Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-30T23:22:21.020Z Has data issue: false hasContentIssue false
  • English
  • Français

Trypanosoma (Schizotrypanum) dionisii of Pipistrellus pipistrellus (Chiroptera): intra- and extracellular development in vitro

Published online by Cambridge University Press:  06 April 2009

J. R. Baker ,
Sheila M. Green ,
Lisbeth A. Chaloner  and
Maria Gaborak
J. R. Baker
Affiliation:
M.R.G. Biochemical Parasitology Unit, Molteno Institute, Downing Street, Cambridge, CB2 3EE, England
Sheila M. Green
Affiliation:
M.R.G. Biochemical Parasitology Unit, Molteno Institute, Downing Street, Cambridge, CB2 3EE, England
Lisbeth A. Chaloner
Affiliation:
M.R.G. Biochemical Parasitology Unit, Molteno Institute, Downing Street, Cambridge, CB2 3EE, England
Maria Gaborak
Affiliation:
M.R.G. Biochemical Parasitology Unit, Molteno Institute, Downing Street, Cambridge, CB2 3EE, England
Get access Rights & Permissions [Opens in a new window]

Extract

A trypanosome identified as Trypanosoma (Schizotrypanum) dionisii Bettencourt & França, 1905, has been isolated from Pipistrellus pipistrellus (Chiroptera) in England. At least five out of eight P. pipistrelhis were infected.2. In the blood of P. pipistrelhis, the parasite closely resembled T. (S.) cruzi. When grown in vitro in monophasic or diphasic media at 28°C, epimastigotes and trypomastigotes developed. The latter were of two types — very long, thin forms and less numerous shorter individuals.3. The trypanosomes multiplied as amastigotes within HeLa and mouse L cells in vitro. After 6–9 days in HeLa cells at 37°C, they transformed into small trypomastigotes and emerged from the cells. Higher infection rates (up to about 4%) were obtained in cell cultures inoculated with flagellates from older monophasic cultures, which contained more of the long, slender trypomastigotes.4. Trypanosomes also entered (or were phagocytosed by) up to 60% or more of mouse peritoneal macrophages in vitro. Multiplication occurred by binary and multiple fission of amastigotes in at least some of the parasitized macrophages and transformation into trypomastigotes was seen after 7 or more days at 37°C. Development in macrophages was less synchronous than in HeLa cells.

Type
Research Article
Information
Parasitology , Volume 65 , Issue 2 , October 1972 , pp. 251 - 263
Copyright
Copyright © Cambridge University Press 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akiyama, H. J., & Haight, R. D., (1971). Interaction of Leishmania donovani and hamster peritoneal macrophages. American Journal of Tropical Medicine and Hygiene 20, 539–45.CrossRefGoogle ScholarPubMed
Bafort, J. M., Molyneux, D., & Racey, P. A., (1970). The sub-genus Schizotrypanum in Britain. Transactions of the Royal Society of Tropical Medicine and Hygiene 64, 472.CrossRefGoogle ScholarPubMed
Baker, J. R., Chaloner, L. A., & Green, S. M., (1971). Intracellular development in vitro of Trypanosoma dionisii of bats. Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 427.Google ScholarPubMed
Baker, J. R., Green, S. M., Chaloner, L. A., & Gaborak, M., (1972). Intracellular growth in vitro of Trypanosoma (Schizotrypanum) dionisii of bats and preliminary work on cellmediated immunity using this system. Transactions of the Royal Society of Tropical Medicine and Hygiene 66, 340–1.CrossRefGoogle ScholarPubMed
Baker, J. R., & Thompson, G. B., (1971). Two species of Trypanosoma from British Chiroptera (Bats). Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 427.Google Scholar
Barretto, M. P., (1970). Estudos sôbre reservatórios e vectores silvestres do Trypanosoma cruzi. XLIII. Sôbre a validade das espécies americanos de flagelados incluídos no subgênero Schizotrypanum Chagas, 1909 do gênero Trypanosoma Gruby, 1843. Revista do Instituto de medicina tropical de São Paulo 12, 272–8.Google Scholar
Battaglia, M., (1904). Alcune ricerche sopra due tripanosomi (Trypanosoma vespertilionis, Trypanosoma lewisi). Annali di medicina navale e coloniale 10 (2), 517–23.Google Scholar
Bettencourt, A., & França, C., (1905). Sur un trypanosome de la chauve-souris. Compte rendu des séances de la Société de biologie 59, 305–7.Google Scholar
Bettencourt, A., & França, C., (1906). Sur un trypanosome de la chauve-souris. Archivos do R. Instituto bacteriológico Câmara Pestana 1, 187–94.Google Scholar
Brack, C., (1968). Elektronmikroskopische Untersuchungen zum Lebenszyklus von Trypano soma cruzi. Acta tropica 25, 289–356.Google Scholar
Bray, R. S., & Garnham, P. C. C., (1962). The Giemsa colophonium method for staining protozoa in tissue sections. Indian Journal of Malariology 16, 153–5.Google ScholarPubMed
Chatton, E., & Courrier, R., (1921 a). Sur un trypanosome de la chauve-souris Vesperugo pipistrellus, à formes crithidiennes intratissulaire et cystigènes. Hypothèse relative à l'étiologie du goître endémique. Compte rendu hebdomadaire des seances de l'Académie des sciences, Paris 172, 1254–7.Google Scholar
Chatton, E., & Courrier, R., (1921 b). Un Schizotrypanum chez les chauve-souris (Vesperugo pipistrellus) en Basse-Alsace. Schizotrypanose et goître endémique. Compte rendu des stances de la Société de biologie 84, 943–6.Google Scholar
Coles, A. C., (1914). Blood parasites found in mammals, birds and fishes in England. Parasitology 7, 17–61.CrossRefGoogle Scholar
Dias, E., (1936). Revisão geral dos hemoflagellados de Chiropteros. 9 Reunión de la Sociedad Argentina de Patologia Regional 1, 10–88.Google Scholar
Dionisi, A., (1899). La malaria di alcune specie di pipistrelli. Atti della Societa per gli Studi delta Malaria 1, 133–73.Google Scholar
Franchini, G., (1921). Trypanosome de la chauve-souris en Italie. Formes viscérales et stades de développement chez un acarien gamaride, le Leiognathus laverani, n.sp. Bulletin de la Société de pathologie exotique 14, 542–6.Google Scholar
Goedbloed, E., Cremers-Hoyer, L., & Perie, N. M., (1964). Blood parasites of bats in the Netherlands. Annals of Tropical Medicine and Parasitology 58, 257–60.CrossRefGoogle ScholarPubMed
Hoare, C. A., (1966). The classification of mammalian trypanosomes. Ergebnisse der Mikrobiologie Immunitatsforschung und experimentellen Therapie 39, 43–57.Google ScholarPubMed
Levine, N. D., (1971). Taxonomy of the piroplasma. Transactions of the American Microscopical Society 90, 2–33.CrossRefGoogle Scholar
Maclennan, K. J. R., (1957). A staining technique for the identification of trypanosomes in thick blood films. Transactions of the Royal Society of Tropical Medicine and Hygiene 51, 301–2.CrossRefGoogle Scholar
Mettam, A. E., (1907). On the presence of a trypanosome in an Irish bat. Dublin Journal of Medical Science 124, 417–9.CrossRefGoogle Scholar
Molyneux, D. H., & Bafort, J. M., (1971). Observations on the trypanosome of Pipistrellus pipistrellus in Britain, Trypanosoma (Schizotrypanum) vespertilionis. Annales de la Société beige de médicine tropicale 51, 335–48.Google Scholar
Newton, B. A., (1971). DNA of stercorarian trypanosomes. Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 425–6.CrossRefGoogle ScholarPubMed
Paul, J., (1970). Cell and Tissue Culture, ed. 4. Edinburgh and London: Livingstone.Google Scholar
Petana, W. B., (1971). American trypanosomiasis in British Honduras. VIII. The morphogenesis of Trypanosoma (Schizotrypanum) cruzi in the tissues of animals experimentally infected with British Honduras strains of the parasite. Annals of tropical Medicine and Parasitology 65, 21–4.CrossRefGoogle Scholar
Petrie, G. F., (1905). Observations relating to the structure and geographical distribution of certain trypanosomes. Journal of Hygiene, Cambridge 5, 191–200.CrossRefGoogle Scholar
Rodhain, J., (1942). Sur la specificité morphologique et biologique de Trypanosoma pipistrelli, et E. Chatton, R. Courrier. Ada biologica belgica 2, 55–8.Google Scholar
Rodhain, J., & Henry, E., (1942). Sur l'existence de Trypanosoma vespertilionis Battaglia et Trypanosoma pipistrelli Chatton et Courrier chez diverses especes de chauves-souris en Belgique. Acta biologica belgica 2, 261–4.Google Scholar
Rodriguez, E., & Marinkelle, C. J., (1970). Trypanosoma cruzi: development in tissue culture. Experimental Parasitology 27, 78–87.CrossRefGoogle ScholarPubMed
Sergent, Ed., & Sergent, Ét., (1905). Sur des trypanosomes des chauves-souris. Compte rendu des séances de la Société de biologie 58, 53–5.Google Scholar
Southern, H. N., ed. (1964). The Handbook of British Mammals. Oxford: Blackwell Scientific Publications.Google Scholar
Taylor, Angela E. R., & Baker, J. R., (1968). The Cultivation of Parasites in vitro. Oxford and Edinburgh: Blackwell Scientific Publications.Google Scholar
Wenyon, C. M., (1926). Protozoology. London: Baillière, Tindall and Cox.Google Scholar
Zuckerman, Avivah, (1945). In vitro opsonic tests with Plasmodium gallinaceum and Plasmodium lophurae. Journal of Infectious Diseases 77, 28–58.CrossRefGoogle Scholar