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Trypanosomatid biodiversity in Costa Rica: genotyping of parasites from Heteroptera using the spliced leader RNA gene

Published online by Cambridge University Press:  05 October 2004

S. J. WESTENBERGER ,
N. R. STURM ,
D. YANEGA ,
S. A. PODLIPAEV ,
R. ZELEDÓN ,
D. A. CAMPBELL  and
D. A. MASLOV
S. J. WESTENBERGER
Affiliation:
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California – Los Angeles, Los Angeles, CA 90095-1487, USA
N. R. STURM
Affiliation:
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California – Los Angeles, Los Angeles, CA 90095-1487, USA
D. YANEGA
Affiliation:
Department of Entomology and Entomology Research Museum, University of California – Riverside, Riverside, CA 92521, USA Zoological Institute, Russian Academy of Sciences, 199034 St. Petersburg, Russia
S. A. PODLIPAEV
Affiliation:
Zoological Institute, Russian Academy of Sciences, 199034 St. Petersburg, Russia
R. ZELEDÓN
Affiliation:
School of Veterinary Medicine, National University, Heredia, Costa Rica
D. A. CAMPBELL
Affiliation:
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California – Los Angeles, Los Angeles, CA 90095-1487, USA
D. A. MASLOV
Affiliation:
Department of Biology, University of California – Riverside, Riverside, CA 92521, USA
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Abstract

The biodiversity of insect trypanosomes is largely unknown, resulting in significant gaps in the understanding of pathogen evolution. A culture-independent preliminary survey of trypanosomatid fauna was conducted for the parasites of Heteroptera (Hemiptera) from several localities in Costa Rica. Trypanosomatid infections were detected by light microscopy of smeared gut contents. Out of 257 insects representing 6 families, infections were found in 62 cases; cultures were obtained for 29 new isolates. Gut material from infected hosts was preserved in the field using an SDS–EDTA buffer solution for subsequent DNA extraction in the laboratory. PCR amplification of the trypanosomatid-specific spliced leader (SL) RNA gene repeats was successful for 60 field samples. Eighteen distinct SL RNA typing units were identified in a set of 28 samples analysed in detail. Cluster analysis indicated that these typing units were unique and thus could represent new species and, in some cases, new genera. This study reveals only a minor fraction of the trypanosomatid biodiversity, which is anticipated to be high.

Keywords

Trypanosomatidaebiodiversitymini-exonSL RNAgenotyping
Type
Research Article
Information
Parasitology , Volume 129 , Issue 5 , November 2004 , pp. 537 - 547
Copyright
© 2004 Cambridge University Press

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