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Ultrastructural and molecular characterization of Bacillidium vesiculoformis n. sp. (Microspora: Mrazekiidae) in the freshwater oligochaete Nais simplex (Oligochaeta: Naididae)

Published online by Cambridge University Press:  13 December 2004

D. J. MORRIS ,
R. S. TERRY ,
K. B. FERGUSON ,
J. E. SMITH  and
A. ADAMS
D. J. MORRIS
Affiliation:
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
R. S. TERRY
Affiliation:
School of Biology, University of Leeds, Leeds LS2 9JT, UK
K. B. FERGUSON
Affiliation:
Dollar Academy, Dollar, Clackmannanshire FK14 7DU, UK
J. E. SMITH
Affiliation:
School of Biology, University of Leeds, Leeds LS2 9JT, UK
A. ADAMS
Affiliation:
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
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Abstract

The development of a new species, Bacillidium vesiculoformis n. sp. (Microspora, Mrazekiidae), is described from the freshwater oligochaete Nais simplex (Oligochaeta, Naididae). Initial stages of parasite development consist of a monokaryotic merogony within a haemocyte of the intestinal blood sinus. The resulting hypertrophied haemocyte is attached to the chloragocytes of the sinus by fine cytoplasmic extensions with the sinus around the cell becoming greatly enlarged. The meronts within the haemocyte form diplokaryotic sporonts that undergo sporogenesis directly within the cytoplasm of the host cell. The infected cell becomes packed with spores and developmental stages, causing it dramatically to increase in size, eventually rupturing the oligochaete and cell. Sporogony appears to be disporoblastic. Released spores were observed to have an adhesive quality. Transmission studies conducted with mature spores failed to transmit the parasite horizontally although vertical transmission was observed. Phylogenetic analysis of the parasite demonstrated that B. vesiculoformis clustered with microsporidian parasites of bryozoa and two other microsporidians, Janacekia debaiseuxi and an unidentified Bacillidium sp.

Keywords

Bryozoalife-cyclemanubriumPseudonosema
Type
Research Article
Information
Parasitology , Volume 130 , Issue 1 , January 2005 , pp. 31 - 40
Copyright
© 2004 Cambridge University Press

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