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The ultrastructural localization of Echinococcus granulosus antigen 5

Published online by Cambridge University Press:  06 April 2009

M. K. Jones ,
L.-H. Zhang ,
G. R. Leggatt ,
D. J. Stenzel  and
D. P. McManus
M. K. Jones*
Affiliation:
Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia
L.-H. Zhang
Affiliation:
Tropical Health Program, Queensland Institute of Medical Research, 300 Herston Road, Herston, Qld 4029, Australia
G. R. Leggatt
Affiliation:
Tropical Health Program, Queensland Institute of Medical Research, 300 Herston Road, Herston, Qld 4029, Australia
D. J. Stenzel
Affiliation:
Analytical Electron Microscopy Facility, Queensland University of Technology, Brisbane, Qld 4001, Australia
D. P. McManus
Affiliation:
Tropical Health Program, Queensland Institute of Medical Research, 300 Herston Road, Herston, Qld 4029, Australia
*
* Corresponding author: Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Qld 4072, Australia. Tel: +61 7 3365 3249. Fax: +61 7 3365 2199. E-mail: [email protected].
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Summary

Murine monoclonal and polyclonal antisera, raised against the 38 kDa subunit of Echinococcus granulosus antigen 5, were used to investigate the tissue distribution of the antigen in hydatid cysts. Immunoreactivity was visualized by indirect immunofluorescence on whole protoscoleces, and ultrastructural immunocytochemistry utilizing colloidal gold-based labelling procedures on unsectioned and cryosectioned brood capsules and protoscoleces. In protoscoleces, the 38 kDa subunit of antigen 5 was localized at the interface of parenchymal cells and associated extracellular matrices, as well as along the interface of the tegumentary syncytium in the somal region and its basal matrix. Cytoplasmic labelling of parenchymal cells was rare; when observed, it was associated with vesicles and membranes in cytoplasmic extensions of parenchymal cells. In brood capsules, the antigen was associated with the external face of the plasma of degenerating parenchymal cells. The 38 kDa subunit occurs along the extracellular face of cell membranes, suggesting that antigen 5 is either a component of the membranes or associated extracellular matrices.

Keywords

Echinococcus granulosushydatid cystimmunocytochemistryantigen 5
Type
Research Article
Information
Parasitology , Volume 113 , Issue 3 , September 1996 , pp. 213 - 222
Copyright
Copyright © Cambridge University Press 1996

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