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Schistosoma mansoni TOR is a tetraspanning orphan receptor on the parasite surface

Published online by Cambridge University Press:  13 March 2009

C. LOCHMATTER ,
J. A. SCHIFFERLI  and
P. J. MARTIN
C. LOCHMATTER*
Affiliation:
Department of Biomedicine, Immunonephrology, University Hospital Basel, Hebelstrasse 20, CH-4031 Basel, Switzerland
J. A. SCHIFFERLI
Affiliation:
Department of Biomedicine, Immunonephrology, University Hospital Basel, Hebelstrasse 20, CH-4031 Basel, Switzerland
P. J. MARTIN
Affiliation:
Department of Biomedicine, Immunonephrology, University Hospital Basel, Hebelstrasse 20, CH-4031 Basel, Switzerland
*
*Corresponding author. Tel: +41 61 2653891. Fax: +41 61 2652350. E-mail: [email protected]
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Summary

A trispanning orphan receptor (TOR) has been described in Schistosoma haematobium and S. mansoni. Here we report the complete molecular organization of the S. mansoni TOR gene, also known as SmCRIT (complement C2 receptor inhibitor trispanning). The SmTOR gene consists of 4 exons and 3 introns as shown by cloning the single exons from S. mansoni genomic DNA and the corresponding cDNA from the larval stage (cercaria) and the adult worm. The SmTOR ORF consists of 1260 bp and is longer than previously reported, with a fourth trans-membrane domain (proposed new name: Tetraspanning Orphan Receptor) and with, however, an unchanged C2-binding domain on the extracellular domain 1 (ed1). This domain differs in S. japonicum. A protein at the approximate expected molecular weight (55 kDa) was detected in adult worm extracts with polyclonal and monoclonal antibodies, and was found to be expressed on the tegumental surface of cercariae.

Keywords

CRITTORSchistosomacomplement regulation
Type
Research Article
Information
Parasitology , Volume 136 , Issue 5 , April 2009 , pp. 487 - 498
Copyright
Copyright © 2009 Cambridge University Press

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