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Ultrastructural and molecular characterization of Bacillidium vesiculoformis n. sp. (Microspora: Mrazekiidae) in the freshwater oligochaete Nais simplex (Oligochaeta: Naididae)

Published online by Cambridge University Press:  13 December 2004

D. J. MORRIS ,
R. S. TERRY ,
K. B. FERGUSON ,
J. E. SMITH  and
A. ADAMS
D. J. MORRIS
Affiliation:
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
R. S. TERRY
Affiliation:
School of Biology, University of Leeds, Leeds LS2 9JT, UK
K. B. FERGUSON
Affiliation:
Dollar Academy, Dollar, Clackmannanshire FK14 7DU, UK
J. E. SMITH
Affiliation:
School of Biology, University of Leeds, Leeds LS2 9JT, UK
A. ADAMS
Affiliation:
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
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Abstract

The development of a new species, Bacillidium vesiculoformis n. sp. (Microspora, Mrazekiidae), is described from the freshwater oligochaete Nais simplex (Oligochaeta, Naididae). Initial stages of parasite development consist of a monokaryotic merogony within a haemocyte of the intestinal blood sinus. The resulting hypertrophied haemocyte is attached to the chloragocytes of the sinus by fine cytoplasmic extensions with the sinus around the cell becoming greatly enlarged. The meronts within the haemocyte form diplokaryotic sporonts that undergo sporogenesis directly within the cytoplasm of the host cell. The infected cell becomes packed with spores and developmental stages, causing it dramatically to increase in size, eventually rupturing the oligochaete and cell. Sporogony appears to be disporoblastic. Released spores were observed to have an adhesive quality. Transmission studies conducted with mature spores failed to transmit the parasite horizontally although vertical transmission was observed. Phylogenetic analysis of the parasite demonstrated that B. vesiculoformis clustered with microsporidian parasites of bryozoa and two other microsporidians, Janacekia debaiseuxi and an unidentified Bacillidium sp.

Keywords

Bryozoalife-cyclemanubriumPseudonosema
Type
Research Article
Information
Parasitology , Volume 130 , Issue 1 , January 2005 , pp. 31 - 40
Copyright
© 2004 Cambridge University Press

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References

REFERENCES

BAKER, M. D., VOSSBRINCK, C. R., DIDIER, E. S., MADDOX, J. V. & SHADDUCK, J. A. ( 1995). Small subunit ribosomal DNA phylogeny of various microsporidia with emphasis on AIDS related forms. Journal of Eukaryotic Microbiology 42, 564–570.CrossRefGoogle Scholar
CANNING, E. U., REFARDT, D., VOSSBRINCK, C. R., OKAMURA, B. & CURRY, A. ( 2002). New diplokaryotic microsporidia (Phylum Microsporidia) from freshwater bryozoans (Bryozoa, Phylactolaemata). European Journal of Protistology 38, 247–265.CrossRefGoogle Scholar
GATEHOUSE, H. S. & MALONE, L. A. ( 1998). The ribosomal RNA gene region of Nosema apis (Microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size. Journal of Invertebrate Pathology 71, 97–105.CrossRefGoogle Scholar
GOTZ, P. ( 1981). Homology of the manubrium of Mrazekia brevicauda and the polar filament of other Microsporidia. Zeitschrift für Parasitenkunde 64, 321–333.Google Scholar
HALL, T. ( 2001). Bioedit: Biological sequence alignment editor for Windows 95/98/NT Edition 5.0.9. http://www.mbio.ncsu.edu/BioEdit/bioedit.html.
JANDA, V. ( 1928). Über Microorganismen aus der Leibshöhle von Criodrilus lacuum Hoffm. und eigenartige Neubildungen in der Körperwand dieses Tieres. Archive für Protistenkunde 63, 84–93.Google Scholar
JIROVEC, O. ( 1936). Zur kenntnis von Oligochäten parasitierenden Microspodien aus der Familie Mrázekidae. Archive für Protistenkunde 87, 314–344.Google Scholar
KATHMAN, R. D. & BRINKHURST, R. O. ( 1999). Guide to the Freshwater Oligochaetes of North America. Aquatic Resources Center, College Grove, Tennessee, USA.
LARSSON, J. I. R. ( 1989). The light and electron microscopic cytology of Bacillidium filiferum sp. nov. (Microspora, Bacillidiidae). Archive für Protistenkunde 137, 346–355.Google Scholar
LARSSON, J. I. R. ( 1994). Characteristics of the genus Bacillidium Janda, 1928 (Microspora, Mrazekiidae)- reinvestigation of the type species B. criodrili and improved diagnosis of the genus. European Journal of Protistology 30, 85–96.Google Scholar
LARSSON, J. I. R. & GOTZ, P. ( 1996). Ultrastructural evidence for the systematic position of Jirovecia brevicauda (Leger & Hesse, 1916) Weisser, 1985 (Microspora, Mrazekiidae). European Journal of Protistology 32, 366–371.CrossRefGoogle Scholar
MORRIS, D. J. & ADAMS, A. ( 2002). Development of Schroedera plumatellae gen.n., sp. n. (Microsporidia) in Plumatella fungosa (Bryozoa: Phylactolaemata). Acta Protozoologica 41, 383–396.Google Scholar
MORRIS, D. J., MORRIS, D. C. & ADAMS, A. ( 2002). Development and release of a malacosporean (Myxozoa) from Plumatella repens (Bryozoa: Phylactolaemata). Folia Parasitologica 49, 25–34.CrossRefGoogle Scholar
NILSEN, F. ( 1999). Small subunit rDNA phylogeny of Bacillidium sp. (Microspora, Mrazekiidae) infecting oligochaets. Parasitology 118, 553–558.Google Scholar
SWOFFORD, D. L. ( 2002). PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods), v. 4.0b10. Sinauer Associates, Sunderland, Mass.
TERRY, R. S., MacNEIL, C., DICK, J. T. A., SMITH, J. E. & DUNN, A. M. ( 2003). Resolution of a taxonomic condundrum: an ultrastructural and molecular description of the life cycle of Pleistophora mulleri (Pfeiffer 1895; Georgevitch 1929). Journal of Eukaryotic Microbiology 50, 266–273.CrossRefGoogle Scholar
VOSSBRINCK, C. R., BAKER, M. D., DIDIER, E. S., DEBRUNNER-VOSSBRINCK, B. A. & SHADDUCK, J. A. ( 1993). Ribosomal DNA sequences of Encephalitozoon hellem and Encephalitozoon cuniculi: species identification and phylogenetic construction. Journal of Eukaryotic Microbiology 40, 354–362.CrossRefGoogle Scholar
WEISS, L. M., ZHU, X., CALI, A., TANOWITZ, H. B. & WITTNER, M. ( 1994). Utility of microsporidian rRNA in diagnosis and phylogeny: a review. Folia Parasitologica 41, 81–90.Google Scholar
WOOD, T. S. ( 1989) Ectoproct bryozoans of Ohio. Ohio Biological Survey Bulletin 8, 1–70.Google Scholar