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Thiamine uptake in isolated schizonts of Eimeria tenella and the inhibitory effects of amprolium

Published online by Cambridge University Press:  06 April 2009

S. James
S. James
Affiliation:
Imperial Chemical Industries Ltd, Pharmaceuticals Division, Alderley Park, Macclesfield, Cheshire, SK10 4TG
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Summary

The uptake of thiamine by isolated second-generation schizonts of Eimeria tenella and by host intestinal cells was found to consist of two components. One was passive and the other apparently an active process. The kinetic constants of the latter were compared in host (Km = 0·36 μm) and parasite (Km = 0·07 μm) and found to be significantly different. Both systems were competitively inhibited by amprolium but showed different affinities for the drug. (Host Ki = 326 μm; parasite Ki = 7·6 μm). Further differences were observed in schizonts of a drug-resistant line of E. tenella. These findings are discussed in terms of the inhibition of thiamine uptake being the basis of the anticoccidial activity of amprolium.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 2 , April 1980 , pp. 313 - 322
Copyright
Copyright © Cambridge University Press 1980

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