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Schistosoma mansoni: changes in elemental composition in relation to the age and sexual status of the worms

Published online by Cambridge University Press:  06 April 2009

M. K. Shaw  and
D. A. Erasmus
M. K. Shaw
Affiliation:
Department of Zoology, University College, Cardiff
D. A. Erasmus
Affiliation:
Department of Zoology, University College, Cardiff
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Summary

Changes during development in the elemental contents (Ca, P, K, Mg and S) of male and female Schistosoma mansoni from both mixed and single sex infections have been measured for the first time. In mixed infections, there is a significant difference between the calcium content of male and female worms; female worms having between 2 and 3 times more calcium. In mature female worms from mixed infections, after an initial rise in the calcium content corresponding to the time when vitelline development occurs, the amount of calcium ranged from 0.36 to 0.65 mg/g dry wt, whereas the amounts in similarly aged male worms ranged between 0.19 and 0.32 mg/g dry wt. While the type of infection does not affect the calcium content of male worms, a significant difference in the calcium levels in female worms from mixed and single sex infections was found. The amount of calcium in females from single sex infections ranged from 0.21 to 0.39 mg/g dry wt and this is considerably less than the levels recorded in females from mixed infections. This difference in calcium content between mixed and single sex infections can be related to the differences in degree of development of the vitelline gland in the two types of female worm. The amounts of phosphorus, potassium, magnesium and sulphur in both male and female worms from both single and mixed infections are similar, although females from mixed infections contained slightly more phosphorus than either females from single sex infections or either type of male worm.

Type
Research Article
Information
Parasitology , Volume 86 , Issue 3 , June 1983 , pp. 439 - 453
Copyright
Copyright © Cambridge University Press 1983

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References

REFERENCES

Agnes, F., Bestetti, G. & Falagiani, P. (1972). The mineral element content of Fasciola hepatica in a parasitic environment and after survival in vitro. Folia veterinaria Latina 2, 326–45.Google Scholar
Barrett, J. (1981). Biochemistry of Parasitic Helminths. London: Macmillan Publishers Ltd.CrossRefGoogle Scholar
von Brand, T. (1966). Biochemistry of Parasites. New York: Academic Press.Google Scholar
von Brand, T. (1979). Biochemistry and Physiology of Endoparasites. Amsterdam: Elsevier/North Holland Biomedical Press.Google Scholar
Cesari, I. M., Simpson, A. J. G. & Evans, W. H. (1981). Properties of a series of tegumental membrane-bound phosphohydrolase activities of Schistosoma mansoni. The Biochemical Journal 198, 467–73.CrossRefGoogle ScholarPubMed
Coles, G. C. (1973). The metabolism of schistosomes: A review. International Journal of Biochemistry 4, 319–37.CrossRefGoogle Scholar
Coles, G. C. (1975). Fluke biochemistry – Fasciola and Schistosoma. Helminthological Abstracts 44, 147–62.Google Scholar
Davies, T. W. & Morgan, A. J. (1976). The application of X-ray analysis in the transmission electron analytical microscope (T.E.A.M.) to the quantitative bulk analysis of biological microsamples. Journal of Microscopy 107, 47–54.CrossRefGoogle Scholar
Dorsey, C. H. & Stirewalt, M. A. (1977). Schistosoma mansoni: localisation of calcium-detecting reagents in electron-lucent areas of specific preacetabular gland granules. Zeitschrift für Parasitenkunde 54, 165–73.CrossRefGoogle ScholarPubMed
Dresden, M. H. & Edlin, E. M. (1975). Schistosoma mansoni: calcium content of cercariae and its effects on protease activity in vitro. Journal of Parasitology 61, 398–402.CrossRefGoogle Scholar
Duvall, R. H. & Dewitt, W. B. (1967). An improved perfusion technique for recovering adult schistosomes from laboratory animals. American Journal of Tropical Medicine and Hygiene 16, 483–6.CrossRefGoogle ScholarPubMed
Erasmus, D. A. (1972). The Biology of Trematodes. London: Edward Arnold.Google Scholar
Erasmus, D. A. (1973). A comparative study of the reproductive system of mature, immature and ‘unisexual’ female Schistosoma mansoni. Parasitology 67, 165–83.CrossRefGoogle ScholarPubMed
Erasmus, D. A. & Davies, T. W. (1979). Schistosoma mansoni and S. haematobium: Calcium metabolism of the vitelline cell. Experimental Parasitology 47, 91–106.CrossRefGoogle ScholarPubMed
Erasmus, D. A., Popiel, I. & Shaw, J. R. (1982). A comparative study of the vitelline cell in Schistosoma mansoni, S. haematobium, S. japonicum and S. mattheei. Parasitology 84, 283–7.CrossRefGoogle ScholarPubMed
Fetterer, R. H., Pax, R. A. & Bennett, J. L. (1980 a). Praziquantel, potassium and 2,4 dinitrophenol: analysis of their action on the musculature of Schistosoma mansoni. European Journal of Pharmacology 64, 31–8.CrossRefGoogle ScholarPubMed
Fetterer, R. H., Pax, R. A. & Bennett, J. L. (1980 b). Schistosoma mansoni: characterization of the electrical potential from the tegument of adult males. Experimental Parasitology 49, 353–65.CrossRefGoogle ScholarPubMed
Gaur, A. S. & Agarwal, S. M. (1980). Biochemical studies on Paramphistomum cervi (Schrank, 1790) (Trematoda: Paramphistomatidae). Indian Journal oj Experimental Biology 18, 1518–19.Google Scholar
Goil, M. M. (1964). Physiological studies on trematodes: calcium, magnesium and phosphorus contents. Parasitology 54, 567–70.CrossRefGoogle Scholar
Gönnert, R. (1955). Schistosomiasis studies II. Über die Eibildung der Schistosoma mansoni und das Schicksal der Eier im Wirtsorganismus. Zeitschrift für Tropenmedizin und Parasitologie 6, 33–52.Google Scholar
Halton, D. W., Stranock, S. D. & Hardcastle, A. (1974). Vitelline cell development in monogenean parasites. Zeitschrift für Parasitenkunde 45, 45–61.CrossRefGoogle ScholarPubMed
Irwin, S. W. B. & Threadgold, L. T. (1970). Electron microscope studies on Fasciola hepatica. VIII. The development of the vitelline cells. Experimental Parasitology 28, 399–411.CrossRefGoogle ScholarPubMed
Irwin, S. W. B. & Maguire, J. G. (1979). Ultrastructure of the vitelline follicles of Gorgoderina vitelliloba (Trematoda: Gorgoderidae). International Journal for Parasitology 9, 47–53.CrossRefGoogle ScholarPubMed
Lawrence, J. D. (1973). The ingestion of red blood cells by Schistosoma mansoni. Journal of Parasitology 59, 60–3.CrossRefGoogle ScholarPubMed
Lechene, C. P. & Warner, R. R. (1979). Electron probe analysis of liquid microdroplets. In Microbeam Analysis in Biology, (ed. Lechene, C. P. and Warner, R. R.), pp. 279–296. New York: Academic Press.Google Scholar
Lennox, R. W. & Schiller, E. L. (1972). Changes in dry weight and glycogen content as criteria for measuring the postcercarial growth and development of Schistosoma mansoni. Journal of Parasitology 58, 489–94.CrossRefGoogle ScholarPubMed
Lincicome, D. R., Eni, E. U. & Stirewalt, M. A. (1963). Dry weight and nitrogen content of Schistosoma mansoni from experimental infections. AIBS Bulletin 13, 75–6.CrossRefGoogle Scholar
Ma, L. (1963). Trace elements and polyphenol oxidase in Clonorchis sinensis. Journal of Parasitology 49, 197–203.CrossRefGoogle ScholarPubMed
Morgan, A. J., Davies, T. W. & Erasmus, D. A. (1975). Analysis of droplets from iso-atomic solutions as a means of calibrating a transmission electron analytical microscope (TEAM). Journal of Microscopy 104, 271–80.CrossRefGoogle Scholar
Morgan, A. J. & Davies, T. W. (1982). An electron microprobe study of the influence of beam current density on the stability of detectable elements in mixed salts (isoatomic) microdoplets. Journal of Microscopy 125, 103–16.CrossRefGoogle Scholar
Pax, R. A., Bennett, J. L. & Fetterer, R. H. (1978). A benzodiazepine derivative and praziquantel: effects on musculature of Schistosoma mansoni and Schistosoma japonicum. Naunyn-Schmiedeberg's Archiv für Pharmakologie 34, 309–15.CrossRefGoogle Scholar
Popiel, I. & Erasmus, D. A. (1981). Schistosoma mansoni: changes in the rate of tyrosine uptake by unisexual females after stimulation by males and male extracts. Journal of Helminthology 55, 33–7.CrossRefGoogle ScholarPubMed
Radke, M. G., Schneideh, M. D. & Houghtaling, D. G. (1957). Dry weight, nitrogen and phosphorus content of Schistosoma mansoni. Experimental Parasitology 6, 202–7.CrossRefGoogle ScholarPubMed
Shaw, J. R. (1977). Schistosoma mansoni: pairing in vitro and development of females from single sex infections. Experimental Parasitology 41, 54–65.CrossRefGoogle ScholarPubMed
Shaw, J. R. & Erasmus, D. A. (1981). Schistosoma mansoni: an examination of the reproductive status from single sex infections. Parasitology 82, 121–4.CrossRefGoogle ScholarPubMed
Shaw, J. R., Marshall, I. & Erasmus, D. A. (1977). Schistosoma mansoni: in vitro stimulation of vitelline cell development by extracts of male worms. Experimental Parasitology 42, 14–20.CrossRefGoogle ScholarPubMed
Shaw, M. K., Davies, T. W. & Erasmus, D. A. (1982). The demonstration of calcium in the axonal granules of the peripheral nervous system of male Schistosoma mansoni by X-ray microanalysis. Cell and Tissue Research 222, 597–604.CrossRefGoogle ScholarPubMed
Shaw, M. K. & Erasmus, D. A. (1982). Schistosoma mansoni: the presence and ultrastructure of vitelline cells in adult males. Journal of Helminthology 56, 51–3.CrossRefGoogle ScholarPubMed
Simkiss, K. (1976). Intracellular routes in biomineralization. In Calcium in Biological Systems, Symposia for the Society for Experimental Biology vol. 30 (ed. Duncan, C. J.), pp. 423–444. London and Cambridge: Cambridge University Press.Google Scholar
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Society for Experimental Biology No. 30 (1976). Calcium in Biological Systems (ed. Duncan, C. J.). Cambridge and London: Cambridge University Press.Google Scholar
Swiderski, Z. & Mokhtar, F. (1974). Étude de la vitellogénèse de Bothriocephalus clavibothrium Ariola, 1899 (Cestoda: Pseudophyllidea). Zeitschrift für Parasitenkunde 43, 135–49.CrossRefGoogle Scholar
Swiderski, Z. & Mackiewicz, J. S. (1976). Electron-microscope study of vitellogenesis in Glaridacris catostomi (Cestoda: Caryophyllidea). International Journal for Parasitology 6, 61–73.CrossRefGoogle ScholarPubMed
Trump, B. F., Berezesky, I. K. & Osornio-Vargas, A. R. (1981). Cell death and the disease process. The role of calcium. In Cell Death in Biology and Pathology (ed. Bowen, I. D. and Lockshin, R. A.), pp. 209–242. London and New York: Chapman and Hall.CrossRefGoogle Scholar
WoldeMussie, E. & Bennett, J. L. (1982). Plasma spectrometric analysis for Na, K, Ca, Mg, Fe and Cu in Schistosoma mansoni and S. japonicum. Journal of Parasitology 68, 48–52.CrossRefGoogle Scholar
WoldeMussie, E., Vande Waa, J., Pax, R. A., Fetterer, R. & Bennett, J. L. (1982). Schistosoma mansoni: calcium efflux and effects of calcium-free media on responses of the adult male musculature to praziquantel and other agents inducing contraction. Experimental Parasitology 53, 270–8.Google Scholar