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The ultrastructural development of the oocyst wall of Eimeria maxima

Published online by Cambridge University Press:  06 April 2009

R. M. Pittilo  and
S. J. Ball
R. M. Pittilo
Affiliation:
Department of Biology, North East London Polytechnic, Romford Road, London E15 4LZ
S. J. Ball
Affiliation:
Department of Biology, North East London Polytechnic, Romford Road, London E15 4LZ
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Summary

Oocyst wall formation in Eimeria maxima was studied during the macrogamete stage in intestinal cells of the chick and in unsporulated oocysts isolated from faeces. The outer of the 2 membranes bounding the mature macrogamete separated from the surface but remained as a veil surrounding the developing oocyst throughout the whole intracellular process. Wall-forming bodies Type I were initially applied to the limiting membrane of the zygote cytoplasm; a layer of material similar to their contents was then formed around the zygote. As this occurred a new double membrane was formed surrounding the oocyst cytoplasm. The outer wall layer was initially homogenous in appearance but later developed into 2 zones, an outer amorphous region and an inner osmiophilic region. The inner layer of the oocyst wall was formed from the contents of the wallforming bodies Type II which dispersed between the outer wall and the limiting membranes of the oocyst cytoplasm. There was evidence of an additional membrane formed external to the outer wall. The outer membranes were not present around the wall of oocysts passed in the faeces of chicks, but the same wall zonation was evident, although the inner osmiophilic zone of the outer wall layer was markedly thinner in comparison with the same zone seen in the tissues.

Type
Research Article
Information
Parasitology , Volume 81 , Issue 1 , August 1980 , pp. 115 - 122
Copyright
Copyright © Cambridge University Press 1980

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References

REFERENCES

Birch-Andersen, A., Ferguson, D. J. P., & Pontefract, R. D., (1976). A technique for obtaining thin sections of coccidian oocysts. Acta pathologica et microbiologica Scandinavica, Section B 84, 235–9.Google Scholar
Chobotar, B., Sénaud, J., Ernst, J. V., & Scholtyseck, E., (1975). The ultrastructure of macrogametes of Eimeria ferrisi Levine and Ivens 1965 in Mus musculus. Zeitschrift für Parasitenkunde 48, 111–21.CrossRefGoogle ScholarPubMed
Doens-Juteau, O., & Sénaud, J., (1974). Étude ultrastructurale de la paroi du macrogamète et de l'oocyste chez Eimeria tenella (Railliet et Lucet, 1891). Coccidie Eimeriidae, parasite de l'intestin des poulets. Protistologica 10, 261–70.Google Scholar
Dubremetz, J. F., & Yvore, P., (1971). Elaboration de la coque oocystique chez la Coccidie Eimeria necatrix Johnson 1930 (Sporozoaires, Coccidiomorphes). Étude au microscope électronique. Comptes rendus des séances de la Société de biologie. Paris 165, 862–6.Google Scholar
Ferguson, D. J. P., Birch-andersen, A., Hutchison, W. M., & Sum, J. Chr., (1977). Ultrastructural studies on the endogenous development of Eimeria brunetti. IV. Formation and structure of the oocyst wall. Acta pathologica et microbiologica Scandinavica, Section B 85, 201–11.Google Scholar
Lee, D. L., & Millard, B. J., (1971). The structure and development of the macrogamete and oocyst of Eimeria acervulina. Parasitology 62, 31–4.CrossRefGoogle ScholarPubMed
McLaren, D. J., (1969). Observations on the fine structural changes associated with schizogony and gametogony in Eimeria tenella. Parasitology 59, 563–74.Google Scholar
Mehlhorn, H., (1972). Elektronenmikroskopische Untersuchungen an Entwicklungsstadien von Eimeria maxima (Sporozoa, Coccidia). I. Die Feinstruktur der Makrogameten. Zeitschriftfür Parasitenkunde 39, 161–82.Google Scholar
Michael, E., (1978). The formation and final structure of the oocyst wall of Eimeria acervulina: a transmission and scanning electron microscope study. Zeitschrift für Parasitenkunde 57, 221–8.Google Scholar
Pittilo, R. M., & Ball, S. J., (1979). The fine structure of the developing macrogamete of Eimeria maxima. Parasitology 79, 259–65.Google Scholar
Reynolds, E., 8. (1963). The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17, 208–12.Google Scholar
Roberts, W. L., Speer, C. A., & Hammond, D. M., (1970). Electron and light microscope studies of the oocyst walls, sporocysts, and excysting sporozoites of Eimeria callospermophili and Eimeria larimerensis. Journal of Parasitology 56, 918–26.Google Scholar
Scholtyseck, E., (1973). Ultrastructure. In The Coccidia (ed. Hammond, D. M. and Long, P. L.), pp. 81–144. Baltimore: University Park Press.Google Scholar
Scholtyseck, E., Mehelhorn, H., & Hammond, D. M., (1971). Fine structure of macrogametes and oocysts of coccidia and related organisms. Zeitschrift für Parasitenkunde 37, 1–43.Google Scholar
Scholtyseck, E., Rommel, A., & Heller, G., (1969). Licht- und elektronenmikroskopische Unterauchungen zur Bildung der Oocystenhülle bei Eimerien (Eimeria perforans, E. stiedae and E. tenlla). Zeitschrift für Parasitenkunde 31, 289–98.Google Scholar
Speer, C. A., & Duszynski, D. W., (1975). Fine structure of the oocyst walls of Isospora serini and Isospora canaria and excystation of Isospora serini from the canary, Serinus canarius L. Journal of Protozoology 22, 476–81.Google Scholar
Varghese, T., (1975). The fine structure of the endogenous stages of Eimeria labbeana 2. Mature macrogamonts and young oocysts. Zeitschrift für Parasitenkunde 46, 43–51.Google Scholar
Wheat, B. E., Jensen, J. B., Ernst, J. V., & Chobotar, B., (1976). Ultrastructure of macrogametogenesis of Eimeria mivati. Zeitschrift für Parasitenkunde 50, 125–36.Google Scholar