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Small subunit (18S) ribosomal RNA gene divergence in the genus Schistosoma

Published online by Cambridge University Press:  06 April 2009

D. A. Johnston ,
R. A. Kane  and
D. Rollinson
D. A. Johnston
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
R. A. Kane
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
D. Rollinson
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
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Summary

An entire 18S rRNA gene sequence from Schistosoma spindale (1990 bases) and partial 18S rRNA gene sequences from S. haematobium (1950 bases) and S. japonicum (1648 bases) have been determined. Together with the previously published sequence for the S. mansoni 18S rRNA gene, these data encompass the 4 recognized Schistosoma species groups. Although Schistosoma 18S rRNA genes are highly conserved, the sequences permit a preliminary molecular phylogeny to be established for the genus. This identifies S. haematobium and S. spindale as sister taxa in a clade with S. mansoni. S. japonicum does not appear to be closely related to this clade. Much of the observed variation occurs within a ‘hypervariable’ stretch of the gene corresponding to part of the V4 region of 18S rRNA. Despite this variation, the 3 new sequences fit models of 18S rRNA secondary structure predicted from the S. mansoni sequence.

The sequences reported in this paper have been submitted to the EMBL Database under accession numbers Z11976 (S. haematobium), Z11590 (S. japonicum) and Z11979 (S. spindale).

Keywords

Schistosomasequenceribosomal RNA18Sphylogenysecondary structure.
Type
Research Article
Information
Parasitology , Volume 107 , Issue 2 , August 1993 , pp. 147 - 156
Copyright
Copyright © Cambridge University Press 1993

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