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Toxoplasma gondii-structure variations of the antigen P30

Published online by Cambridge University Press:  06 April 2009

A. Bonhomme ,
C. Thirion ,
F. Boulanger ,
F. Charton ,
H. Burlet ,
J. M. Pinon  and
A. J. P. Alix
A. Bonhomme
Affiliation:
Laboratoire de Parasitologie, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
C. Thirion
Affiliation:
Laboratoire de Spectroscopie et Structures Biomoléculaires, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
F. Boulanger
Affiliation:
Laboratoire de Parasitologie, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
F. Charton
Affiliation:
Laboratoire de Spectroscopie et Structures Biomoléculaires, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
H. Burlet
Affiliation:
Laboratoire de Parasitologie, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
J. M. Pinon
Affiliation:
Laboratoire de Parasitologie, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
A. J. P. Alix
Affiliation:
Laboratoire de Spectroscopie et Structures Biomoléculaires, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
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Summary

The major surface immunodominant antigen (P30) of Toxoplasma gondii was purified by two methods (i) SDS–PAGE and (ii) immunoaffinity chromatography. The secondary elements within this protein were assessed by circular dichroism and spectra obtained were compared to those proposed by Manavalan & Johnson (1983). The results allowed us to determine an all β protein status for this antigen. This experimental result was in agreement with the predicted secondary structures deduced from the P30 primary sequence. Modifications in conformation according to pH and temperature were recorded without any change in immunoactivity. The epitope, which was always recognized by a monoclonal antibody against P30, could be a linear epitope.

Keywords

Toxoplasma gondiiantigen P30circular dichroismmolecular conformation
Type
Research Article
Information
Parasitology , Volume 108 , Issue 3 , April 1994 , pp. 281 - 287
Copyright
Copyright © Cambridge University Press 1994

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References

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