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Serotonin and its requirement for maintenance of contractility in muscle fibres isolated from Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

T. A. Day ,
J. L. Bennett  and
R. A. Pax
T. A. Day
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48814, USA
J. L. Bennett
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48814, USA
R. A. Pax
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48814, USA
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Summary

Muscle fibres isolated from adult Schistosoma mansoni contracted in a dose-dependent manner when exposed to elevated K+ with a maximum response obtained with 25 mM K+. These contractions were dependent on extracellular Ca2+ since Co2+ (5 mM) or nicardipine (1 μM) blocked the high K+ contractions. Serotonin (300 nM or higher) was required for maintenance of high K+ contractions. With concentrations of serotonin less than 300 nM the response was dose dependent. 5-Methoxytryptamine or α-methylserotonin at 1 μM as well as 10 μM tryptamine were able to substitute for serotonin, but 1 μM 5-carboxyamidotryptamine was ineffective. The order of potency for antagonists (10 μM) was: methiothepin > metergoline > Ly-278,584 = ketanserin. This pattern of responsiveness does not fit well with any known mammalian serotonin receptor subtype. Since forskolin, an adenylate cyclase activator, is able to mimic the action of serotonin and H89, a protein kinase inhibitor, is able to block the effect of serotonin, the effect of serotonin on contractility of the muscle may be via a cAMP-dependent pathway.

Keywords

Schistosoma mansoniplatyhelminthtrematodemuscleserotonin receptorcAMP
Type
Research Article
Information
Parasitology , Volume 108 , Issue 4 , May 1994 , pp. 425 - 432
Copyright
Copyright © Cambridge University Press 1994

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References

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