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Specific tyrosine phosphorylation induced in Schistosoma mansoni miracidia by haemolymph from schistosome susceptible, but not resistant, Biomphalaria glabrata

Published online by Cambridge University Press:  06 December 2007

A. J. WALKER  and
D. ROLLINSON
A. J. WALKER*
Affiliation:
School of Life Sciences, Kingston University, Penrhyn Road, Kingston upon Thames, Surrey KT1 2EE, UK
D. ROLLINSON
Affiliation:
Wolfson Wellcome Biomedical Laboratories, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
*
*Corresponding author: School of Life Sciences, Kingston University, Kingston upon Thames, Surrey KT1 2EE, UK. Tel: +44 20 8547 2000. Fax: +44 20 8547 7497. E-mail: [email protected]
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Summary

Molecular interplay during snail-schistosome interactions is poorly understood and there is much to discover concerning the effect of snail host molecules on molecular processes in schistosomes. Using the Biomphalaria glabrata – Schistosoma mansoni host-parasite system, the effects of exposure to haemolymph, derived from schistosome-resistant and susceptible snail strains, on protein tyrosine phosphorylation in miracidia have been investigated. Western blotting revealed several tyrosine phosphorylated proteins in this larval stage. Exposure of miracidia to haemolymph from susceptible snails for 60 min resulted in a striking, 5-fold, increase in the tyrosine phosphorylation of a 56 kDa (p56) S. mansoni protein. In contrast, haemolymph from resistant snails had little effect on protein tyrosine phosphorylation levels in miracidia. Confocal microscopy revealed that tyrosine phosphorylation was predominantly associated with proteins present in the tegument. Finally, treatment of miracidia with the tyrosine kinase inhibitor genistein significantly impaired their development into primary sporocysts. The results open avenues for research that focus on the potential importance of phospho-p56 to the outcome of schistosome infection in snails, and the significance of protein tyrosine kinase-mediated signalling events to the transformation of S. mansoni larvae.

Keywords

Schistosoma mansoniBiomphalaria glabratamiracidiatyrosine kinaseprotein phosphorylationmolluscan defencehaemolymphhost-parasite interactions
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 3 , March 2008 , pp. 337 - 345
Copyright
Copyright © Cambridge University Press 2007

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