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Trypanocidal activity of blood and tissue fluid from normal and infected rabbits treated with curative drugs

Published online by Cambridge University Press:  06 April 2009

L. G. Goodwin  and
Ellen D. Tierney
L. G. Goodwin
Affiliation:
Nuffield Institude of Comparative Medicine, Zoological Society of London, Regenťs Park, London N W1 4RY
Ellen D. Tierney
Affiliation:
Nuffield Institude of Comparative Medicine, Zoological Society of London, Regenťs Park, London N W1 4RY
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Extract

A new technique for determining the trypanocidal activity of body fluids has been devised, using very small quantities in the wells of plastic microtest plates.

The activities of blood and tissue fluid of rabbits have been measured after the injection of melarsoprol, suramin, diminazene aceturate and isometamidium chloride. Activity against Trypanosoma (Trypanozoon)brucei Persisted in body fluids for a shorter time in treated normal rabbits than in treated rabbits infected with the tryponosome. The difference was caused by the participation of the immune response of the infected animals.

The new technique was sensitive enough to detect these interactions of chemotherapy and immunology and should be of use in the study of new trypanocidal compounds.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 1 , February 1977 , pp. 33 - 45
Copyright
Copyright © Cambridge University Press 1977

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