Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-22T23:35:09.207Z Has data issue: false hasContentIssue false
  • English
  • Français

Schistosoma mansoni: scanning electron microscopy of schistosomula, adults and eggs grown in vitro

Published online by Cambridge University Press:  06 April 2009

P. F. Basch  and
Natalicia Basch
P. F. Basch
Affiliation:
Department of Family, Community and Preventive Medicine, Stanford University School of Medicine, Stanford, California 94305
Natalicia Basch
Affiliation:
Department of Family, Community and Preventive Medicine, Stanford University School of Medicine, Stanford, California 94305
Get access Rights & Permissions [Opens in a new window]

Summary

Development of surface topography of cultured Schistosoma mansoni was followed in the cercariae, newly transformed and older schistosomula, and adults. Comparison with surface features of schistosomes from animal infections revealed no apparent differences in tegumental folding or morphogenesis, spines, sensory structures, or other components. Eggs produced by worm pairs grown in vitro were grossly abnormal, usually composed of loosely packed vitelline granules and lacking a shell.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 2 , October 1982 , pp. 333 - 338
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Basch, P. F. (1981 a). Cultivation of Schistosoma mansoni in vitro. I. Establishment of cultures from cercariae and development until pairing. Journal of Parasitology 67, 179–85.CrossRefGoogle ScholarPubMed
Basch, P. F. (1981 b). Cultivation of Schistosoma mansoni in vitro. II. Production of infertile eggs by worm pairs cultured from cercariae. Journal of Parasitology 67, 186–90.CrossRefGoogle ScholarPubMed
Crabtree, J. E. & Wilson, R. A. (1980). Schistosoma mansoni: a scanning electron microscope study of the developing schistosomulum. Parasitology 81, 553–64.CrossRefGoogle ScholarPubMed
Hockley, D. J. (1973). Ultrastructure of the tegument of Schistosoma. In Advances in Parasitology, vol. 11 (ed. B., Dawes), pp. 233–305. London: Academic Press.Google Scholar
Hockley, D. J. & Mclaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm. International Journal for Parasitology 3, 13–25.CrossRefGoogle ScholarPubMed
McLaren, D. J. (1980). Schistosoma mansoni: The Parasite Surface in Relation to Immunity. Letchworth, Herts: Research Studies Press Division of John Wiley & Sons, Ltd.Google Scholar
Price, Z., Voge, M. & Beydler, S. (1978). Changes in the tegumental surface of Schistosoma mansoni during development in the mammalian host. Scanning Electron Microscopy/1978 2, 399–403.Google Scholar
Race, G. J., Martin, J. H., Moore, D. V. & Larch, J. E. Jr. (1971). Scanning and transmission electronmicroscopy of Schistosoma mansoni eggs, cercariae and adults. The American Journal of Tropical Medicine and Hygiene 20, 914–24.CrossRefGoogle ScholarPubMed
Sakamoto, K. & Ishii, Y. (1976). Fine structure of schistosome eggs as seen through the scanning electron microscope. The American Journal of Tropical Medicine and Hygiene 25, 841–4.CrossRefGoogle ScholarPubMed
Samuelson, J. C., Caulfield, J. P. & David, J. R. (1980). Schistosoma mansoni: post-transformational surface changes in schistosomula grown in vitro and in mice. Experimental Parasitology 50, 369–83.CrossRefGoogle ScholarPubMed
Senft, A. W. & Gibler, W. B. (1977). Schistosoma mansoni tegumental appendages: scanning microscopy following thiocarbohydrazide-osmium preparation. The American Journal of Tropical Medicine and Hygiene 26, 1169–77.CrossRefGoogle ScholarPubMed
Senft, A. W., Gibler, W. B. & Knopf, P. M. (1978). Scanning electron microscope observations on tegument maturation in Schistosoma mansoni grown in permissive and non-permissive hosts. The American Journal of Tropical Medicine and Hygiene 27, 258–66.CrossRefGoogle ScholarPubMed
Silk, M. H., Spence, I. M. & Buch, B. (1970). Observations on Schistosoma mansoni blood flukes in the scanning electron microscope. South African Journal of Medical Science 35, 23–9.Google ScholarPubMed
Voge, M., Price, Z. & Bruckner, D. A. (1978). Changes in tegumental surface during development of Schistosoma mansoni. Journal of Parasitology 64, 585–92.CrossRefGoogle ScholarPubMed