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Ultrastructural and cytochemical studies on the sensory organelles and nervous system of Dipetalonema viteae (Nematoda: Filarioidea)

Published online by Cambridge University Press:  06 April 2009

Diane J. McLaren
Diane J. McLaren
Affiliation:
National Institute for Medical Research, Mill Hill, London NW7 1AA
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Extract

The sensory organelles and some components of the nervous system of the adult filarial worm Dipetalonema viteae have been studied by means of electron microscopy. The sensory organelles contain modified cilia innervated by nerve axons. The cilia lie within cuticle-lined channels. The amphids each contain nine such cilia, but the phasmids and the papillae contain only one cilium. The amphidial and phasmidial cilia are exposed to the external environment and it is suggested that I they function as chemoreceptors. The cilia within the cephalic and caudal papillae are modified so as to produce a greater area of sensitivity. The ciliary channels in the cephalic papillae do not open to the exterior and these papillae therefore probably function as mechanoreceptors. The caudal papillae of the male worm are structurally similar to the cephalic papillae, except that the ciliary channels are open to the external environment. It is suggested that these papillae function both as mechanoreceptors and chemoreceptors. The terminal pair of caudal papillae differ from all the other papillae. The ciliary channels of all the sensory organelles are surrounded by supporting cells, but multivesicular cells are only associated K with the amphids, phasmids and cephalic papillae. The nerve-ring encircles the muscular region of the oesophagus; it is composed entirely of nerve axons. The nerve axons contain two kinds of vesicle. The nervering is surrounded by an ensheathing cell. The major nerve bundles are carried by the hypodermal cords, but they are not embedded in the hypodermal cytoplasm. The nerve axons at the surface of the cord form synapses with the innervation processes of the somatic muscle cells. Electron cytochemical techniques have been used to investigate the distribution of cholinesterases in the structures described. Acetylcholinesterase has been identified in the amphids, phasmids, cephalic papillae and nerve-ring; it is suggested that acetylcholine functions as a chemical transmitter at these sites. Pseudocholinesterase has been identified in the amphids, phasmids and cephalic papillae, but not the nerve-ring; it is present in considerably smaller quantities than acetylcholinesterase.

Type
Research Article
Information
Parasitology , Volume 65 , Issue 3 , December 1972 , pp. 507 - 524
Copyright
Copyright © Cambridge University Press 1972

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References

Bayliss, B. & Todrick, A. (1956). The use of a selective acetylcholinesterase inhibitor in the estimation of pseudocholinesterase activity in rat brain. Biochemical Journal 62, 62–7.Google Scholar
Bird, A. F. (1966). Esterases in the genus Meloidogyne. Nematologica 12, 359–61.Google Scholar
Eccles, J. C., (1964). Structural features of chemically transmitting synapses. In The Physiology of Synapses, pp. 11–26. Berlin, Heidelberg, New York: Springer-Verlag.Google Scholar
Gibbons, I. R. & Grimstone, A. V. (1960). On flagellar structure in certain flagellates. Journal of Biophysical and Biochemical Cytology 7, 697–715.CrossRefGoogle ScholarPubMed
de Habven, E. (1967). Methods in electron microscope cytology. In Methods in Cancer Research, vol. 1 (ed. Busch, H..), pp. 3–44. London and New York: Academic Press.Google Scholar
Hope, W. D. (1965). A taxonomic review of the genus Thoracostoma Marion, 1870 (Nemata: Leptosomatidae), and a study of the histologic morphology of Thoracostoma califomicum Steiner and Albin 1933. Ph.D. Thesis, University of California, Davis.Google Scholar
Horridge, G. A. (1965). Non-motile sensory cilia and neuromuscular junctions in a ctenophore independent effector organ. Proceedings of the Royal Society B 162, 333–50.Google Scholar
Kozek, W. J. (1968). Unusual cilia in the microfilaria of Dirofllaria immitis. Journal of Parasitology 54, 838–44.Google Scholar
Lee, D. L. (1962). The distribution of esterase enzymes in Ascaris lumbricoides. Parasitology 52, 241–60.CrossRefGoogle Scholar
Lee, D. L. (1964). Esterase enzymes in two free-living nematodes. Proceedings of the Helminthological Society of Washington 31, 285–8.Google Scholar
Lee, D. L. (1966). An electron microscope study of the body wall of the third stage larva of Nippostrongylus brasiliensis. Parasitology 56, 127–35.Google Scholar
Lewis, P. R. & Shute, C. C. D. (1966). The distribution of cholinesterase in cholinergic neurones demonstrated with the electron microscope. Journal of Cell Science 1, 381–390.Google Scholar
López Abella, D., Jiménez-Millán, F. & Garcia-Hidalgo, F. (1967). Electron microscope studies of some cephalic structures of Xiphinema americanum. Nematologica 13, 283–6.Google Scholar
McLaren, D. J. (1969). Ciliary structures in the microfilaria of Loa loa. Transactions of the Royal Society of Tropical Medicine and Hygiene 63, 290–1.Google Scholar
McLaren, D. J. (1970). Preliminary observations on the sensory structures in adult filariae. Transactions of the Royal Society of Tropical Medicine and Hygiene 64, 191–2.Google Scholar
McLaren, D. J. (1972). Ultrastructural studies on microfilariae (Nematoda: Filarioidea). Parasitology 65, 317.Google Scholar
Millonig, G. (1961). Advantages of a phosphate buffer for osmium tetroxide solutions in fixation. Journal of Applied Physiology 32, 1637.Google Scholar
Okano, M., Weber, A. F. & Fromes, S. P. (1967). Electron microscope studies on the distal border of the canine olfactory epithelium. Journal of Ultrastructure Research 17, 487–500.Google Scholar
Poinar, G. O. & Leutenegger, R. (1968). Anatomy of the infective and normal third stage juveniles of Neoaplectana carpocapsae Weiser (Steinernematidea: Nematoda). Journal of Parasitology 54, 340–50.Google Scholar
Porter, K. R. & Bonneville, M. A., (1964). In An Introduction to the Pine Structure of Cells and Tissues, 2nd ed.London: Henry Kimpton.Google Scholar
Raski, D. J., Jones, N. O. & Roggen, D. R. (1969). On the morphology and ultrastructure of the oesophageal region of Trichodorus allius. Proceedings of the Helminthological Society of Washington 36, 106–18.Google Scholar
Ringo, D. L. (1967). Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas. Journal of Cell Biology 33, 543–71.Google Scholar
Roggen, D. R, Raski, D. J. & Jones, N. O. (1966). Cilia in nematode sensory organs. Science, New York 152, 515.Google Scholar
Roggen, D. R, Raski, D. J. & Jones, N. O. (1967). Further electron microscope observations of Xiphinema index. Nematologica 13, 1–16.Google Scholar
Rohde, R. A. (1960). Acetylcholinesterase in plant parasitic nematodes and an anticholinesterase from asparagus. Proceedings of the Helminthological Society of Washington 27, 121–3.Google Scholar
Rosenbluth, J. (1965). Ultrastructure of somatic muscle cells in Ascaris lumbricoides. II. Intermuscular junctions, neuromuscular junctions and glycogen stores. Journal of Cell Biology 26, 579–91.Google Scholar
Ross, M. M. R. (1967). Modified cilia in sensory organs of juvenile stages of a parasitic nematode. Science, New York 156, 1494–1495.Google Scholar
Slifer, E. H. (1961). The fine structure of insect sense organs. International Review of Cytology 11, 125–59.Google Scholar
Slifer, E. H. & Sekhon, S. S. (1964). The dendrites of the thin-walled olfactory pegs of the grasshopper Orthoptera, Acrididae. Journal of Morphology 114, 393–409.Google Scholar
Slifer, E. H, Sekhon, S. S. & Lees, A. D. (1964). The sense organs on the antennal flagellum of aphids (Homoptera) with special reference to the plate organs. Quarterly Journal of Microscopical Science 105, 21–9.Google Scholar
Thurm, U. (1964). Mechanoreceptors in the cuticle of the honey bee: fine structure and stimulus mechanism. Sceince, New York 145, 1063–5.Google Scholar
Venable, J. H. & Coggleshall, R. (1965). A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25, 407–8.Google Scholar