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Surface and species-specific antigens of Schistosoma haematobium

Published online by Cambridge University Press:  06 April 2009

C. Kelly ,
P. Hagan ,
M. Knight ,
J. Hodgson ,
A. J. G. Simpson ,
F. Hackett ,
H. A. Wilkins  and
S. R. Smithers
C. Kelly
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
P. Hagan
Affiliation:
Medical Research Council Laboratories, Fajara, The Gambia, West Africa
M. Knight
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
J. Hodgson
Affiliation:
Medical Research Council Laboratories, Fajara, The Gambia, West Africa
A. J. G. Simpson
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
F. Hackett
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
H. A. Wilkins
Affiliation:
Medical Research Council Laboratories, Fajara, The Gambia, West Africa
S. R. Smithers
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
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Summary

Of the surface antigens identified by radio-iodination, two-dimensional gel analyses showed no similarities between those of Schistosoma haematobium and Schistosoma mansoni, thus providing a basis for the species specificity of these antigens described previously (Simpson, Knight, Hagan, Hodgson, Wilkins & Smithers (1985) Parasitology 90, 499–508). The surface antigens of S. haematobium were glycosylated and comprised an acidic polypeptide of Mr 17000 as well as a complex set of polypeptides of approximate pI 6–7, which resolved in the Mr range 20000–30000. At least one of the lower Mr forms of this complex is also present in the adult worm. Limited cross-reaction was observed with S. mansoni infection sera and this may be due to a shared carbohydrate epitope. In contrast, extensive cross-reaction was observed using sera from mice immunized with S. bovis. This pattern parallels the species-specificity of vaccine-induced immunity. Extensive cross-reaction was also observed within cell-free translation products of m-RNA from adult worms of S. haematobium and S. mansoni by use of heterologous human infection sera. The few antigens which were species-specific may represent surface antigens.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 2 , October 1987 , pp. 253 - 266
Copyright
Copyright © Cambridge University Press 1987

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