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Trypanosoma cruzi heparin-binding proteins mediate the adherence of epimastigotes to the midgut epithelial cells of Rhodnius prolixus

Published online by Cambridge University Press:  07 February 2012

F. O. R. OLIVEIRA -Jr ,
C. R. ALVES ,
F. SOUZA-SILVA ,
C. M. CALVET ,
L. M. C. CÔRTES ,
M. S. GONZALEZ ,
L. TOMA ,
R. I. BOUÇAS ,
H. B. NADER  and
M. C. S. PEREIRA
F. O. R. OLIVEIRA -Jr
Affiliation:
Laboratório de Ultra-estrutura Celular, Instituto Oswaldo Cruz/FIOCRUZ, RJ, Brazil
C. R. ALVES
Affiliation:
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/FIOCRUZ, RJ, Brazil
F. SOUZA-SILVA
Affiliation:
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/FIOCRUZ, RJ, Brazil
C. M. CALVET
Affiliation:
Laboratório de Ultra-estrutura Celular, Instituto Oswaldo Cruz/FIOCRUZ, RJ, Brazil
L. M. C. CÔRTES
Affiliation:
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/FIOCRUZ, RJ, Brazil
M. S. GONZALEZ
Affiliation:
Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal Fluminense, RJ, Brazil
L. TOMA
Affiliation:
Departamento de Bioquímica, Universidade Federal de São Paulo, UNIFESP, SP, Brazil
R. I. BOUÇAS
Affiliation:
Departamento de Bioquímica, Universidade Federal de São Paulo, UNIFESP, SP, Brazil
H. B. NADER
Affiliation:
Departamento de Bioquímica, Universidade Federal de São Paulo, UNIFESP, SP, Brazil
M. C. S. PEREIRA*
Affiliation:
Laboratório de Ultra-estrutura Celular, Instituto Oswaldo Cruz/FIOCRUZ, RJ, Brazil
*
*Corresponding author: Laboratório de Ultra-estrutura Celular, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, Manguinhos, 21040-900 Rio de Janeiro, RJ, Brazil. Tel: +55 21 2598 4330. Fax: +55 21 2598 4330. E-mail: [email protected]
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Summary

Heparin-binding proteins (HBPs) have been demonstrated in both infective forms of Trypanosoma cruzi and are involved in the recognition and invasion of mammalian cells. In this study, we evaluated the potential biological function of these proteins during the parasite-vector interaction. HBPs, with molecular masses of 65·8 kDa and 59 kDa, were isolated from epimastigotes by heparin affinity chromatography and identified by biotin-conjugated sulfated glycosaminoglycans (GAGs). Surface plasmon resonance biosensor analysis demonstrated stable receptor-ligand binding based on the association and dissociation values. Pre-incubation of epimastigotes with GAGs led to an inhibition of parasite binding to immobilized heparin. Competition assays were performed to evaluate the role of the HBP-GAG interaction in the recognition and adhesion of epimastigotes to midgut epithelial cells of Rhodnius prolixus. Epithelial cells pre-incubated with HBPs yielded a 3·8-fold inhibition in the adhesion of epimastigotes. The pre-treatment of epimastigotes with heparin, heparan sulfate and chondroitin sulfate significantly inhibited parasite adhesion to midgut epithelial cells, which was confirmed by scanning electron microscopy. We provide evidence that heparin-binding proteins are found on the surface of T. cruzi epimastigotes and demonstrate their key role in the recognition of sulfated GAGs on the surface of midgut epithelial cells of the insect vector.

Keywords

heparin-binding proteinsTrypanosoma cruziepimastigotesRhodnius prolixus parasite-vector interaction
Type
Research Article
Information
Parasitology , Volume 139 , Issue 6 , May 2012 , pp. 735 - 743
Copyright
Copyright © Cambridge University Press 2012

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