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Tracking radioactive larvae of Strongyloides ratti in the host

Published online by Cambridge University Press:  06 April 2009

P. A. G Wilson
P. A. G Wilson
Affiliation:
University of Edinburgh, Department of Zoology, West Mains Road, Edinburgh EH9 3JT
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Summary

Infective larvae of homogonic Strongyloides ratti grown in faecal culture with 32P or 75Se acquired a significant amount of radioactivity which was firmly attached to them. Heating removed most of the 32P but left 75Se in place. Subcutaneous injection of virgin and nursing mother rats with living and heat-killed radioactive larvae resulted in a pattern of labelling in the small intestine of injected animals and, in the case of 75Se, those of suckling pups, which can only be explained if labelled worms follow the natural migratory routes. The use of this tool in migratory studies is discussed, with precautions to allow for flaws in the technique.

Type
Research Article
Information
Parasitology , Volume 79 , Issue 1 , August 1979 , pp. 29 - 38
Copyright
Copyright © Cambridge University Press 1979

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References

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