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A study of carbohydrate metabolism in the eggs (oncospheres) of the tapeworm, Hymenolepis diminuta, with special reference to glucose

Published online by Cambridge University Press:  06 April 2009

P. W. Pappas  and
G. M. Durka
P. W. Pappas
Affiliation:
Department of Zoology, The Ohio State University, 1735 Neil Avenue, Columbus, OH 43210–1293, USA
G. M. Durka
Affiliation:
Department of Zoology, The Ohio State University, 1735 Neil Avenue, Columbus, OH 43210–1293, USA
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Summary

When incubated in vitro for 24 h, intact eggs, chemically shelled eggs (obtained by treating intact eggs with NaOCl), activated larvae (eggs in which the outer shell and inner envelope were removed), and oncospheres (activated larvae treated with papain to remove the embryophore) absorb and metabolize radioactive glucose. Intact eggs, which are covered by the impermeable shell, absorb only small amounts of exogenous radioactive glucose, while chemically shelled eggs, activated larvae, and oncospheres absorb much larger amounts. Only very small amounts of the exogenous glucose are incorporated into the ethanol-precipitable carbohydrate fraction (which would include glycogen) by any of the preparations of eggs/larvae. However, the glucose is incorporated into higher molecular weight end-products that are liberated into the incubation medium. There is a temporal shift in the ability of activated larvae and oncospheres to metabolize exogenous glucose. Activated larvae and oncospheres absorb but do not metabolize glucose during the first 8 h post-activation. Between 8 and 16 h post-activation, however, virtually all of the absorbed glucose is metabolized into higher molecular weight end-products that are liberated into the incubation media. This temporal shift suggests that activation of oncospheres and cysticercoid morphogenesis are accompanied by distinct changes in carbohydrate metabolism.

Keywords

Hymenolepis diminutaoncospherecarbohydrate metabolismglucose metabolism.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 2 , February 1993 , pp. 201 - 209
Copyright
Copyright © Cambridge University Press 1993

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References

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