Scholar