Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-05T10:38:23.726Z Has data issue: false hasContentIssue false
  • English
  • Français

The specificity of purine base and nucleoside uptake in promastigotes of Leishmania braziliensis panamensis

Published online by Cambridge University Press:  06 April 2009

B. D. Hansen ,
J. Perez-Arbelo ,
J. F. Walkony  and
L. D. Hendricks
B. D. Hansen
Affiliation:
Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D.C. 20012
J. Perez-Arbelo
Affiliation:
Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D.C. 20012
J. F. Walkony
Affiliation:
Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D.C. 20012
L. D. Hendricks
Affiliation:
Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Washington, D.C. 20012
Get access Rights & Permissions [Opens in a new window]

Summary

Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported at a significantly slower rate than the purine nucleosides adenosine and inosine. Competitive interactions among those purines tested confirmed the presence of mediated and diffusion components and suggested that three transport loci may be operating (Fig. 6). The first transport locus, designated Locus 1, transported inosine, Locus 2, the purine bases hypoxanthine and adenine and Locus 3, adenosine. In addition, adenine and hypoxanthine inhibited the uptake of one another competitively. A comparison of Ki values derived from double reciprocal plots of labelled hypoxanthine and adenine uptake in the presence of the unlabelled substrates as inhibitors suggested that adenine has a greater affinity for the transport locus.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 2 , October 1982 , pp. 271 - 282
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Childs, G. E., Foster, K. A. & McRoberts, M. J. (1978). Insect cell culture media for cultivation of new world Leishmania. International Journal for Parasitology 8, 255–8.CrossRefGoogle ScholarPubMed
Fernandes, J. F. & Castellani, O. (1958). Nucleotide and polynucleotide synthesis in Trypanosoma cruzi, I. Precursors of purine compounds. Experimental Parasitology 7, 224–35.CrossRefGoogle ScholarPubMed
Gutteridge, W. E. & Coombs, G. H. (1977). Biochemistry of Parasitic Protozoa. Baltimore, London and Tokyo: University Park Press.CrossRefGoogle Scholar
Guttman, H. N. & Wallace, F. G. (1964). Nutrition and physiology of Trypanosomatidae. In Biochemistry and Physiology of Protozoa, vol. 3 (ed. Hutner, S. H.), pp. 459–94. New York and London.Google Scholar
Hansen, B. D. (1979). Trypanosoma gambiense: Membrane transport of amino acids. Experimental Parasitology 48, 296–304.CrossRefGoogle ScholarPubMed
Hendricks, L. D., Wood, D. E. & Hajduk, M. E. (1978). Haemoflagellates: commercially available liquid media for rapid cultivation. Parasitology 76, 309–16.CrossRefGoogle ScholarPubMed
Hendricks, L. & Wright, N. (1979). Diagnosis of cutaneous leishmaniasis by in vitro cultivation of saline aspirates in Schneider's Drosophila Medium. American Journal of Tropical Medicine and Hygiene 28, 962–4.CrossRefGoogle ScholarPubMed
Kidder, G. W., Dewey, V. C. & Nolan, L. L. (1977). Adenine deaminase from a eukaryotic animal cell, Crithidia fasiculata. Archives of Biochemistry and Biophysics 183, 7–12.CrossRefGoogle Scholar
Kilejian, A. (1966). Permeation of L-proline in the cestode, Hymenolepis diminuta. Journal of Parasitology 52, 1108–15.CrossRefGoogle ScholarPubMed
Konigk, E. (1978). Purine nucleotide metabolism in promastigotes of Leishmania tropica: Inhibitory effect of a allopurinol and analogues of purine nucleosides. Tropenmedizin und Parasitologie 29, 435–8.Google ScholarPubMed
Konigk, E. & Rasoul, S. A. (1978). Catabolism of adenosine 5-monophosphate in promastigotes of Leishmania tropica. Tropenmedizin und Parasitologie 29, 319–22.Google ScholarPubMed
Koszalka, G. W. & Krenitsky, T. A. (1979). Nucleosidases from Leishmania donovani. Journal of Biological Chemistry 254, 8185–93.CrossRefGoogle ScholarPubMed
Lineweaver, H. & Burke, D. (1934). The determination of enzyme dissociation constants. Journal of the American Chemical Society 56, 658–66.CrossRefGoogle Scholar
Mark, J. J., Berens, R. L. & Nelson, D. J. (1978). Purine metabolism in Leishmania donovani and Leishmania braziliensis. Biochemica et biophysica acta 544, 360–71.Google Scholar
Read, C. P., Rothman, A. H. & Simmons, J. E. Jr, (1963). Studies on membrane transport, with special reference to parasite—host integration. Annals of the New York Academy of Sciences 133, 154–205.CrossRefGoogle Scholar
Ruff, M. D. & Read, C. P. (1974). Specificity of carbohydrate transport in Trypanosoma equiperdum. Parasitology 68, 103–15.CrossRefGoogle ScholarPubMed
Schneider, I. (1974). Formulations. GIBCO catalogue, Grand Island Biological Company, Grand Island, New York p. 144.Google Scholar
Spector, T., Jones, T. E. & Elion, G. B. (1979). Specificity of adenylosuccinate synthetase and adenylsuccinate lyase from Leishmania donovani. Journal of Biological Chemistry 254, 8422–6.CrossRefGoogle Scholar
Steiger, R. F. & Steiger, E. (1977). Cultivation of Leishmania donovani and Leishmania braziliensis in defined media: nutritional requirements. Journal of Protozoology 24, 437–41.CrossRefGoogle ScholarPubMed
Tuttle, J. V. & Krenitsky, T. A. (1979). Purine phosphoribosyltransferases from Leishmania donovani. Journal of Biological Chemistry 255, 909–16.CrossRefGoogle Scholar
Webster, H. K. & Whaun, J. M. (1981). Application of simultaneous UV-radioactivity high performance liquid chromatography to the study of intermediary metabolism: I. purine nucleotides, nucleosides and bases. Journal of Chromatography 209, 283–92.CrossRefGoogle Scholar
Yoneda, S. (1971). Some aspects of the purine metabolism of Trypanosoma cruzi in tissue culture. Revista Brasilera de Pesquisas Medicina y Biologia 4, 205–18.Google ScholarPubMed