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Subcellular distribution of the Entamoeba histolytica 140 kDa FN-binding molecule during host-parasite interaction

Published online by Cambridge University Press:  01 November 2006

V. I. HERNÁNDEZ-RAMÍREZ ,
A. RIOS ,
A. ANGEL ,
M. A. MAGOS ,
L. PÉREZ-CASTILLO ,
J. L. ROSALES-ENCINA ,
E. CASTILLO-HENKEL  and
P. TALAMÁS-ROHANA
V. I. HERNÁNDEZ-RAMÍREZ
Affiliation:
Superior School of Medicine, IPN, Plan de San Luis y Díaz Mirón, Col. Casco de Sto. Tomás México, D.F., 11340, México
A. RIOS
Affiliation:
Department of Experimental Pathology, CINVESTAV-IPN, Ave. IPN No. 2508, Col. San Pedro Zacatenco, México, D.F., 07360, México
A. ANGEL
Affiliation:
Department of Experimental Pathology, CINVESTAV-IPN, Ave. IPN No. 2508, Col. San Pedro Zacatenco, México, D.F., 07360, México
M. A. MAGOS
Affiliation:
Department of Genetics and Molecular Biology, CINVESTAV-IPN, Ave. IPN No. 2508, Col. San Pedro Zacatenco, México, D.F., 07360, México
L. PÉREZ-CASTILLO
Affiliation:
Department of Experimental Pathology, CINVESTAV-IPN, Ave. IPN No. 2508, Col. San Pedro Zacatenco, México, D.F., 07360, México
J. L. ROSALES-ENCINA
Affiliation:
Department of Experimental Pathology, CINVESTAV-IPN, Ave. IPN No. 2508, Col. San Pedro Zacatenco, México, D.F., 07360, México
E. CASTILLO-HENKEL
Affiliation:
Superior School of Medicine, IPN, Plan de San Luis y Díaz Mirón, Col. Casco de Sto. Tomás México, D.F., 11340, México
P. TALAMÁS-ROHANA
Affiliation:
Department of Experimental Pathology, CINVESTAV-IPN, Ave. IPN No. 2508, Col. San Pedro Zacatenco, México, D.F., 07360, México
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Abstract

Entamoeba histolytica trophozoites recovered from the host-parasite interface during abscess development obtain different stimuli compared with long-term cultured cells. In order to have a better understanding about the mechanisms in which the 140 kDa fibronectin (FN)-binding molecule (EhFNR) is involved during the invasive process, we decided to compare the regulation process of this molecule among long-term cultured trophozoites, FN-stimulated trophozoites, and trophozoites recently recovered from a liver abscess. A cDNA clone (5A) containing a fragment of the EhFNR that shows identity to the C-terminal region of the intermediate galactose lectin subunit Igl, was selected with a mAb (3C10). Identity of EhFNR with Igl was confirmed by immunoprecipitation with 3C10 and EH3015 (against the Gal/GalNAc intermediate subunit) mAbs. The 3C10 mAb was used as a tool to explore the modulation of the amoebic receptor (EhFNR). Our results showed specific regulation of the EhFNR in FN-interacted amoebas, as well as in trophozoites recovered at different stages of abscess development. This regulation involved mobilization of the receptor molecule from internal vesicles to the plasma membrane. Therefore, we suggest that in the host-parasite interface, the EhFNR (Igl) plays an important role in the adhesion process during abscess development.

Keywords

Entamoeba histolyticafibronectinfibronectin-binding moleculehost-parasite interfaceGal/GalNAc intermediate subunitvesicle mobilization
Type
Research Article
Information
Parasitology , Volume 134 , Issue 2 , February 2007 , pp. 169 - 177
Copyright
2006 Cambridge University Press

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