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Toxoplasma gondii induces changes in intracellular calcium in macrophages

Published online by Cambridge University Press:  04 September 2007

K. S. MASEK ,
P. ZHU ,
B. D. FREEDMAN  and
C. A. HUNTER
K. S. MASEK
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Room 313, Hill Pavilion, 380 South University Avenue, University of Pennsylvania, Philadelphia, PA 19104, USA
P. ZHU
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Room 313, Hill Pavilion, 380 South University Avenue, University of Pennsylvania, Philadelphia, PA 19104, USA
B. D. FREEDMAN
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Room 313, Hill Pavilion, 380 South University Avenue, University of Pennsylvania, Philadelphia, PA 19104, USA
C. A. HUNTER*
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Room 313, Hill Pavilion, 380 South University Avenue, University of Pennsylvania, Philadelphia, PA 19104, USA
*
*Corresponding author. Tel: +215 573 7772. E-mail: [email protected]
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Summary

Toxoplasma gondii is an obligate intracellular parasite that interacts with calcium storage organelles and induces calcium-dependent signalling in macrophages. This study was performed to determine whether Toxoplasma induces changes in intracellular calcium in these cells. Ratiometric imaging of live, Fura-2 loaded macrophages challenged with T. gondii revealed robust elevations in intracellular calcium. These elevations were late in onset, beginning 15–20 min after addition of parasites and occurred in up to 20% of macrophages in an imaging field. Further characterization of these events revealed that they follow from challenge with live T. gondii, but not heat-killed parasites or soluble Toxoplasma antigen (STAg). Parasite-induced calcium elevations derived from extracellular sources, and were independent of host recognition factors MyD88 and CCR5. These findings indicate that Toxoplasma gondii alters calcium homeostasis in macrophages and this activity is independent of known pathways involved in the innate recognition of this organism.

Keywords

Toxoplasma gondiicalciummacrophage/monocyte
Type
Research Article
Information
Parasitology , Volume 134 , Issue 14 , December 2007 , pp. 1973 - 1979
Copyright
Copyright © Cambridge University Press 2007

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