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Successive changes in tissue migration capacity of developing larvae of an intestinal nematode, Strongyloides venezuelensis

Published online by Cambridge University Press:  09 November 2005

H. MARUYAMA ,
A. NISHIMAKI ,
Y. TAKUMA ,
M. KURIMOTO ,
T. SUZUKI ,
Y. SAKATOKU ,
M. ISHIKAWA  and
N. OHTA
H. MARUYAMA
Affiliation:
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan
A. NISHIMAKI
Affiliation:
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan
Y. TAKUMA
Affiliation:
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan
M. KURIMOTO
Affiliation:
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan
T. SUZUKI
Affiliation:
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan
Y. SAKATOKU
Affiliation:
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan
M. ISHIKAWA
Affiliation:
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan
N. OHTA
Affiliation:
Department of Molecular Parasitology, Nagoya City University Graduate School of Medical Sciences, Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan
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Abstract

Infective larvae of an intestinal nematode, Strongyloides venezuelensis, enter rodent hosts percutaneously, and migrate through connective tissues and lungs. Then they arrive at the small intestine, where they reach maturity. It is not known how S. venezuelensis larvae develop during tissue migration. Here we demonstrate that tissue invasion ability of S. venezuelensis larvae changes drastically during tissue migration, and that the changes are associated with stage-specific protein expression. Infective larvae, connective tissue larvae, lung larvae, and mucosal larvae were used to infect mice by various infection methods, including percutaneous, subcutaneous, oral, and intraduodenal inoculation. Among different migration stages, only infective larvae penetrated mouse skin. Larvae, once inside the host, quickly lost skin penetration ability, which was associated with the disappearance of an infective larva-specific metalloprotease. Migrating larvae had connective tissue migration ability until in the lungs, where larvae became able to settle down in the intestinal mucosa. Lung larvae and mucosal larvae were capable of producing and secreting adhesion molecules.

Keywords

Strongyloides venezuelensisintestinal nematodemigrationdevelopmentlarva
Type
Research Article
Information
Parasitology , Volume 132 , Issue 3 , March 2006 , pp. 411 - 418
Copyright
© 2005 Cambridge University Press

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