Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-23T16:11:02.743Z Has data issue: false hasContentIssue false
  • English
  • Français

Trichinella spiralis: immunocytochemical localization of surface and intracellular antigens using monoclonal antibody probes

Published online by Cambridge University Press:  06 April 2009

Diane J. McLaren ,
Guadalupe Ortega-Pierres  and
R. M. E. Parkhouse
Diane J. McLaren
Affiliation:
Divisions of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Guadalupe Ortega-Pierres
Affiliation:
Department of Genetics, Centro De Investigación y de Estudios Avanzados IPN, Aparto Postal 14-740, 0700 Mexico DF, Mexico
R. M. E. Parkhouse
Affiliation:
Divisions of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Summary

A panel of immunologically and biochemically defined monoclonal antibody probes has been used in conjunction with immunocytochemical techniques to localize target antigens in sections of different life-cycle stages of Trichinella spiralis. Monoclonals that immunoprecipitate surface components from adult worms, show reactivity with the surface but not with internal tissues of sectioned parasites. Reagents that immunoprecipitate radio-isotope labelled stage-specific surface components of muscle-stage larvae, however, react with the stichosome and gut lining of sectioned larvae, as well as with the surface. Monoclonal antibody probes that do not stain the surfaces of live, intact muscle larvae in immunofluorescence assays, but which immunoprecipitate solubilized surface glycoproteins, also show reactivity with cuticular and stichosomal antigens of sectioned larvae. The more powerful resolution provided by electron microscopy has localized the surface antigens to the epicuticle and the intestinal antigens to the brush-border microvilli. Of particular interest was the finding that antigens of muscle-stage larvae, known to confer protection upon recipient mice, also exist in the stichosome of adult parasites. This observation may shed some light on the fact that mice immunized with antigens from muscle-stage larvae show, in addition to reduced muscle larva burden, accelerated expulsion of adult worms. The implications of these data for stage specificity of immune responses to trichinosis are discussed.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 1 , February 1987 , pp. 101 - 114
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Almond, N. M., McLaren, D. J. & Parkhouse, R. M. E. (1986). A comparison of the surface and secretions of Trichinella pseudospiralis and Trichinella spiralis. Parasitology 93,CrossRefGoogle Scholar
Bruce, R. (1970). Structure of the oesophagus of the infective juvenile and adult Trichinella spiralis. Journal of Parasitology 56, 540–9.CrossRefGoogle ScholarPubMed
Capo, V., Despommier, D. D. & Silberstein, D. S. (1984). The site of ecdysis of the L1 larva of Trichinella spiralis. Journal of Parasitology 70, 992–4.Google Scholar
Chitwood, M. B. & Chitwood, B. G. (1950). An Introduction to Nematology. Baltimore: Monumental.Google Scholar
Dennis, D. T., Despommier, D. D. & Davis, N. (1970). Infectivity of the newborn larva of Trichinella spiralis in the rat. Journal of Parasitology 56, 974–7.Google Scholar
Despommier, D. D. & Muller, M. (1976). The stichosome and its secretion granules in the mature muscle larva of Trichinella spiralis. Journal of Parasitology 62, 775–85.CrossRefGoogle ScholarPubMed
Gamble, H. R. (1985 a). Trichinella spiralis: immunization of mice using monoclonal antibody affinity-isolated antigens. Experimental Parasitology 59, 398–404.CrossRefGoogle ScholarPubMed
Gamble, H. R. (1985 b). Comparison of the immune effects in mice immunized with Trichinella spiralis adult and larval antigens. Journal of Parasitology 71, 680–2.Google Scholar
Lee, D. L., Wright, K. A. & Shivers, R. R. (1984). A freeze fracture study of the surface of the infective-stage larva of the nematode Trichinella. Tissue and Cell 16, 819–28.CrossRefGoogle ScholarPubMed
McLaren, D. J. (1980). Schistosoma mansoni: the parasite surface in relation to host immunity. In Tropical Medicine Research Studies, vol. 1 (ed. Brown, K. N.). Tropical Medicine Research Studies Press, Chichester: John Wiley.Google Scholar
McLaren, D. J. (1984). Disguise as an evasive stratagem of parasitic organisms. In Parasite Evasion of the Immune Response. Symposia of the British Society for Parasitology, vol. 21. Parasitology 88, 597–612.Google Scholar
Ortega-Pierres, G., Chayen, A., Clark, N. W. T. & Parkhouse, R. M. E. (1984 a). The occurrence of antibodies to hidden and exposed determinants of surface antigens of Trichinella spiralis. Parasitology 88, 359–69.CrossRefGoogle ScholarPubMed
Ortega-Pierres, G., Clark, N. W. T. & Parkhouse, R. M. E. (1986). Regional specialisation of the surface of a parasitic nematode. Parasite Immunology (in the Press).Google Scholar
Ortega-Pierres, G., Mackenzie, C. D. & Parkhouse, R. M. E. (1984 b). Protection against Trichinella spiralis induced by a monoclonal antibody that promotes killing of newborn larvae by granulocytes. Parasite Immunology 6, 275–84.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E., Philipp, M. & Ogilvie, B. M. (1981). Surface antigens of Trichinella spiralis infective larvae. Parasite Immunology 3, 339–52.CrossRefGoogle ScholarPubMed
Philipp, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1980). Changing proteins on the surface of parasitic nematodes. Nature, London 287, 538–40.CrossRefGoogle Scholar
Pritchard, D. I. (1977). Immunology of trichinosis in the guinea pig. The role of immediate hypersensitivity. Ph.D. thesis, University of Birmingham.Google Scholar
Pritchard, D. I. (1985). Antigen production by encysted muscle larvae of Trichinella spiralis. Journal of Helminthology 59, 71–7.CrossRefGoogle ScholarPubMed
Purkerson, J. & Despommier, D. D. (1974). In Trichinellosis, (ed. Kim, C. W.), pp. 7–24. New York: Intext Publishers.Google Scholar
Silberstein, D. S. & Despommier, D. D. (1984). Antigens from Trichinella spiralis that induce a protective response in the mouse. Journal of Immunology 132, 898–904.Google Scholar
Silberstein, D. S. & Despommier, D. D. (1985). Effects on Trichinella spiralis of host responses to purified antigens. Science 227, 948–50.CrossRefGoogle ScholarPubMed
Theiler, H., Augustine, D. L. & Spink, W. W. (1935). On the persistence of eosinophilia and on immune reactions in human trichinosis, several years after recovery. Parasitology 27, 345–54.Google Scholar