Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-26T01:07:46.420Z Has data issue: false hasContentIssue false
  • English
  • Français

The ultrastructure and histochemistry of the cyst wall of Bucephalus haimeanus (Lacaze-Duthiers, 1854)

Published online by Cambridge University Press:  06 April 2009

Jacqueline C. Higgins ,
D. E. Wright  and
R. A. Matthews
Jacqueline C. Higgins
Affiliation:
School of Environmental Sciences, Plymouth Polytechnic, Drake Circus, Plymouth PL4 8AA, Devon
D. E. Wright
Affiliation:
School of Environmental Sciences, Plymouth Polytechnic, Drake Circus, Plymouth PL4 8AA, Devon
R. A. Matthews
Affiliation:
School of Environmental Sciences, Plymouth Polytechnic, Drake Circus, Plymouth PL4 8AA, Devon
Get access Rights & Permissions [Opens in a new window]

Extract

Metacercarial cysts of Bucephalus haimeanus occur throughout the liver of Pomatoschistus microps, greater concentrations being found around the gall bladder, blood vessels and periphery of the liver. The cyst wall is composed of three layers, an inner granular, middle vacuolated and outer nucleated layer. The inner layer consists of three zones, an inner zone of irregularly compacted, granular material; a middle densely compacted zone and an outer moderately compacted zone. Vacuoles of the middle layer vary in appearance and appear to be discharging their contents into the cyst cavity. The cells of the outer layer are flattened and elongated with elongate nuclei but no other distinct organelles. Occasionally at the outer edge of the cyst are electron-dense cells which are in the process of degeneration. Only small amounts of collagen and very few leucocytes are associated with the cysts.

The cyst wall is carbohydrate in nature containing some free aldehyde groups, galactogen and neutral mucosubstances. The inner layer appears to consist of mainly acidic mucopolysubstances and weakly acidic sulphomucins. Large amounts of protein, carbohydrate-protein complexes and DNA are also present within the cyst wall. Alkaline and acid phosphatases, ATPase, β-glucuronidase and N-acetyl-β-glucosaminidase were detected. The origin and function of the cyst wall is discussed.

Type
Research Article
Information
Parasitology , Volume 75 , Issue 2 , October 1977 , pp. 207 - 214
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adam, C. W. M. (1965). Neurohistochemistry. Amsterdam: Elsevier Publishing Company.Google Scholar
Asanji, M. F. & Williams, M. O. (1973). The structure and histochemistry of some trematode metacercarial cysts. Journal of Helminthology 47, 353–68.Google Scholar
Bogitsh, B. (1962). The chemical nature of metacercarial cysts. I. Histological and histo-chemical observations on the cysts of Posthodiplostomum minimum. Journal of Parasitology 48, 55–60.Google Scholar
Burgos, M. H., Vitale-Calpe, R. & TÉllezde IÑon, M. I. (1967). Studies on para-formaldehyde fixation for electron microscopy. I. Effect of concentration on ultrastructure. Journal de Microscopie 6, 457–68.Google Scholar
Chapman, J. A. & Hunter, G. W. III (1954). Studies on host-parasite reactions. VII. The pigment cells surrounding the metacercarial cysts of Cryptocotyle lingua in the Cunner Tautoglabrus adspersus (Walborn). Transactions of the American Microscopical Society 73, 28–36.Google Scholar
Drury, R. A. B. & Wallington, E. A. (1967). Carleton's Histological Technique. 4th ed.London: Oxford University Press.Google Scholar
Erasmus, D. A. (1962). Studies on the adult and metacercaria of Holostephanus luhei Szidat, 1936. Parasitology 52, 353–74.CrossRefGoogle Scholar
Gomori, G. (1952). Microscopic Histochemistry: Principles and Practice. Chicago: University of Chicago Press.Google Scholar
Hoffman, G. L. (1958). Experimental studies on the cercaria and metacercaria of a strigeoid trematode, Posthodiplostomum minimum. Experimental Parisitology 7, 23–50.Google Scholar
Hunter, G. W. III & Dalton, H. C. (1939). Studies on Clinostomum. V. The cyst of the yellow grub of fish (Clinostomum marginatum). Proceedings of the Helminthological Society of Washington 6, 73–6.Google Scholar
Hunter, G. W. III & Hamilton, J. M. (1941). Studies on host-parasite reactions to larval parasites. IV. The cyst of Uvulifer ambloplitis. Transactions of the American Microscopical Society 60, 498–507.Google Scholar
Hunter, G. W. III & Hunter, W. S. (1942). Studies on host-parasite reactions. V. The integumentary type of strigeid cyst. Transactions of the American Microscopical Society 61, 134–40.Google Scholar
Johnstone, J. (1905). Internal parasites and diseased conditions of fishes. Transactions of the Liverpool Biological Society 19, 278–300.Google Scholar
Lee, F. O. & Cheng, T. C. (1970). The histochemistry of Stellantchasmus falcatus Onji and Nisho, 1915 (Trematoda: Heterophyidae) metacercarial cyst in the mullet Mugil cephalus L. and histopathological alterations in the host. Journal of Fish Biology 2, 235–43.Google Scholar
Lumsden, R. (1968). Ultrastructure of the metacercarial cyst of Ascocotyle chandleri Lumsden, 1963 (Trematoda: Heterophyidae). Proceedings of the Helminthological Society of Washington 35, 212–19.Google Scholar
Matthews, R. A. (1973). The life-cycle of Bucephalus haimeanus. Lacaze-Duthiers, 1854 from Cardium edule L. Parasitology 67, 341–50.CrossRefGoogle ScholarPubMed
Matthews, R. A. (1974 a). The life-cycle of Bucephaloides gracilescens (Ruddphi, 1819) Hopkins, 1954 (Digenea: Gasterostomata). Parasitology 68, 1–12.CrossRefGoogle ScholarPubMed
Matthews, R. A. (1974 b). Metacercariae and disease in marine teleosts. 3rd International Congress of Parasitology, Munich, pp. 1723.Google Scholar
Mazia, D., Brewer, P. A. & Alfert, M. (1953). The cytochemical staining and measurement of proteins with mercuric bromphenol blue. Biological Bulletin 104, 57–67.CrossRefGoogle Scholar
McQueen, A., Mackenzie, K., Roberts, R. S. & Young, H. (1973). Studies on the skin of plaice, Pleuronectes platessa L. III. The effect of temperature on the inflammatory response to the metacercariae of Cryptocotyle lingua (Creplin, 1925). Journal of Fish Biology 5, 241–8.CrossRefGoogle Scholar
Mitchell, C. W. (1974). Ultrastructure of the metacercarial cyst of Posthodiplostomum minimum (MacCullum, 1921). Journal of Parasitology 60, 67–74.Google Scholar
Pande, B. P. & Shukla, R. P. (1972). Metacercarial cyst of Haplorchis pumilio, its development in experimental mammals and two other heterophyid infections of freshwater fishes and their zoonotic significance. Indian Journal of Animal Sciences 42, 871–978.Google Scholar
Papanicolaou, G. N. (1942). A new procedure for staining vaginal smears. Science 95, 438–9.Google Scholar
Papanicolaou, G. N. (1957). The cancer diagnostic potential of uterine exfoliative cytology. C. A. Bulletin of Cancer Progress 7, 125–35.Google Scholar
Pearse, A. G. E. (1968). Histochemistry: Theoretical and Applied, vol. I. London: H. and A. Churchill.Google Scholar
Peares, A. G. E. (1972). Histochemistry: Theoretical and Applied, vol. II. London: Churchill Livingstone.Google Scholar
Rai, P. (1969). Notes on the histochemistry of opisthorchiid, plagiochiid and metacercaria invasion of some Indian freshwater fishes. Indian Journal of Animal Sciences 39, 177–83.Google Scholar
Rees, P. G. (1955). The adult and diplostomulum stage (Diplostomum phoxini, Faust) of Diplostomum plematiodes Dubois and experimental demonstration of part of the life-cycle. Parasitology 45, 295–312.CrossRefGoogle ScholarPubMed
Reynolds, B. S. (1963). The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17, 208–12.CrossRefGoogle ScholarPubMed
Scott, J. E. & Dorling, J. (1965). Differential staining of acid glycosaminoglycans (muco-polysaccharides) by alcian blue in salt solutions. Histochemie 5, 221–33.Google Scholar
Smyth, J. D. (1956). Studies on tapeworm physiology. IX. A histochemical study of egg-shell formation in Schistocephalus solidus. (Pseudophillidean). Experimental Parasitology 5, 519–40.Google Scholar
Spicer, S. S. & Jarrels, M. H. (1961). Histochemical reaction of an aromatic diamine with acid groups and periodate engendered aldehydes in mucopolysaccharides. Journal of Histochemistry and Cytochemistry 9, 368–79.Google Scholar
Spurr, A. R. (1969). A low viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26, 31–43.Google Scholar
Stein, P. & Lumsden, R. (1971 a). The ultrastructure of developing metacercarial cysts of Ascocotyle leizhi Burton, 1956 (Heterophyidae). Proceedings of the Helminthological Society of Washington 38, 1–10.Google Scholar
Stein, P. & Lumsden, R. (1971 b). An ultrastructural and cytochemical study of metacercarial cyst development in Ascocotyle pachysistis Schroeder and Leigh, 1965. Journal of Parasitology 57, 1231–46.Google Scholar