Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-19T04:34:50.020Z Has data issue: false hasContentIssue false
  • English
  • Français

Schistosoma mansoni: evidence that ‘non-permissiveness’ in 129/Ola mice involves worm relocation and attrition in the lungs

Published online by Cambridge University Press:  06 April 2009

A. A. F. Elsaghier ,
P. M. Knopf ,
G. F. Mitchell  and
D. J. Mclaren
A. A. F. Elsaghier
Affiliation:
National Institute for Medical Research, Mill Hill, London NW7 1A A, UK
P. M. Knopf
Affiliation:
Brown University, Rhode Island, USA
G. F. Mitchell
Affiliation:
Walter & Eliza Hall Institute of Medical Research, Melbourne, Australia
D. J. Mclaren
Affiliation:
National Institute for Medical Research, Mill Hill, London NW7 1A A, UK
Get access Rights & Permissions [Opens in a new window]

Summary

129/Ola mice resemble WEHI 129J mice in that around 70% of the individuals in any given population resist a primary infection with Schistosoma mansoni. Squashed-organ autoradiographic tracking of [Se]selenomethionine-labelled parasites has shown that the kinetics of worm migration in 129/Ola mice follows the expected pattern, and that all rodents harbour essentially similar numbers of worms on day 14 post-infection. Combined lung and liver worm recovery techniques have revealed, however, that segregation of mice into ‘permissive’ and ‘non-permissive’ individuals can first be detected on day 20. ‘Non-permissive’ mice are characterized by the absence of schistosome eggs at all times in the liver parenchyma and, in consequence, lack the attendant manifestations of pathology; they do, however, harbour a few stunted worms in the liver and significant numbers of adult schistosomes in the pulmonary vasculature. Histological analysis of sectioned lung tissue from such animals indicates that some lung-located schistosomes feed, pair and lay eggs. Nevertheless, eosinophil-enriched inflammatory reactions develop around such worms and the parasites themselves exhibit various manifestations of trauma, ranging from minor vacuolation to gut herniation and extrusion. The phenomenon of ‘non-permissiveness’ thus involves retardation of worm development in the liver and, in consequence, relocation of the parasites to the lungs, where they become subject to host effector responses.

Keywords

Schistosoma mansoni129/Ola micenon-permissivenessautoradiographic trackingrelocationlung inflammation.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 3 , December 1989 , pp. 365 - 375
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Auriault, C., Balloul, J. M., Pierce, R. J., Damonneville, M., Sondermeyer, P. & Capron, A. (1987). Helper T cells induced by a purified 28-kilodalton antigen of Schistosoma mansoni protect rats against infection. Infection and Immunity 55, 1163–9.CrossRefGoogle ScholarPubMed
Christensen, N. O. (1977). A method for the in vitro labelling of Schistosoma mansoni and S. intercalatum cercariae with radioselenium. Zeitschrift für Parasitenkunde 54, 275–88.CrossRefGoogle Scholar
Davern, K. M., Tiu, W. U., Wright, M. D., Garcia, E. G. & Mitchell, G. F. (1987). Responses in mice to Sj26, a glutathione S-transferase of Schistosoma japonicum worms. Immunology and Cell Biology 65, 473–82.CrossRefGoogle ScholarPubMed
Garcia, E. G., Tiu, W. U. & Mitchell, G. F. (1983). Innate resistance to Schistosoma japonicum in a proportion of 129/J mice. Journal of Parasitology 69, 613–15.CrossRefGoogle Scholar
Holy, J. M., O'Leary, K. A., Oaks, J. A. & Tracy, J. W. (1989). Immunocytochemical localization of the major glutathione S-transferases in adult Schistosoma mansoni. (in the press).CrossRefGoogle Scholar
Kamiya, H. & Mclaren, D. J. (1987). Sckistosoma mansoni: migration potential of normal and radiation attenuated parasites in naive guinea pigs. Experimental Parasitology 63, 98–107.CrossRefGoogle Scholar
Kamiya, H., Smithers, S. R. & Mclaren, D. J. (1987). Schistosoma mansoni: autoradiographic tracking studies of isotopically labelled challenge parasites in naive and vaccinated CBA/Ca mice. Parasite Immunology 9, 515–29.CrossRefGoogle ScholarPubMed
Mangold, B. L. & Dean, D. A. (1983). Autoradiographic analysis of Schistosoma mansoni migration from skin to lungs in naive mice. Evidence that most attrition occurs after the skin phase. American Journal of Tropical Medicine and Hygiene 32, 785–9.CrossRefGoogle ScholarPubMed
Mannervik, B. (1985). The isoenzymes of glutathione transferase. Advances in Enzymology 57, 357–417.Google ScholarPubMed
Mclaren, D. J. & Terry, R. J. (1982). The protective role of acquired host antigens during schistosome maturation. Parasite Immunology 4, 129–48.CrossRefGoogle ScholarPubMed
Mitchell, G. F., Cruise, K. M., Garcia, E. G. & Tiu, W. U. (1984). Anti-worm antibody specificities in 129/J mice resistant to infection with Schistosoma japonicum. Journal of Parasitology 70, 983–5.CrossRefGoogle ScholarPubMed
Smith, D. B., Davern, K. M., Board, P. G., Tiu, W. U., Garcia, E. G. & Mitchell, G. F. (1986). Mr 26000 antigen of Schistosoma japonicum recognised by resistant WEHI 129/J mice is a parasite glutathione S-transferase. Proceedings of the National Academy of Sciences, USA 83, 8703–7.CrossRefGoogle Scholar
Smithers, S. R. & Gammage, K. (1980). Recovery of Schistosoma mansoni from the skin, lungs and hepatic portal system of naive mice and mice previously exposed to S. mansoni: evidence for two phases of parasite attrition in immune mice. Parasitology 80, 289–300.CrossRefGoogle Scholar
Smithers, S. R. & Terry, R. J. (1965). Infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Taylor, J. B., Vidal, A., Torpier, G., Meyer, D. J., Roitsch, C., Balloul, J. M., Southaw, C., Sondermeyer, P., Pemble, S., Lecocq, J. P., Capron, A. & Ketterer, B. (1988). The glutathione transferase activity and tissue distribution of a cloned Mr 28K protective antigen of Schistosoma mansoni.EMBO Journal 3, 465–72.CrossRefGoogle Scholar
Tiu, W. U., Ehl, A., Walker, J. C., Garcia, E. G., Smith, D. B. & Mitchell, G. F. (1986). Resistance of 129/J mice to Schistosoma mansoni infection. Australian Journal of Experimental Biology and Medical Science 64, 345–9.CrossRefGoogle ScholarPubMed
Wilson, R. A. (1987). Cercariae to liver worms: development and migration in the mammalian host. In The Biology of Schistosomes: From Genes to Latrines (ed. Rollinson, D. & Simpson, A.), London: Academic Press. pp. 115–46.Google Scholar
Wilson, R. A. & Coulson, P. S. (1984). The effect of softwood sawdust bedding on the maturation of an infection of Schistosoma mansoni in mice exposed to cercariae via the tail or abdominal skin. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 411–12.CrossRefGoogle ScholarPubMed