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The specific immunoglobulin response in cattle immunized with Theileria parva (Muguga) stabilate

Published online by Cambridge University Press:  06 April 2009

W. P. H. Duffus  and
G. G. Wagner
W. P. H. Duffus
Affiliation:
Veterinary Research Laboratories, P.O. Kabete, Kenya
G. G. Wagner
Affiliation:
East African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya
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Extract

Immunoglobulin synthesis in response to experimental infection of cattle with Theileria parva (Muguga) was studied by Sephadex G 200 chromatography. Serum samples were collected for 88 days following inoculation. Indirect fluorescent antibody (IFA), complement fixation (CF), indirect haemagglutination (IHA) and immunodiffusion (ID) tests were used to detect activity against T. parva piroplasm antigen. There was a small and transient IgM response in the IFA test, thereafter all IFA activity was 7S Ig. A sequential production of IgM and 7S Ig CF antibodies was demonstrated. In all sera tested only IgM activity was demonstrated by the IHA test. These results are compared with those from similar studies with T. parva and other protozoan diseases, especially in the differential immunoglobulin response shown by the contrasting results from the three serological tests.

Type
Research Article
Information
Parasitology , Volume 69 , Issue 1 , August 1974 , pp. 31 - 41
Copyright
Copyright © Cambridge University Press 1974

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