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Schistosoma mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse

Published online by Cambridge University Press:  06 April 2009

Avis J. Mastin ,
Q. D. Bickle  and
R. A. Wilson
Avis J. Mastin
Affiliation:
Department of Biology, University of York, Y01 5DD
Q. D. Bickle
Affiliation:
Winches Farm Field Station, 395 Hatfield Road, St Albans, Herts AL4 OXQ
R. A. Wilson
Affiliation:
Department of Biology, University of York, Y01 5DD
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Summary

The fate of irradiated, immunizing cercariae and challenge schistosomula was investigated in mice using a quantitative, histological technique which would appear to be more efficient in estimating parasite numbers in skin and lungs than does the tissue mincing and incubation recovery technique used previously by other workers. There was evidence for slight retardation of irradiated (20 krad.) parasite migration in skin, but death of schistosomula in the skin appeared negligible. It was observed that the majority of irradiated parasites remained in the lungs until at least day 21 after infection, and that most schistosomula observed at this time were dead. In mice immunized with irradiated cercariae there was no evidence of attrition of the challenge infection in the skin. The migration profile of challenge parasites in the lungs of immunized animals was not significantly different from that of normal parasites in the lungs of naive animals. Dead challenge parasites were observed in the lungs but their numbers were not considered great enough to account for the final attrition measured by perfusion. It is suggested that the remainder of the attrition of challenge schistosomula may occur in the systemic and/or hepatic portal circulation.

Type
Research Article
Information
Parasitology , Volume 87 , Issue 1 , August 1983 , pp. 87 - 102
Copyright
Copyright © Cambridge University Press 1983

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