Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-26T00:56:42.107Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on the induction of permeability in Ascaris lumbricoides eggs

Published online by Cambridge University Press:  06 April 2009

J. Barrett
J. Barrett
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales
Get access Rights & Permissions [Opens in a new window]

Summary

The initial process in the hatching mechanism of Ascaris eggs is the sudden onset of permeability in the previously impermeable ascaroside membrane. During this change the ascaroside membrane remains intact and no chemical changes can be detected. Using the molecular probe 1-anilino-8-naphthalene sulphonic acid no conformational changes were detected in the ascaroside membrane during the induction of permeability. It is suggested that either the permeability change is due to a very localized chemical or conformational change, not detectable by conventional analytical techniques, or the change is due to mechanical damage of the ascaroside membrane, brought about by the activity of the infective larva.

Type
Research Article
Information
Parasitology , Volume 73 , Issue 1 , August 1976 , pp. 109 - 121
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barrett, J. (1968). Lipids of the infective and parasitic stages of some nematodes. Nature, London 218, 1267–8.CrossRefGoogle ScholarPubMed
Dodd, G. H. & Radda, G. K. (1969). 1-Anilinonaphthalene-8-sulphonate, a fluorescent conformational probe for glutamate dehydrogenase. Biochemical Journal 114, 407–17.CrossRefGoogle Scholar
Fairbairn, D. (1957). The biochemistry of Ascaris. Experimental Parasitology 6, 491–554.CrossRefGoogle ScholarPubMed
Fairbairn, D. (1961). The in vitro hatching of Ascaris lumbricoides eggs. Canadian Journal of Zoology 39, 153–62.CrossRefGoogle Scholar
Fairbairn, D. (1970). Biochemical adaptation and loss of genetic capacity in helminth parasites. Biological Reviews 45, 29–72.CrossRefGoogle ScholarPubMed
Foor, W. E. (1967). Ultrastructural aspects of oocyte development and shell formation in Ascaris lumbricoides. Journal of Parasitology 53, 1245–61.CrossRefGoogle ScholarPubMed
Freedman, R. B. & Radda, G. K. (1969). The interaction of 1-anilino-8-naphthalene sulphonate with erythrocyte membranes. FEBS Letters 3, 150–2.CrossRefGoogle ScholarPubMed
Jacin, H. & Mishkin, A. R. (1965). Separation of carbohydrates on borate-impregnated silica gel G plates. Journal of Chromatography 18, 170–3.CrossRefGoogle ScholarPubMed
Jacks, T. J. & Kircher, H. W. (1967). Fluorimetric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone. Analytical Biochemistry 21, 279–85.CrossRefGoogle Scholar
Jaskoski, B. J. & Colucci, A. V. (1964). In vitro hatching of Ascaris suum eggs. Transactions of the American Microscopical Society 83, 294–300.CrossRefGoogle Scholar
Jezyk, P. F. & Fairbairn, D. (1967). Ascarosides and ascaroside esters in Ascaris lumbricoides (Nematoda). Comparative Biochemistry and Physiology 23, 691–705.CrossRefGoogle ScholarPubMed
Justus, D. E. & Ivey, M. H. (1969). Chitinase activity in developmental stages of Ascaris swum and its inhibition by antibody. Journal of Parasitology 55, 472–6.CrossRefGoogle Scholar
Kaulenas, M. & Fairbairn, D. (1966). Induction of permeability in infective and uninfective eggs of Ascaris lumbricoides. Journal of Parasitology 52, 303–6.CrossRefGoogle Scholar
Kuby, S. A. & Lardy, H. A. (1953). Purification and kinetics of β-D-galactosidase from Escherichia coli, strain K12. Journal of the American Chemical Society 75, 890–6.CrossRefGoogle Scholar
Mead, J. A. R., Smith, J. N. & Williams, R. T. (1955). Studies in detoxication 67. The biosynthesis of the glucuronides of umbelliferone and 4-methylumbelliferone and their use in fiuorimetric determination of -glucuronidase. Biochemical Journal 61, 569–74.CrossRefGoogle Scholar
Mokrash, L. C. (1954). Analysis of hexose phosphates and sugar mixtures with the anthrone reagent. Journal of Biological Chemistry 208, 55–9.CrossRefGoogle Scholar
Morrison, W. R. & Smith, L. M. (1964). Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. Journal of Lipid Research 5, 600–8.CrossRefGoogle ScholarPubMed
Rogers, W. P. (1958). Physiology of the hatching of eggs of Ascaris lumbricoides. Nature, London 181, 1410–1.CrossRefGoogle Scholar
Tarr, G. E. & Fairbairn, D. (1973). Ascarosides of the ovaries and eggs of Ascaris lumbri-coides (Nematoda). Lipids 8, 7–16.CrossRefGoogle ScholarPubMed
Tomarelli, R. M., Charney, J. & Harding, M. L. (1949). The use of azoalbumin as a substrate in the colorimetric determination of peptic and tryptic activity. Journal of Laboratory and Clinical Medicine 34, 428–33.Google ScholarPubMed
Ward, C. W. & Fairbairn, D. (1970). Enzymes of β-oxidation and their function during development of Ascaris lumbricoides eggs. Developmental Biology 22, 366–87.CrossRefGoogle ScholarPubMed
Ward, K. A. & Fairbairn, D. (1972). Chitinase in developing eggs of Ascaris suum (Nematoda). Journal of Parasitology 58, 546–9.CrossRefGoogle ScholarPubMed