Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-24T11:46:41.919Z Has data issue: false hasContentIssue false
  • English
  • Français

Thymidine uptake by chicken kidney cells parasitized by Eimeria tenella: evidence for a diffusable mediating substance

Published online by Cambridge University Press:  06 April 2009

Catriona Urquhart
Catriona Urquhart
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London
Get access Rights & Permissions [Opens in a new window]

Summary

The pattern of DNA synthesis in chicken kidney (CK) cells parasitized by Eimeria tenella was altered; a 2-fold increase in the uptake of [3H]-thymidine occurred in these cells compared with that shown by the controls. This difference was significantly different at 16 h post-inoculation (p.i.) and reached a peak at 20 h p.i. Non-parasitized cells in co-culture with parasitized cells showed a level of incorporation intermediate between that of the parasitized and non-parasitized mono- cultures. It was shown that this stimulation was due to a factor released into the medium by the parasite or the parasitized cell. A possible course of events leading to induced and prolonged DNA synthesis by the host cell and the significance of this change in the life-cycle of the parasite is discussed.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 2 , April 1981 , pp. 189 - 194
Copyright
Copyright © Cambridge University Press 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Beyer, T. V. & Shibalova, T. A. (1974). The increase of DNA quantity in the nuclei of chicken caecal cells harbouring second generation schizonts of Eimeria tenella. Parasitologia (Leningrad) 8, 449–55. (In Russian.)Google Scholar
Browning, R. F., Patton, W. H. & Lytle, C. F. (1976). Eimeria tenella: Stimulation of DNA synthesis in infected cultured animal cells. Experimental Parasitology 39, 195–203.CrossRefGoogle ScholarPubMed
Fernando, M. A. & Pasternak, J. (1977). Isolation of chick intestinal cells infected with second-generation schizonts of Eimeria necatrix. Parasitology 74, 19–26.CrossRefGoogle ScholarPubMed
Fernando, M. A., Pasternak, J., Barrell, R. & Stockdale, P. H. G. (1974). Induction of host nuclear DNA synthesis in coccidia-infected chicken intestinal cells. International Journal for Parasitology 4, 267–76.CrossRefGoogle ScholarPubMed
Latter, V. S. (1977). Nucleic acid metabolism of Eimeria growing in cultivated cells. 5th International Congress of Protozoology New York, 380.Google Scholar
Morgan, K. & Canning, E. U. (1974). Incorporation of 3H-thymidine and 3H-adenosine by Eimeria tenella grown in chick embryos. Journal of Parasitology 60, 364–7.CrossRefGoogle ScholarPubMed
Ouellette, C. A., Strout, R. G. & McDougald, L. R. (1973). Incorporation of radioactive pyrimidine nucleosides into DNA and RNA of Eimeria tenella (Coccidia) cultured in vitro. Journal of Protozoology 20, 150–3.CrossRefGoogle ScholarPubMed
Ouellette, C. A., Strout, R. G. & McDougald, L. R. (1974). Thymidylic acid synthesis in Eimeria tenella (Coccidia) cultured in vitro. Journal of Protozoology 21, 398–400.CrossRefGoogle ScholarPubMed
Roberts, W. L., Elsner, Y. Y., Shigematsu, A. & Hammond, D. M. (1970). Lack of incorporation of 3H-thymidine in Eimeria callospermophili in cell cultures. Journal of Parasitology 56, 833–4.CrossRefGoogle Scholar
Urquhart, C. M. (1981). Morphological and adhesive changes to cultured chick kidney cells following parasitization with Eimeria tenella (Protozoa: Coccidia). Parasitology 82, 175–87.CrossRefGoogle Scholar