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A trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture, Japan

Published online by Cambridge University Press:  19 February 2007

O. M. M. THEKISOE ,
T. HONDA ,
H. FUJITA ,
B. BATTSETSEG ,
T. HATTA ,
K. FUJISAKI ,
C. SUGIMOTO  and
N. INOUE
O. M. M. THEKISOE
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
T. HONDA
Affiliation:
Kagoshima Prefectural Center for Environmental Research and Public Health, Kagoshima, 892-0853, Japan
H. FUJITA
Affiliation:
Ohara Research Laboratory, Ohara General Hospital, Fukushima, 960-0915, Japan
B. BATTSETSEG
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
T. HATTA
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
K. FUJISAKI
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
C. SUGIMOTO
Affiliation:
Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan
N. INOUE*
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan
*
*Corresponding author: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Tel: +81 155 49 5647. Fax: +81 155 49 5643. E-mail: [email protected]
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Summary

Common arthropod vectors for trypanosomes are flies, fleas and bugs. This study reports on an unknown trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks, hereby, referred to as Trypanosoma KG1 isolate. The parasite has been successfully cultured in vitro with L929 or HEK 293T cell line as feeder cells. This trypanosome cannot survive in vitro without feeder cells. Following experimental infections of ticks, the trypomastigote-like and the epimastigote-like forms of this trypanosome could be detected by Giemsa-stained smears of the midgut and salivary glands of Ornithodoros moubata ticks which were made to feed on a culturing medium containing Trypanosoma KG1 isolate through an artificial membrane. Trypanosoma KG1 isolate could also be detected from Giemsa-stained smears of the haemolymph up to 30 days post-inoculation into the O. moubata haemocoel. Trypanosoma KG1 isolate cannot be propagated in laboratory animals including mice, rats, rabbits and sheep. A phylogenetic tree constructed with the 18S rRNA gene indicates that Trypanosoma KG1 is a member of the stercorarian trypanosomes.

Keywords

Trypanosoma KG1 isolateHaemaphysalis hystricisOrnithodoros moubata
Type
Research Article
Information
Parasitology , Volume 134 , Issue 7 , July 2007 , pp. 967 - 974
Copyright
Copyright © Cambridge University Press 2007

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