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Therapeutic efficacy of mebendazole and artemisinin in different phases of trichinellosis: a comparative experimental study

Published online by Cambridge University Press:  13 January 2021

Amal Farahat Allam Open the ORCID record for Amal Farahat Allam [Opens in a new window] ,
Reham Ahmed Mostafa ,
Wael Lotfy ,
Hoda Fahmy Farag ,
Naglaa Fathi ,
Esraa Abdelhamid Moneer  and
Amel Youssef Shehab
Amal Farahat Allam*
Affiliation:
Medical Research Institute, University of Alexandria, Department, Alexandria, Egypt
Reham Ahmed Mostafa
Affiliation:
Department of chemistry, Faculty of Education, Alexandria University, Alexandria, Egypt
Wael Lotfy
Affiliation:
Faculty of Nursing, Matrouh University, Matrouh, Egypt
Hoda Fahmy Farag
Affiliation:
Medical Research Institute, University of Alexandria, Department, Alexandria, Egypt
Naglaa Fathi
Affiliation:
Medical Research Institute, University of Alexandria, Department, Alexandria, Egypt
Esraa Abdelhamid Moneer
Affiliation:
Department of Medical Laboratory, Faculty of Allied Medical Sciences, Pharos University, Alexandria, Egypt
Amel Youssef Shehab
Affiliation:
Medical Research Institute, University of Alexandria, Department, Alexandria, Egypt
*
Author for correspondence: Amal Farahat Allam, E-mail: [email protected]
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Abstract

The present work aimed at studying the efficacy of mebendazole (MBZ) compared to artemisinin (ART) for the treatment of trichinellosis at various phases of infection. Seventy Swiss albino mice were orally infected by 300 Trichinella spiralis (T. spiralis) larvae. Mice were divided into infected untreated control group and infected groups treated with 50 mg kg−1 MBZ and 300 mg kg−1 ART for three and five consecutive days, respectively, at the enteral phase [2–4 days post infection (PI)], invasive phase (10–12 days PI) and encapsulated phase (28–30 days PI). All mice were sacrificed 35–42 days PI. MBZ and ART revealed a significant decrease in mean larval counts and increase of larval per cent reduction (LR %) when treatment was initiated during the enteral phase compared to the other phases. MBZ showed significantly higher LR % (99.7, 83.95 and 89.65%) than ART (80.58, 67.0 and 79.2%) when administered at the three infection phases. Histopathological study showed a decrease in the number of encysted larvae, their surrounding cellular infiltrates and increased regenerative muscles in all treated mice. In conclusion, ART possesses a substantial anthelmintic activity against T. spiralis infection in mice both at the enteral and encapsulated phases, yet, significantly lower than MBZ.

Keywords

Artemisininencysted larvaemebendazolemiceTrichinella spiralis
Type
Research Article
Information
Parasitology , Volume 148 , Issue 5 , April 2021 , pp. 630 - 635
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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