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Studies on muscle cells isolated from Schistosoma mansoni: a Ca2+-dependent K+ channel

Published online by Cambridge University Press:  06 April 2009

K. L. Blair ,
T. A. Day ,
M. C. Lewis ,
J. L. Bennett  and
R. A. Pax
K. L. Blair
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI, 48824, USA
T. A. Day
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI, 48824, USA
M. C. Lewis
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI, 48824, USA
J. L. Bennett
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI, 48824, USA
R. A. Pax
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University, East Lansing, MI, 48824, USA
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Summary

Muscle cells from adult male Schistosoma mansoni have been isolated using a combination of papain digestions and mechanical dissociation procedures. The muscle fibres isolated in this way lacked nuclei but they did contract and relax in response to high [K+], a response which was blocked in the presence of Co2+. From this we conclude that the isolation procedure yields viable muscle fibres useful for physiological studies. Patch-clamp recordings taken from the isolated fibres show a variety of discrete ionic conductances. In inside-out patches one prominent channel was a Ca2+-activated K+ channel with a conductance of 195 pS and a selectivity greater than 10:1 for K+ over Na+, Cs+ or NH4+. Percentage open time was dependent on [Ca2+] at the intracellular face. With [Ca2+] at 1 μM or greater percentage open time was > 95%; at 0.1 μM it was < 2%. No voltage sensitivity could be detected in the voltage range from –50 to –10 mV membrane potential. Ba2+ (10 mM), but neither tetraethylammonium nor 3,4-diaminopyridine blocked the channel from the intracellular face. This Ca2+-activated K+ channel in the muscle membrane of this acoelomate animal is similar in most respects to the maxi-K+ channels which have been described in a variety of cells from more highly evolved animals.

Keywords

Schistosoma mansonimuscleCa2+-activated K+ channelpatch-clamp
Type
Research Article
Information
Parasitology , Volume 102 , Issue 2 , April 1991 , pp. 251 - 258
Copyright
Copyright © Cambridge University Press 1991

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