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The source and nature of some functional antigens of Trichuris muris

Published online by Cambridge University Press:  06 April 2009

Simon N. Jenkins  and
D. Wakelin
Simon N. Jenkins
Affiliation:
Wllcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Bearsden, Glasgow, G61 1QH
D. Wakelin
Affiliation:
Wllcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Bearsden, Glasgow, G61 1QH
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Extract

Vaccination with whole male worm extract (AMA), stichoseome extract (SA) and a short-term inculbation fluid (EXA) of adult Trichuris muris induced a high degree of protective immunity in mice as assessed by reduction in larval worm burden.

Immunodiffusion of AMA against rabbit anti-AMA serum revealed five precipitin lines, one of which was common to both EXA and SA with which only single lines were obtained.

Physico-chemical tests suggested that the antigen is a protein and that carbohydrate, lipid and DNA do not contribute to its antigenicity. Partial fractionation was obtained with ammonium sulphate. All preparations with immunogenic activity showed the common line of EXA on immunodiffusion.

It is concluded that one of the protective immunogens is a protein which can be associated with the precipitin line and originates in the stichosome.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 2 , April 1977 , pp. 153 - 161
Copyright
Copyright © Cambridge University Press 1977

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References

Birnie, G. D., Delcour, J., Angus, D., Threlfall, G. & Paul, J. (1974). Distribution of nuclear RNA's in growing and nongrowing cells. Cold Spring Harbor Conferences on Cell Proliferation, Vol. 1 (ed. Clarkson, B. and Baserga, R.), pp. 757–68.Google Scholar
Cypess, R. (1970). Artificial production of acquired immunity in mice by footpad injections of crude larval extract of Nematospiroides dubius. Journal of Parasitology 56, 320.CrossRefGoogle ScholarPubMed
Dawson, R. M. C., Elliott, D. C., Elliott, W. H. & Jones, K. M. (1969). Data for Biochemical Research. Oxford University Press.Google Scholar
Despommier, D. D. & Muller, M. (1969). Particle associated functional antigens of Trichinella spiralis larvae and immunity in mice. Wiadomosci Parazytologiczne 15, 612.Google Scholar
Despommier, D. D. & Muller, M. (1970). The stichosome of Trichinella spiralis:its structure and function. Journal of Parasitology 56, section II, part I, p. 76.Google Scholar
Guerrero, J. & Silverman, P. H. (1969). Ascaris suum. Immune reactions in mice. 1. Larval metabolic and somatic antigens. Experimental Parasitology 26, 272–81.CrossRefGoogle Scholar
Jenkins, S. N. (1976). Studies on the functional antigens of Trichuris muris. Parasitology 73, 14.Google Scholar
Jenkins, T. (1970). A morphological and histochemical study of Trichuris suis (Schrank 1788) with special reference to host parasite relationship. Parasitology 61, 357–74.CrossRefGoogle Scholar
Larsh, J. E. (1963). Experimental Trichiniasis, Advances in Parasitology 1, 213–82.CrossRefGoogle Scholar
Larsh, J. E., Goulson, H. T., Weatherly, N. F. & Chaffee, E. F. (1970). Studies on delayed (cellular) hypersensitivity in mice infected with Trichinella spiralis, V. Tests in recipients injected with donor spleen cells 1, 3, 7, 14 or 20 days before infection. Journal of Parasitology 56, 978–81.CrossRefGoogle Scholar
Nimmo-Smtth, R. H. & Keeling, J. E. D. (1960). Some hydrolytic enzymes of the parasitic nematode Trichuris muris. Experimental Parasitology 10, 337–55.CrossRefGoogle Scholar
Rothwell, T. L. W. & Love, R. J. (1974). Vaccination against the nematode Trichostrongylus colubriformis. I. Vaccination of guinea pigs with worm homogenates and soluble products released during in vitro maintenance. International Journal for Parasitology 4, 293–9.CrossRefGoogle ScholarPubMed
Sokal, R. R. & Rohlf, F. J. (1969). Biometry. San Francisco: W. H. Freeman and Co.Google Scholar
Soulsby, E. J. L. (1963). The nature and origin of the functional antigens in helminth infections. Annals of the New York Academy of Sciences 113, 492–509.CrossRefGoogle Scholar
Van Zandt, P. D. (1971). Immunity relationships in white mice infected with Nemato-spiroides dubius. III. Artificial immunization with antigen prepared from adults. Journal of the Elisha Mitchell Scientific Society 87, 74–6.Google Scholar
Waddell, W. J. (1956). A simple ultraviolet spectrophotometric method for the determination of protein. Journal of Laboratory and Clinical Medicine 48, 311–4.Google ScholarPubMed
Wakelin, D. (1967). Acquired immunity to Trichuris muris in the albino laboratory mouse. Parasitology 57, 515–24.CrossRefGoogle ScholarPubMed
Wakelin, D. & Selby, G. R. (1973). Functional antigens of Trichuris muris. The stimulation of immunity by vaccination of muce with somatic antigen preparations. International Journal for Parasitology 3, 711–15.CrossRefGoogle Scholar