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Trypanosoma caninum n. sp. (Protozoa: Kinetoplastida) isolated from intact skin of a domestic dog (Canis familiaris) captured in Rio de Janeiro, Brazil

Published online by Cambridge University Press:  16 February 2009

M. F. MADEIRA ,
M. A. SOUSA ,
J. H. S. BARROS ,
F. B. FIGUEIREDO ,
A. FAGUNDES ,
A. SCHUBACH ,
C. C. DE PAULA ,
B. N. S. FAISSAL ,
T. S. FONSECA  and
H. K. THOMA
...Show all authors
M. F. MADEIRA*
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
M. A. SOUSA
Affiliation:
Coleção de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
J. H. S. BARROS
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
F. B. FIGUEIREDO
Affiliation:
Laboratório de Pesquisa Clínica em Dermatozooses em Animais Domésticos, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
A. FAGUNDES
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
A. SCHUBACH
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
C. C. DE PAULA
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
B. N. S. FAISSAL
Affiliation:
Coleção de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
T. S. FONSECA
Affiliation:
Coleção de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
H. K. THOMA
Affiliation:
Laboratório de Diagnóstico Molecular e Hematologia, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, RJ, Brasil
M. C. A. MARZOCHI
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
*
*Corresponding author: Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil. Tel: +55 21 3865 9541. Fax: +55 21 3865 9541. E-mail: [email protected]
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Summary

An unknown Trypanosoma species was isolated from an axenic culture of intact skin from a domestic dog captured in Rio de Janeiro, Brazil, which was co-infected with Leishmania (Viannia) braziliensis. Giemsa-stained smears of cultures grown in different media revealed the presence of epimastigotes, trypomastigotes, spheromastigotes, transitional stages, and dividing forms (epimastigotes or spheromastigotes). The highest frequency of trypomastigotes was observed in RPMI (15·2%) and DMEM (9·2%) media containing 5% FCS, with a mean length of these forms of 43·0 and 36·0 μm, respectively. Molecular analysis by sequential application of PCR assays indicated that this trypanosome differs from Trypanosoma cruzi and T. rangeli when specific primers were applied. On the other hand, a PCR strategy targeted to the D7 domain of 24sα rDNA, using primers D75/D76, amplified products of about 250 bp in that isolate (stock A-27), different from the amplification products obtained with T. cruzi and T. rangeli. This organism differs from T. cruzi mainly by the size of its trypomastigote forms and kinetoplasts and the absence of infectivity for macrophages and triatomine bugs. It is also morphologically distinct from salivarian trypanosomes reported in Brazil. Isoenzyme analysis at 8 loci demonstrated a very peculiar banding pattern clearly distinct from those of T. rangeli and T. cruzi. We conclude that this isolate is a new Trypanosoma species. The name T. caninum is suggested.

Keywords

Trypanosoma caninum n. sp.dogtrypanosomeisoenzymespolymerase chain reaction (PCR)Brazil
Type
Research Article
Information
Parasitology , Volume 136 , Issue 4 , April 2009 , pp. 411 - 423
Copyright
Copyright © 2009 Cambridge University Press

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References

REFERENCES

Alvar, J., Cañavate, C., Molina, R., Moreno, J. and Nieto, J. (2004). Canine leishmaniasis. Advances in Parasitology 57, 1–88. doi: 10.1016/S0065-308X(04)57001-X.CrossRefGoogle ScholarPubMed
Bastrenta, B., Mita, N., Buitrago, R., Vargas, F., Flores, M., Machane, M., Yacsik, N., Torrez, M., Le Pont, F. and Brenière, F. (2003). Human mixed infections of Leishmania spp. and Leishmania-Trypanosoma cruzi in a Sub Andean Bolivian Area: identification by polymerase chain reaction/hybridization and isoenzyme. Memórias do Instituto Oswaldo Cruz 98, 255–264. doi: 10.1590/S0074-02762003000200015.CrossRefGoogle Scholar
Beard, C. B., Pye, G., Steurer, F. J., Rodriguez, R., Campman, R., Peterson, A. T., Ramsey, J., Wirtz, R. A. and Robinson, L. E. (2003). Chagas disease in a domestic transmission cycle, Southern Texas, USA. Emerging Infectious Disease 9, 103–105.CrossRefGoogle Scholar
Caballero, Z. C., Sousa, O. E., Marques, W. P., Saez-Alquezar, A. and Umezawa, E. S. (2007). Evaluation of serological tests to identify Trypanosoma cruzi infection in humans and determine cross-reactivity with Trypanosoma rangeli and Leishmania spp. Clinical and Vaccine Immunology 14, 1045–1049. doi: 10.1128/CVI.00127-07.CrossRefGoogle ScholarPubMed
Coutinho, M. T. Z., Bueno, L. L., Sterzik, A., Fujiwara, R. T., Botelho, J. R., De Maria, M., Genaro, O. and Linardi, P. M. (2005). Participation of Rhipicephalus sanguineous (Acari: Ixodidae) in the epidemiology of canine visceral leishmaniasis. Veterinary Parasitology 128, 149–155. doi: 10.1016/j.vetpar.2004.11.011.CrossRefGoogle Scholar
Cupolillo, E., Grimaldi, G. Jr. and Momen, H. (1994). A general classification of New World Leishmania using numerical zymotaxonomy. American Journal of Tropical Medicine and Hygiene 50, 296–311.CrossRefGoogle ScholarPubMed
D'Alessandro, A. (1976). Biology of Trypanosoma (Herpetosoma) rangeli, Tejera, 1920. In The Biology of the Kinetoplastida (ed. Lumsden, W. H. R. and Evans, D. A.), pp. 327–403. Academic Press, New York, USA.Google Scholar
Dantas-Torres, F. (2007). The role of dogs as reservoir of Leishmania parasites, with emphasis on Leishmania (Leishmania) infantum and Leishmania (Viannia) braziliensis. Veterinary Parasitology 149, 139–146. doi:10.1016/j.vetpar.2007.07.007.CrossRefGoogle ScholarPubMed
Deane, L. M. (1964). Animal reservoirs of Trypanosoma cruzi in Brazil. Revista Brasileira de Malariologia e Doenças Tropicais 16, 27–48.Google ScholarPubMed
Dias, E. and Freitas-Filho, L. (1943). Introdução ao estudo biométrico dos hemoflagelados do gênero Schizotrypanum. I. Introdução, material e técnica, problema e métodos estatísticos. Memórias do Instituto Oswaldo Cruz 38, 427–436.CrossRefGoogle Scholar
Fernandes, O., Santos, S. S., Cupolillo, E., Mendonça, B., Derre, R., Junqueira, A. C. V., Santos, L. C., Sturm, N. R., Naiff, R. D., Barret, T. V., Campbell, D. A., Coura, J. R. (2001). A mini-exon multiplex polymerase chain reaction to distinguish the major groups of Trypanosoma cruzi and Trypanosoma rangeli in the Brazilian Amazon. Transactions of the Royal Society of Tropical Medicine and Hygiene 95, 97–99. doi:10.1016/S0035-9203(01)90350-5.CrossRefGoogle ScholarPubMed
Franke, C. R., Greiner, M. and Mehlitz, D. (1994). Investigations on naturally occurring Trypanosoma evansi infections in horses, cattle, dogs and capybaras (Hydrochaeris hydrochaeris) in Pantanal de Poconé (Mato Grosso, Brazil). Acta Tropica 58, 159–169. doi:10.1016/0001-706X(94)90055-8.CrossRefGoogle ScholarPubMed
Githure, J. I., Anjili, C. O., Ngumbi, P. M., Mwanyumba, P. M., Lugalia, R., Koech, D. K. and Kinoti, G. K. (1995). Isolation and characterization of flagellates from rodents and canids in Masting, Machakos District. Kenya. African Journal of Health Sciences 2, 372–375.Google Scholar
Gürtler, R. E., Cécere, M. C., Lauricella, M. A., Cardinal, M. V., Kitron, U. and Cohen, J. E. (2007). Domestic dogs and cats as sources of Trypanosoma cruzi infection in rural northwestern Argentina. Parasitology 134, 69–82. doi: 10.1017/S0031182006001259.CrossRefGoogle ScholarPubMed
Harrus, S., Harmelin, A., Presenty, B. and Bark, H. (1995). Trypanosoma congolense infection in two dogs. Journal of Small Animal Practice 36, 83–86.CrossRefGoogle ScholarPubMed
Hoare, C. A. (1972). The Trypanosomes of Mammals. A Zoological Monograph. Blackwell Scientific Publications, Oxford-Edinburgh, UK.Google Scholar
Kaggwa, E., Munyua, W. K. and Mugera, G. M. (1984). The pathogenicity of Trypanosoma brucei brucei in dogs. Bulletin of Animal Health and Production in Africa 82, 360–368.Google Scholar
Lainson, R., Da Silva, F. M. and Franco, C. M. (2008). Trypanosoma (Megatrypanum) saloboense n. sp. (Kinetoplastida: Trypanosomatidae) parasite of Monodelphis emiliae (Marsupiala: Didelphidae) from Amazonian Brazil. Parasite 15, 99–103.CrossRefGoogle Scholar
Madeira, M. F., Schubach, A. O., Schubach, T. M. P., Pacheco, R. S., Oliveira, F. S., Pereira, S. A., Figueredo, F. B., Baptista, C. and Marzochi, M. C. A. (2006 a). Mixed infection with Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in a naturally infected dog from Rio de Janeiro, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 100, 442–445. doi: 10.1016/j.trstmh.2005.07.011.CrossRefGoogle Scholar
Madeira, M. F., Schubach, A. O., Schubach, T. M. P., Pereira, S. A., Figueredo, F. B., Baptista, C., Leal, C. A., Melo, C. X., Confort, E. M. and Marzochi, M. C. A. (2006 b). Post mortem parasitological evaluation of dogs seroreactive for Leishmania from Rio de Janeiro, Brazil. Veterinary Parasitolology 138, 366–370. doi: 10.1016/j.vetpar.2006.01.059.CrossRefGoogle Scholar
Madeira, M. F., Schubach, A. O., Schubach, T. M. P., Serra, C. M. B., Pereira, S. A., Figueiredo, F. B., Confort, E. M., Quintella, L. P. and Marzochi, M. C. A. (2005). Is Leishmania (Viannia) braziliensis preferentially restricted to the cutaneous lesions of naturally infected dogs? Parasitology Research 97, 73–76. doi: 10.1007/s00436-005-1374-y.CrossRefGoogle Scholar
Madeira, M. F., Serra, C. M. B., Schubach, T. M. P., Pereira, S., Figueredo, F. B., Soares, M. J., Quintella, L. P., Schubach, A. and Marzochi, M. C. A. (2004). Co-infecção natural por Leishmania (Viannia) braziliensis e Trypanosoma sp. em cão doméstico. Revista da Sociedade Brasileira de Medicina Tropical 37 (Suppl.), 217.Google Scholar
Marzochi, M. C. A., Coutinho, S. G., Souza, W. J. S., Toledo, L. M., Grimaldi, G. Jr., Momen, H., Pacheco, R. S., Sabroza, P. C., Souza, M. A., Rangel, F. B. and Tramontano, N. C. (1985). Canine visceral leishmaniasis in Rio de Janeiro, Brazil. Clinical, parasitological, therapeutical and epidemiological findings (1977–1983). Memórias do Instituto Oswaldo Cruz 80, 349–357.CrossRefGoogle Scholar
Ministério, da Saúde (2006). Manual de vigilância e controle da Leishmaniose visceral, 1st Ed. Ministério da Saúde, Brasília.Google Scholar
Montenegro, V. M., Jiménez, M., Dias, J. C. P. and Zeledón, R. (2002). Chagas disease in dogs from endemic areas of Costa Rica. Memórias do Instituto Oswaldo Cruz 97, 491–494. doi: 10.1590/S0074-02762002000400006.CrossRefGoogle ScholarPubMed
Otto, T. D., Vasconcellos, E. A., Gomes, L. H., Moreira, A. S., Degrave, W. M., Mendonça-Lima, L. and Alves-Ferreira, M. (2008). ChromaPipe: a pipeline for analysis, quality control and management for a DNA sequencing facility. Genetics and Molecular Research 23, 861–871.CrossRefGoogle Scholar
Pifano, F., Peñalver, L. M., Medina, R. and Dominguez, E. (1948). La infección natural del perro por el Trypanosoma rangeli. Primeira comprobación de un reservorio extrahumano vertebrado del protozoario en la naturaleza. Gaceta Médica 56, 130–134.Google Scholar
Reithinger, R. and Davies, C. R. (1999). Is the domestic dog (Canis familiaris) a reservoir host of American cutaneous leishmaniasis? A critical review of the current evidence. American Journal of Tropical Medicine and Hygiene 61, 530–541.CrossRefGoogle ScholarPubMed
Saldaña, A. and Souza, O. E. (1996). Trypanosoma rangeli: epimastigote immunogenecity and cross-reaction with Trypanosoma cruzi. Journal of Parasitology 82, 363–366.CrossRefGoogle Scholar
Savani, E. S. M. M., Nunes, V. L. B., Galati, E. A. B., Castilho, T. M., Araújo, F. S., Ilha, I. M. N., Camargo, M. C. G. O., D'Auria, S. R. N. and Floeter-Winter, L. M. (2005). Occurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the State of Mato Grosso do Sul, Brazil. Memórias do Instituto Oswaldo Cruz 100, 739–741. doi: 10.1590/S0074-02762005000700011.CrossRefGoogle Scholar
Shastri, U. V. and Deshpande, P. D. (1981). Hyalomma anatolicum (Kock, 1844) as a possible vector for transmission of Trypanosoma theileri, Laveran, 1902 in cattle. Veterinary Parasitology 9, 151–155. doi: 10.1016/0304-4017(81)90034-0.CrossRefGoogle Scholar
Sousa, M. A., Fonseca, T. S., Santos, B. N., Santos-Pereira, S., Carvalhal, C. and Hasslocher-Moreno, A. M. (2008). Trypanosoma rangeli Tejera, 1920, in chronic Chagas' disease patients under ambulatory care at the Evandro Chagas Clinical Research Institute (IPEC – Fiocruz, Brazil). Parasitology Research 103, 697–703. doi: 10.1007/s00436-008-1033-1.CrossRefGoogle Scholar
Souto, R. P. and Zingales, B. (1993). Sensitive detection and strain classification of Trypanosoma cruzi by amplification of a ribosomal RNA sequence. Molecular and Biochemical Parasitology 62, 45–52. doi:10.1016/0166-6851(93)90176-X.CrossRefGoogle ScholarPubMed
Souto, R. P., Vargas, N. and Zingales, B. (1999). Trypanosoma rangeli: Discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene. Experimental Parasitology 91, 306–314. doi:10.1006/expr.1998.4380.CrossRefGoogle ScholarPubMed
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599. doi:10.1093/molbev/msm092.CrossRefGoogle ScholarPubMed
Taylor, A. E. R. and Baker, J. R. (1968). The Cultivation of Parasites ‘In Vitro’, Trypanosomatidae. Blackwell Scientific Publications, Oxford-Edinburgh, UK.Google Scholar
Vargas, N., Souto, R. P., Carranza, J. C., Vallejo, G. A. and Zingales, B. (2000). Amplification of a specific repetitive DNA sequence for Trypanosoma rangeli identification and its potential application in epidemiological investigations. Experimental Parasitology 96, 147–159. doi: 10.1006/expr.2000.4563.CrossRefGoogle ScholarPubMed
Vickerman, K. (1976). The diversity of the kinetoplastid flagellates. In The Biology of the Kinetoplastida (ed. Lumsden, W. H. R. and Evans, D. A.), pp. 1–34. Academic Press, New York, USA.Google Scholar
Vickerman, K. (1965). Polymorphism and mitochondrial activity in sleeping sickness trypanosomes. Nature, London 208, 762–766.CrossRefGoogle ScholarPubMed
Wilson, M. E. (2002). Prevention of tick-borne diseases. The Medical Clinics of North America 86, 219–238.CrossRefGoogle ScholarPubMed
Wincker, P., Britto, C., Pereira, J. B., Cardoso, M. A., Oelemann, W. and Morel, C. M. (1994). Use of a simplified polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients in a rural endemic area. Americal Journal of Tropical Medicine and Hygiene 51, 771–777.CrossRefGoogle Scholar
Ziccardi, M. and Lourenço-de-Oliveira, R. (1998). Morphological features of trypanosomes from squirrel monkeys from Brazilian Amazon. Memórias do Instituto Oswaldo Cruz 93, 45–55.CrossRefGoogle ScholarPubMed