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Schistosoma mansoni: analysis of the humoral and cellular basis of resistance in guinea-pigs vaccinated with radiation-attenuated cercariae

Published online by Cambridge University Press:  06 April 2009

D. J. McLaren ,
V. S. Delgado ,
J. R. Gordon  and
M. V. Rogers
D. J. McLaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
V. S. Delgado
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
J. R. Gordon
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
M. V. Rogers
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

This study addresses the humoral and cellular basis of specific acquired immunity in the guinea-pig irradiated vaccine model of schistosomiasis mansoni. Rodents vaccinated with 500 gamma-irradiated cercariae and then splenectomized 4·5 weeks later showed a 33% reduction in resistance to challenge as compared to vaccinated animals or vaccinated/sham splenectomized controls. Serum harvested from once vaccinated individuals conferred modest levels of resistance upon naive recipients in some experiments, but transfer was not achieved consistently. Serum from vaccinated and thrice boosted rodents (Vbbb) routinely transferred around 45% immunity, however, provided it was given in 4 ml aliquots on day 9 post-challenge; Vbbb serum thus transferred 50% of donor immunity. Interestingly, multiple doses of this protective serum given on and either side of day 9 did not enhance the protection achieved with a single 4 ml aliquot. Neither peripheral lymph node cells nor splenocytes from the polyvaccinated serum donors were able to transfer resistance to recipient guinea-pigs and they failed to augment the protection achieved with Vbbb serum. Food-pad testing revealed no correlation between delayed hypersensitivity responses and immunity to challenge in vaccinated guinea-pigs. Although polyvaccine guinea-pig serum successfully protected homologous recipients, it failed to protect mice when administered either at the time of challenge (the optimal schedule for transfer of polyvaccine mouse serum), or around day 9 (the optimal schedule for guinea-pigs). Similarly, guinea-pigs could not be protected with polyvaccine rat serum that conferred 75% resistance upon naive recipient rats.

Keywords

Schistosoma mansonivaccine immunitypassive transferguinea-pigssplenectomyhypersensitivity reactions
Type
Research Article
Information
Parasitology , Volume 100 , Issue 1 , February 1990 , pp. 35 - 44
Copyright
Copyright © Cambridge University Press 1990

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