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Single nucleotide polymorphism (SNP) markers for benzimidazole resistance in veterinary nematodes

Published online by Cambridge University Press:  03 July 2007

G. VON SAMSON-HIMMELSTJERNA ,
W. J. BLACKHALL ,
J. S. McCARTHY  and
P. J. SKUCE
G. VON SAMSON-HIMMELSTJERNA*
Affiliation:
Institute for Parasitology, University of Veterinary Medicine Foundation, Bünteweg 17, Hannover 30559, Germany
W. J. BLACKHALL
Affiliation:
Institute for Parasitology, University of Veterinary Medicine Foundation, Bünteweg 17, Hannover 30559, Germany
J. S. McCARTHY
Affiliation:
Queensland Institute of Medical Research, University of Queensland, Herston QLD 4006, Australia
P. J. SKUCE
Affiliation:
Moredun Research Institute, Pentlands Science Park, Edinburgh EH26 0PZ, UK
*
*Corresponding author: Institute for Parasitology, University of Veterinary Medicine Foundation, Bünteweg 17, Hannover 30559, Germany. Tel: +49 511 953 8557. Fax: +49 511 953 8555. E-mail: [email protected]
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Summary

Resistance to the benzimidazole class of anthelmintics in nematodes of veterinary importance has a long history. Research into the mechanisms responsible for this resistance is subsequently at a more advanced stage than for other classes of anthelmintics. The principal mechanism of resistance to benzimidazoles is likely to involve changes in the primary structure of β-tubulins, the building blocks of microtubules. Specifically, point mutations in the β-tubulin isotype 1 gene leading to amino acid substitutions in codons 167, 198, and 200 of the protein have been associated with resistance in nematodes. These single nucleotide polymorphisms offer a means of detecting the presence of resistance within populations. In this mini-review, we focus on the prevalence and importance of these polymorphisms in three groups of nematodes: trichostrongylids, cyathostomins, and hookworms. A brief overview of existing strategies for genotyping single nucleotide polymorphisms is also presented. The CARS initiative hopes to exploit these known polymorphisms to further our understanding of the phenomenon of BZ resistance.

Keywords

BenzimidazoleresistanceSNPtrichostrongylidcyathostominhookworm
Type
Research Article
Information
Parasitology , Volume 134 , Special Issue 8: MARKERS FOR ANTHELMINTIC RESISTANCE , August 2007 , pp. 1077 - 1086
Copyright
Copyright © Cambridge University Press 2007

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